Heterogeneity evaluation of multi-high b-value apparent diffusion coefficient on cerebral ischemia in MCAO rat
PurposeTo assess brain damage in a rat model of cerebral ischemia based on apparent diffusion coefficient (ADC) data obtained from multi-high b-values and evaluate the relationship between Aquaporin 4 (AQP4) expression and ADC.MethodsThirty eight male Sprague–Dawley rats were randomized into two gro...
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnins.2022.1048429/full |
| _version_ | 1797979947639242752 |
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| author | Liwei Shi Liwei Shi Liwei Shi Bo Yu Bo Yu Qiuyan Chen Qiuyan Chen Tianxiu Zheng Tianxiu Zheng Peiqiu Xing Peiqiu Xing Dingtai Wei Dingtai Wei Dingtai Wei |
| author_facet | Liwei Shi Liwei Shi Liwei Shi Bo Yu Bo Yu Qiuyan Chen Qiuyan Chen Tianxiu Zheng Tianxiu Zheng Peiqiu Xing Peiqiu Xing Dingtai Wei Dingtai Wei Dingtai Wei |
| author_sort | Liwei Shi |
| collection | DOAJ |
| description | PurposeTo assess brain damage in a rat model of cerebral ischemia based on apparent diffusion coefficient (ADC) data obtained from multi-high b-values and evaluate the relationship between Aquaporin 4 (AQP4) expression and ADC.MethodsThirty eight male Sprague–Dawley rats were randomized into two groups: (1) sham controls (n = 6) and (2) cerebral ischemia (successful model, n = 19). All rats underwent diffusion-weighted imaging (DWI) with both standard b-values and multi-high b-values (2,500–4,500 s/mm2) using a 3.0-T device. Standard ADC (ADCst) maps and multi-high b-value ADCs (ADCmh) were calculated, respectively. Aquaporin 4 expression was quantified using Western blot. Relative values of ADCst and ADCmh, AQP4 expression were compared between the sham group and the ischemia group. Correlations between ADC values and AQP4 expression were evaluated.ResultsAt 0.5 h after suture insertion, the value of ADCmh on the lesion was obviously decreased, and there was no difference in lesion volume when compared with ADCst. After reperfusion, besides similar regions where ADCst values decreased, we also found additional large values on ADCmh within the cortex of the ipsilateral side or surrounding the lesion. The lesion evolution of the large value on ADCmh was quite different from other indicators. But the total ADCmh values were still significantly associated with ADCst. The AQP4 protein expression level was appreciably increased after middle cerebral artery occlusion (MCAO), but there was no correlation between AQP4 expression either with ADCmh or ADCst.ConclusionWe found the large values on ADCmh during the progression of cerebral infarction is varied, but there was no correlation between ADCmh values and AQP4 expression. ADCmh may indicate the heterogeneity of ischemia lesions, but the underlying pathological basis should be further explored. |
| first_indexed | 2024-04-11T05:46:46Z |
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| id | doaj.art-78ec0d5a78d74390a7759cc4b2ff2b66 |
| institution | Directory Open Access Journal |
| issn | 1662-453X |
| language | English |
| last_indexed | 2024-04-11T05:46:46Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Neuroscience |
| spelling | doaj.art-78ec0d5a78d74390a7759cc4b2ff2b662022-12-22T04:42:12ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2022-12-011610.3389/fnins.2022.10484291048429Heterogeneity evaluation of multi-high b-value apparent diffusion coefficient on cerebral ischemia in MCAO ratLiwei Shi0Liwei Shi1Liwei Shi2Bo Yu3Bo Yu4Qiuyan Chen5Qiuyan Chen6Tianxiu Zheng7Tianxiu Zheng8Peiqiu Xing9Peiqiu Xing10Dingtai Wei11Dingtai Wei12Dingtai Wei13The Third Clinical Medical College, Fujian Medical University, Fuzhou, Fujian, ChinaDepartment of Radiology, Ningde Municipal Hospital of Ningde Normal University, Ningde, Fujian, ChinaFunctional and Molecular Imaging Laboratory for Cerebral Vascular Diseases, Ningde Municipal Hospital of Ningde Normal University, Ningde, Fujian, ChinaDepartment of Radiology, Ningde Municipal Hospital of Ningde Normal University, Ningde, Fujian, ChinaFunctional and Molecular Imaging Laboratory for Cerebral Vascular Diseases, Ningde Municipal Hospital of Ningde Normal University, Ningde, Fujian, ChinaDepartment of Radiology, Ningde Municipal Hospital of Ningde Normal University, Ningde, Fujian, ChinaFunctional and Molecular Imaging Laboratory for Cerebral Vascular Diseases, Ningde Municipal Hospital of Ningde Normal University, Ningde, Fujian, ChinaDepartment of Radiology, Ningde Municipal Hospital of Ningde Normal University, Ningde, Fujian, ChinaFunctional and Molecular Imaging Laboratory for Cerebral Vascular Diseases, Ningde Municipal Hospital of Ningde Normal University, Ningde, Fujian, ChinaDepartment of Radiology, Ningde Municipal Hospital of Ningde Normal University, Ningde, Fujian, ChinaFunctional and Molecular Imaging Laboratory for Cerebral Vascular Diseases, Ningde Municipal Hospital of Ningde Normal University, Ningde, Fujian, ChinaThe Third Clinical Medical College, Fujian Medical University, Fuzhou, Fujian, ChinaDepartment of Radiology, Ningde Municipal Hospital of Ningde Normal University, Ningde, Fujian, ChinaFunctional and Molecular Imaging Laboratory for Cerebral Vascular Diseases, Ningde Municipal Hospital of Ningde Normal University, Ningde, Fujian, ChinaPurposeTo assess brain damage in a rat model of cerebral ischemia based on apparent diffusion coefficient (ADC) data obtained from multi-high b-values and evaluate the relationship between Aquaporin 4 (AQP4) expression and ADC.MethodsThirty eight male Sprague–Dawley rats were randomized into two groups: (1) sham controls (n = 6) and (2) cerebral ischemia (successful model, n = 19). All rats underwent diffusion-weighted imaging (DWI) with both standard b-values and multi-high b-values (2,500–4,500 s/mm2) using a 3.0-T device. Standard ADC (ADCst) maps and multi-high b-value ADCs (ADCmh) were calculated, respectively. Aquaporin 4 expression was quantified using Western blot. Relative values of ADCst and ADCmh, AQP4 expression were compared between the sham group and the ischemia group. Correlations between ADC values and AQP4 expression were evaluated.ResultsAt 0.5 h after suture insertion, the value of ADCmh on the lesion was obviously decreased, and there was no difference in lesion volume when compared with ADCst. After reperfusion, besides similar regions where ADCst values decreased, we also found additional large values on ADCmh within the cortex of the ipsilateral side or surrounding the lesion. The lesion evolution of the large value on ADCmh was quite different from other indicators. But the total ADCmh values were still significantly associated with ADCst. The AQP4 protein expression level was appreciably increased after middle cerebral artery occlusion (MCAO), but there was no correlation between AQP4 expression either with ADCmh or ADCst.ConclusionWe found the large values on ADCmh during the progression of cerebral infarction is varied, but there was no correlation between ADCmh values and AQP4 expression. ADCmh may indicate the heterogeneity of ischemia lesions, but the underlying pathological basis should be further explored.https://www.frontiersin.org/articles/10.3389/fnins.2022.1048429/fulldiffusionischemiaAquaporin 4MRIrat |
| spellingShingle | Liwei Shi Liwei Shi Liwei Shi Bo Yu Bo Yu Qiuyan Chen Qiuyan Chen Tianxiu Zheng Tianxiu Zheng Peiqiu Xing Peiqiu Xing Dingtai Wei Dingtai Wei Dingtai Wei Heterogeneity evaluation of multi-high b-value apparent diffusion coefficient on cerebral ischemia in MCAO rat Frontiers in Neuroscience diffusion ischemia Aquaporin 4 MRI rat |
| title | Heterogeneity evaluation of multi-high b-value apparent diffusion coefficient on cerebral ischemia in MCAO rat |
| title_full | Heterogeneity evaluation of multi-high b-value apparent diffusion coefficient on cerebral ischemia in MCAO rat |
| title_fullStr | Heterogeneity evaluation of multi-high b-value apparent diffusion coefficient on cerebral ischemia in MCAO rat |
| title_full_unstemmed | Heterogeneity evaluation of multi-high b-value apparent diffusion coefficient on cerebral ischemia in MCAO rat |
| title_short | Heterogeneity evaluation of multi-high b-value apparent diffusion coefficient on cerebral ischemia in MCAO rat |
| title_sort | heterogeneity evaluation of multi high b value apparent diffusion coefficient on cerebral ischemia in mcao rat |
| topic | diffusion ischemia Aquaporin 4 MRI rat |
| url | https://www.frontiersin.org/articles/10.3389/fnins.2022.1048429/full |
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