Study of Genetic Diversity among Simmental Cross Cattle in West Sumatra Based on Microsatellite Markers
A study was conducted to assess the genetic diversity among Simmental Cross cattle in West Sumatra using microsatellite DNA markers. A total of 176 individual cattle blood samples was used for obtaining DNA samples. Twelve primers of microsatellite loci as recommended by FAO were used to identify th...
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2016-02-01
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| Series: | Asian-Australasian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | http://www.ajas.info/upload/pdf/ajas-29-2-196.pdf |
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| author | Paskah Partogi Agung Ferdy Saputra Wike Andre Septian Lusiana Moch. Syamsul Arifin Zein Sri Sulandari Saiful Anwar Ari Sulistyo Wulandari Syahruddin Said Baharuddin Tappa |
| author_facet | Paskah Partogi Agung Ferdy Saputra Wike Andre Septian Lusiana Moch. Syamsul Arifin Zein Sri Sulandari Saiful Anwar Ari Sulistyo Wulandari Syahruddin Said Baharuddin Tappa |
| author_sort | Paskah Partogi Agung |
| collection | DOAJ |
| description | A study was conducted to assess the genetic diversity among Simmental Cross cattle in West Sumatra using microsatellite DNA markers. A total of 176 individual cattle blood samples was used for obtaining DNA samples. Twelve primers of microsatellite loci as recommended by FAO were used to identify the genetic diversity of the Simmental Cross cattle population. Multiplex DNA fragment analysis method was used for allele identification. All the microsatellite loci in this study were highly polymorphic and all of the identified alleles were able to classify the cattle population into several groups based on their genetic distance. The heterozygosity values of microsatellite loci in this study ranged from 0.556 to 0.782. The polymorphism information content (PIC) value of the 12 observed loci is high (PIC>0.5). The highest PIC value in the Simmental cattle population was 0.893 (locus TGLA53), while the lowest value was 0.529 (locus BM1818). Based on the genetic distance value, the subpopulation of the Simmental Cross-Agam and the Simmental Cross-Limapuluh Kota was exceptionally close to the Simmental Purebred thus indicating that a grading-up process has taken place with the Simmental Purebred. In view of the advantages possessed by the Simmental Cross cattle and the evaluation of the genetic diversity results, a number of subpopulations in this study can be considered as the initial (base) population for the Simmental Cross cattle breeding programs in West Sumatra, Indonesia. |
| first_indexed | 2024-12-14T02:45:23Z |
| format | Article |
| id | doaj.art-78eccfc254304ceeabd362c57169bfbf |
| institution | Directory Open Access Journal |
| issn | 1011-2367 1976-5517 |
| language | English |
| last_indexed | 2024-12-14T02:45:23Z |
| publishDate | 2016-02-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Asian-Australasian Journal of Animal Sciences |
| spelling | doaj.art-78eccfc254304ceeabd362c57169bfbf2022-12-21T23:19:54ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172016-02-0129217618310.5713/ajas.15.015523324Study of Genetic Diversity among Simmental Cross Cattle in West Sumatra Based on Microsatellite MarkersPaskah Partogi AgungFerdy SaputraWike Andre SeptianLusianaMoch. Syamsul Arifin Zein0Sri Sulandari1Saiful AnwarAri Sulistyo WulandariSyahruddin SaidBaharuddin Tappa Research Center for Biology-Indonesian Institute of Sciences, Cibinong 16911, West Java, .Indonesia Research Center for Biology-Indonesian Institute of Sciences, Cibinong 16911, West Java, .IndonesiaA study was conducted to assess the genetic diversity among Simmental Cross cattle in West Sumatra using microsatellite DNA markers. A total of 176 individual cattle blood samples was used for obtaining DNA samples. Twelve primers of microsatellite loci as recommended by FAO were used to identify the genetic diversity of the Simmental Cross cattle population. Multiplex DNA fragment analysis method was used for allele identification. All the microsatellite loci in this study were highly polymorphic and all of the identified alleles were able to classify the cattle population into several groups based on their genetic distance. The heterozygosity values of microsatellite loci in this study ranged from 0.556 to 0.782. The polymorphism information content (PIC) value of the 12 observed loci is high (PIC>0.5). The highest PIC value in the Simmental cattle population was 0.893 (locus TGLA53), while the lowest value was 0.529 (locus BM1818). Based on the genetic distance value, the subpopulation of the Simmental Cross-Agam and the Simmental Cross-Limapuluh Kota was exceptionally close to the Simmental Purebred thus indicating that a grading-up process has taken place with the Simmental Purebred. In view of the advantages possessed by the Simmental Cross cattle and the evaluation of the genetic diversity results, a number of subpopulations in this study can be considered as the initial (base) population for the Simmental Cross cattle breeding programs in West Sumatra, Indonesia.http://www.ajas.info/upload/pdf/ajas-29-2-196.pdfSimmentalWest SumatraMicrosatelliteGenetic Diversity |
| spellingShingle | Paskah Partogi Agung Ferdy Saputra Wike Andre Septian Lusiana Moch. Syamsul Arifin Zein Sri Sulandari Saiful Anwar Ari Sulistyo Wulandari Syahruddin Said Baharuddin Tappa Study of Genetic Diversity among Simmental Cross Cattle in West Sumatra Based on Microsatellite Markers Asian-Australasian Journal of Animal Sciences Simmental West Sumatra Microsatellite Genetic Diversity |
| title | Study of Genetic Diversity among Simmental Cross Cattle in West Sumatra Based on Microsatellite Markers |
| title_full | Study of Genetic Diversity among Simmental Cross Cattle in West Sumatra Based on Microsatellite Markers |
| title_fullStr | Study of Genetic Diversity among Simmental Cross Cattle in West Sumatra Based on Microsatellite Markers |
| title_full_unstemmed | Study of Genetic Diversity among Simmental Cross Cattle in West Sumatra Based on Microsatellite Markers |
| title_short | Study of Genetic Diversity among Simmental Cross Cattle in West Sumatra Based on Microsatellite Markers |
| title_sort | study of genetic diversity among simmental cross cattle in west sumatra based on microsatellite markers |
| topic | Simmental West Sumatra Microsatellite Genetic Diversity |
| url | http://www.ajas.info/upload/pdf/ajas-29-2-196.pdf |
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