Different Degrees of Sulfated Laminaria Polysaccharides Recovered Damaged HK-2 Cells and Inhibited Adhesion of Nano-COM and Nano-COD Crystals
Purpose. The crystal adhesion caused by the damage of renal tubular epithelial cells (HK-2) is the key to the formation of kidney stones. However, no effective preventive drug has been found. This study aims to explore the recovery effects of four Laminaria polysaccharides (SLPs) with different sulf...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Hindawi Limited
2024-01-01
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| Series: | Bioinorganic Chemistry and Applications |
| Online Access: | http://dx.doi.org/10.1155/2024/8843214 |
| _version_ | 1797360248455954432 |
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| author | Qiu-Shi Xu Zhi-Jian Wu Jian-Ming Sun Jing-Hong Liu Wei-Bo Huang Jian-Ming Ouyang |
| author_facet | Qiu-Shi Xu Zhi-Jian Wu Jian-Ming Sun Jing-Hong Liu Wei-Bo Huang Jian-Ming Ouyang |
| author_sort | Qiu-Shi Xu |
| collection | DOAJ |
| description | Purpose. The crystal adhesion caused by the damage of renal tubular epithelial cells (HK-2) is the key to the formation of kidney stones. However, no effective preventive drug has been found. This study aims to explore the recovery effects of four Laminaria polysaccharides (SLPs) with different sulfate (–OSO3–) contents on damaged HK-2 cells and the difference in the adhesion of damaged cells to nanometer calcium oxalate monohydrate (COM) and calcium oxalate dihydrate (COD) before and after recovery. Methods. Sodium oxalate (2.6 mmol/L) was used to damage HK-2 cells to establish a damaged model. SLPs (LP0, SLP1, SLP2, and SLP3) with –OSO3– contents of 0.73%, 15.1%, 22.8%, and 31.3%, respectively, were used to restore the damaged cells, and the effects of SLPs on the adhesion of COM and COD, with a size of about 100 nm before and after recovery, were measured. Results. The following results were observed after SLPs recovered the damaged HK-2 cells: increased cell viability, restored cell morphology, decreased reactive oxygen levels, increased mitochondrial membrane potential, decreased phosphatidylserine eversion ratio, increased cell migration ability, reduced expression of annexin A1, transmembrane protein, and heat shock protein 90 on the cell surface, and reduced adhesion amount of cells to COM and COD. Under the same conditions, the adhesion ability of cells to COD crystals was weaker than that to COM crystals. Conclusions. As the sulfate content in SLPs increases, the ability of SLPs to recover damaged HK-2 cells and inhibit crystal adhesion increases. SLP3 with high –OSO3– content may be a potential drug to prevent kidney stones. |
| first_indexed | 2024-03-08T15:35:41Z |
| format | Article |
| id | doaj.art-78ea07aee9dd45568dbd5f4b2000c60c |
| institution | Directory Open Access Journal |
| issn | 1687-479X |
| language | English |
| last_indexed | 2024-03-08T15:35:41Z |
| publishDate | 2024-01-01 |
| publisher | Hindawi Limited |
| record_format | Article |
| series | Bioinorganic Chemistry and Applications |
| spelling | doaj.art-78ea07aee9dd45568dbd5f4b2000c60c2024-01-10T00:00:06ZengHindawi LimitedBioinorganic Chemistry and Applications1687-479X2024-01-01202410.1155/2024/8843214Different Degrees of Sulfated Laminaria Polysaccharides Recovered Damaged HK-2 Cells and Inhibited Adhesion of Nano-COM and Nano-COD CrystalsQiu-Shi Xu0Zhi-Jian Wu1Jian-Ming Sun2Jing-Hong Liu3Wei-Bo Huang4Jian-Ming Ouyang5Department of UrologyDepartment of UrologyDepartment of UrologyInstitute of Biomineralization and Lithiasis ResearchInstitute of Biomineralization and Lithiasis ResearchInstitute of Biomineralization and Lithiasis ResearchPurpose. The crystal adhesion caused by the damage of renal tubular epithelial cells (HK-2) is the key to the formation of kidney stones. However, no effective preventive drug has been found. This study aims to explore the recovery effects of four Laminaria polysaccharides (SLPs) with different sulfate (–OSO3–) contents on damaged HK-2 cells and the difference in the adhesion of damaged cells to nanometer calcium oxalate monohydrate (COM) and calcium oxalate dihydrate (COD) before and after recovery. Methods. Sodium oxalate (2.6 mmol/L) was used to damage HK-2 cells to establish a damaged model. SLPs (LP0, SLP1, SLP2, and SLP3) with –OSO3– contents of 0.73%, 15.1%, 22.8%, and 31.3%, respectively, were used to restore the damaged cells, and the effects of SLPs on the adhesion of COM and COD, with a size of about 100 nm before and after recovery, were measured. Results. The following results were observed after SLPs recovered the damaged HK-2 cells: increased cell viability, restored cell morphology, decreased reactive oxygen levels, increased mitochondrial membrane potential, decreased phosphatidylserine eversion ratio, increased cell migration ability, reduced expression of annexin A1, transmembrane protein, and heat shock protein 90 on the cell surface, and reduced adhesion amount of cells to COM and COD. Under the same conditions, the adhesion ability of cells to COD crystals was weaker than that to COM crystals. Conclusions. As the sulfate content in SLPs increases, the ability of SLPs to recover damaged HK-2 cells and inhibit crystal adhesion increases. SLP3 with high –OSO3– content may be a potential drug to prevent kidney stones.http://dx.doi.org/10.1155/2024/8843214 |
| spellingShingle | Qiu-Shi Xu Zhi-Jian Wu Jian-Ming Sun Jing-Hong Liu Wei-Bo Huang Jian-Ming Ouyang Different Degrees of Sulfated Laminaria Polysaccharides Recovered Damaged HK-2 Cells and Inhibited Adhesion of Nano-COM and Nano-COD Crystals Bioinorganic Chemistry and Applications |
| title | Different Degrees of Sulfated Laminaria Polysaccharides Recovered Damaged HK-2 Cells and Inhibited Adhesion of Nano-COM and Nano-COD Crystals |
| title_full | Different Degrees of Sulfated Laminaria Polysaccharides Recovered Damaged HK-2 Cells and Inhibited Adhesion of Nano-COM and Nano-COD Crystals |
| title_fullStr | Different Degrees of Sulfated Laminaria Polysaccharides Recovered Damaged HK-2 Cells and Inhibited Adhesion of Nano-COM and Nano-COD Crystals |
| title_full_unstemmed | Different Degrees of Sulfated Laminaria Polysaccharides Recovered Damaged HK-2 Cells and Inhibited Adhesion of Nano-COM and Nano-COD Crystals |
| title_short | Different Degrees of Sulfated Laminaria Polysaccharides Recovered Damaged HK-2 Cells and Inhibited Adhesion of Nano-COM and Nano-COD Crystals |
| title_sort | different degrees of sulfated laminaria polysaccharides recovered damaged hk 2 cells and inhibited adhesion of nano com and nano cod crystals |
| url | http://dx.doi.org/10.1155/2024/8843214 |
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