Cellulolytic activity of anaerobic facultative fungi from the digestive tract of sheep fed with banana leaf hay
The addition of cellulolytic fungi, or their enzymes, in diets containing high levels of fiber are promising strategy for improving the performance. In this study, the aims were to select cellulolytic fungi from the digestive tract of sheep fed different concentrations of banana leaf hay. Thirty lam...
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| Format: | Article |
| Language: | English |
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Universidade Federal Rural do Semi-Árido (UFERSA)
2023-06-01
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| Series: | Acta Veterinaria Brasilica |
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| Online Access: | https://periodicos.ufersa.edu.br/acta/article/view/11015 |
| _version_ | 1797788675120037888 |
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| author | Cláudio Eduardo Silva Freitas Dorismar David Alves Angélica Alves de Moura Freitas Franklin Delano dos Santos Soares Enzo Freire Santana do Amaral Luciana Castro Gerassev Eduardo Robson Duarte |
| author_facet | Cláudio Eduardo Silva Freitas Dorismar David Alves Angélica Alves de Moura Freitas Franklin Delano dos Santos Soares Enzo Freire Santana do Amaral Luciana Castro Gerassev Eduardo Robson Duarte |
| author_sort | Cláudio Eduardo Silva Freitas |
| collection | DOAJ |
| description | The addition of cellulolytic fungi, or their enzymes, in diets containing high levels of fiber are promising strategy for improving the performance. In this study, the aims were to select cellulolytic fungi from the digestive tract of sheep fed different concentrations of banana leaf hay. Thirty lambs raised in a feedlot were evaluated, distributed in a completely randomized design, with diets containing 0, 125, 250, 375, or 500 g/Kg of dry matter and six replications. Approximately 15 mL of ruminal fluid and swabs from the rectal ampulla were collected. The cultures were carried out in a medium containing microcrystalline cellulose (C medium). The mycelial fungi isolates were identified through the microculture technique. Among the fungi from the ruminal fluid, 23 isolates corresponded to the genus Aspergillus and three to Paecilomyces spp. Among the isolates from the rectal ampulla, seven were A. spp., and three were P. spp. The A. genus predominated among the isolates from both evaluated sites (p <0.05). Fragments of these fungi were inoculated in triplicate in medium C at 37 °C and the cellulolytic activity index (CAI) was determined after 24, 48, and 72 hours of incubation. There was no difference in the CAI of Aspergillus spp. from animals fed different diets or of different evaluated sites (P > 0.05). However, 22 isolates of Aspergillus spp. and three of Paecilomyces spp. showed a CAI > 1, indicating biotechnological potential for cellulase production. These selected isolates could be selected for the elaboration of microbial additives in ruminant diets. |
| first_indexed | 2024-03-13T01:39:51Z |
| format | Article |
| id | doaj.art-78e842d84a8b4c81a118a9a4d98364c0 |
| institution | Directory Open Access Journal |
| issn | 1981-5484 |
| language | English |
| last_indexed | 2024-03-13T01:39:51Z |
| publishDate | 2023-06-01 |
| publisher | Universidade Federal Rural do Semi-Árido (UFERSA) |
| record_format | Article |
| series | Acta Veterinaria Brasilica |
| spelling | doaj.art-78e842d84a8b4c81a118a9a4d98364c02023-07-03T14:43:16ZengUniversidade Federal Rural do Semi-Árido (UFERSA)Acta Veterinaria Brasilica1981-54842023-06-01171283510.21708/avb.2023.17.1.11015Cellulolytic activity of anaerobic facultative fungi from the digestive tract of sheep fed with banana leaf hayCláudio Eduardo Silva Freitas0https://orcid.org/0000-0002-0037-7655Dorismar David Alves1https://orcid.org/0000-0003-4277-1498Angélica Alves de Moura Freitas2https://orcid.org/0000-0002-2040-8041Franklin Delano dos Santos Soares3https://orcid.org/0000-0001-7749-9338Enzo Freire Santana do Amaral4https://orcid.org/0000-0002-4093-6554Luciana Castro Gerassev5https://orcid.org/0000-0001-6906-4959Eduardo Robson Duarte6https://orcid.org/0000-0002-2205-9412Universidade Estadual de Montes Claros, UNIMONTES, Montes Claros, Minas Gerais, Brasil.Universidade Estadual de Montes Claros, UNIMONTES, Montes Claros, Minas Gerais, Brasil.Universidade Federal de Minas Gerais (UFMG), Instituto de Ciências Agrárias, Montes Claros, Minas Gerais, Brasil.Universidade Estadual de Montes Claros, UNIMONTES, Montes Claros, Minas Gerais, Brasil.Universidade Federal de Minas Gerais (UFMG), Escola de Medicina Veterinária, Belo Horizonte, Minas Gerais, Brasil.Universidade Federal de Minas Gerais (UFMG), Instituto de Ciências Agrárias, Montes Claros, Minas Gerais, Brasil.Universidade Federal de Minas Gerais (UFMG), Instituto de Ciências Agrárias, Montes Claros, Minas Gerais, Brasil.The addition of cellulolytic fungi, or their enzymes, in diets containing high levels of fiber are promising strategy for improving the performance. In this study, the aims were to select cellulolytic fungi from the digestive tract of sheep fed different concentrations of banana leaf hay. Thirty lambs raised in a feedlot were evaluated, distributed in a completely randomized design, with diets containing 0, 125, 250, 375, or 500 g/Kg of dry matter and six replications. Approximately 15 mL of ruminal fluid and swabs from the rectal ampulla were collected. The cultures were carried out in a medium containing microcrystalline cellulose (C medium). The mycelial fungi isolates were identified through the microculture technique. Among the fungi from the ruminal fluid, 23 isolates corresponded to the genus Aspergillus and three to Paecilomyces spp. Among the isolates from the rectal ampulla, seven were A. spp., and three were P. spp. The A. genus predominated among the isolates from both evaluated sites (p <0.05). Fragments of these fungi were inoculated in triplicate in medium C at 37 °C and the cellulolytic activity index (CAI) was determined after 24, 48, and 72 hours of incubation. There was no difference in the CAI of Aspergillus spp. from animals fed different diets or of different evaluated sites (P > 0.05). However, 22 isolates of Aspergillus spp. and three of Paecilomyces spp. showed a CAI > 1, indicating biotechnological potential for cellulase production. These selected isolates could be selected for the elaboration of microbial additives in ruminant diets.https://periodicos.ufersa.edu.br/acta/article/view/11015sheep productionalternative foodmusa paradisicacellulasesruminal microbiota |
| spellingShingle | Cláudio Eduardo Silva Freitas Dorismar David Alves Angélica Alves de Moura Freitas Franklin Delano dos Santos Soares Enzo Freire Santana do Amaral Luciana Castro Gerassev Eduardo Robson Duarte Cellulolytic activity of anaerobic facultative fungi from the digestive tract of sheep fed with banana leaf hay Acta Veterinaria Brasilica sheep production alternative food musa paradisica cellulases ruminal microbiota |
| title | Cellulolytic activity of anaerobic facultative fungi from the digestive tract of sheep fed with banana leaf hay |
| title_full | Cellulolytic activity of anaerobic facultative fungi from the digestive tract of sheep fed with banana leaf hay |
| title_fullStr | Cellulolytic activity of anaerobic facultative fungi from the digestive tract of sheep fed with banana leaf hay |
| title_full_unstemmed | Cellulolytic activity of anaerobic facultative fungi from the digestive tract of sheep fed with banana leaf hay |
| title_short | Cellulolytic activity of anaerobic facultative fungi from the digestive tract of sheep fed with banana leaf hay |
| title_sort | cellulolytic activity of anaerobic facultative fungi from the digestive tract of sheep fed with banana leaf hay |
| topic | sheep production alternative food musa paradisica cellulases ruminal microbiota |
| url | https://periodicos.ufersa.edu.br/acta/article/view/11015 |
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