Molecular Characterization, Tissue Distribution Profile, and Nutritional Regulation of <i>acsl</i> Gene Family in Golden Pompano (<i>Trachinotus ovatus</i>)
Long chain acyl-coA synthase (<i>acsl</i>) family genes activate the conversion of long chain fatty acids into acyl-coA to regulate fatty acid metabolism. However, the evolutionary characteristics, tissue expression and nutritional regulation of the <i>acsl</i> gene family ar...
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MDPI AG
2022-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/12/6437 |
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| author | Zhigang Yang Hangbo Zhu Xiaoping Huang Aimin Wang Dizhi Xie |
| author_facet | Zhigang Yang Hangbo Zhu Xiaoping Huang Aimin Wang Dizhi Xie |
| author_sort | Zhigang Yang |
| collection | DOAJ |
| description | Long chain acyl-coA synthase (<i>acsl</i>) family genes activate the conversion of long chain fatty acids into acyl-coA to regulate fatty acid metabolism. However, the evolutionary characteristics, tissue expression and nutritional regulation of the <i>acsl</i> gene family are poorly understood in fish. The present study investigated the molecular characterization, tissue expression and nutritional regulation of the <i>acsl</i> gene family in golden pompano (<i>Trachinotus ovatus</i>). The results showed that the coding regions of <i>acsl1</i>, <i>acsl3</i>, <i>acsl4</i>, <i>acsl5</i> and <i>acsl6</i> cDNA were 2091 bp, 2142 bp, 2136 bp, 1977 bp and 2007 bp, encoding 697, 714, 712, 659 and 669 amino acids, respectively. Five <i>acsl</i> isoforms divided into two branches, namely, <i>acsl1</i>, <i>acsl5</i> and <i>acsl6</i>, as well as <i>acsl3</i> and <i>acsl4</i>. The tissue expression distribution of <i>acsl</i> genes showed that <i>acsl1</i> and <i>acsl3</i> are widely expressed in the detected tissues, while <i>acsl4</i>, <i>acsl5</i> and <i>acsl6</i> are mainly expressed in the brain. Compared to the fish fed with lard oil diets, the fish fed with soybean oil exhibited high muscular C<sub>18</sub> PUFA contents and <i>acsl1</i> and <i>acsl3</i> mRNA levels, as well as low muscular SFA contents and <i>acsl4</i> mRNA levels. High muscular n-3 LC-PUFA contents, and <i>acsl3</i>, <i>acsl4</i> and <i>acsl6</i> mRNA levels were observed in the fish fed with fish oil diets compared with those of fish fed with lard oil or soybean oil diets. High n-3 LC-PUFA levels and DHA contents, as well as the <i>acsl3</i>, <i>acsl4</i> and <i>acsl6</i> mRNA levels were exhibited in the muscle of fish fed diets with high dietary n-3 LC-PUFA levels. Additionally, the muscular <i>acsl3</i>, <i>acsl4</i> and <i>acsl6</i> mRNA expression levels, n-3 LC-PUFA and DHA levels were significantly up-regulated by the increase of dietary DHA proportions. Collectively, the positive relationship among dietary fatty acids, muscular fatty acids and <i>acsl</i> mRNA, indicated that <i>T. ovatus</i> Acsl1 and Acsl3 are beneficial for the C<sub>18</sub> PUFA enrichment, and Acsl3, Acsl4 and Acsl6 are for n-3 LC-PUFA and DHA enrichment. The acquisition of fish Acsl potential function in the present study will play the foundation for ameliorating the fatty acids nutrition in farmed fish products. |
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| language | English |
| last_indexed | 2024-03-09T23:36:07Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-78ed30c68b6c4302998d986c1a428bd42023-11-23T17:00:41ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-06-012312643710.3390/ijms23126437Molecular Characterization, Tissue Distribution Profile, and Nutritional Regulation of <i>acsl</i> Gene Family in Golden Pompano (<i>Trachinotus ovatus</i>)Zhigang Yang0Hangbo Zhu1Xiaoping Huang2Aimin Wang3Dizhi Xie4Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, ChinaKey Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, ChinaCollege of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng 224000, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, ChinaLong chain acyl-coA synthase (<i>acsl</i>) family genes activate the conversion of long chain fatty acids into acyl-coA to regulate fatty acid metabolism. However, the evolutionary characteristics, tissue expression and nutritional regulation of the <i>acsl</i> gene family are poorly understood in fish. The present study investigated the molecular characterization, tissue expression and nutritional regulation of the <i>acsl</i> gene family in golden pompano (<i>Trachinotus ovatus</i>). The results showed that the coding regions of <i>acsl1</i>, <i>acsl3</i>, <i>acsl4</i>, <i>acsl5</i> and <i>acsl6</i> cDNA were 2091 bp, 2142 bp, 2136 bp, 1977 bp and 2007 bp, encoding 697, 714, 712, 659 and 669 amino acids, respectively. Five <i>acsl</i> isoforms divided into two branches, namely, <i>acsl1</i>, <i>acsl5</i> and <i>acsl6</i>, as well as <i>acsl3</i> and <i>acsl4</i>. The tissue expression distribution of <i>acsl</i> genes showed that <i>acsl1</i> and <i>acsl3</i> are widely expressed in the detected tissues, while <i>acsl4</i>, <i>acsl5</i> and <i>acsl6</i> are mainly expressed in the brain. Compared to the fish fed with lard oil diets, the fish fed with soybean oil exhibited high muscular C<sub>18</sub> PUFA contents and <i>acsl1</i> and <i>acsl3</i> mRNA levels, as well as low muscular SFA contents and <i>acsl4</i> mRNA levels. High muscular n-3 LC-PUFA contents, and <i>acsl3</i>, <i>acsl4</i> and <i>acsl6</i> mRNA levels were observed in the fish fed with fish oil diets compared with those of fish fed with lard oil or soybean oil diets. High n-3 LC-PUFA levels and DHA contents, as well as the <i>acsl3</i>, <i>acsl4</i> and <i>acsl6</i> mRNA levels were exhibited in the muscle of fish fed diets with high dietary n-3 LC-PUFA levels. Additionally, the muscular <i>acsl3</i>, <i>acsl4</i> and <i>acsl6</i> mRNA expression levels, n-3 LC-PUFA and DHA levels were significantly up-regulated by the increase of dietary DHA proportions. Collectively, the positive relationship among dietary fatty acids, muscular fatty acids and <i>acsl</i> mRNA, indicated that <i>T. ovatus</i> Acsl1 and Acsl3 are beneficial for the C<sub>18</sub> PUFA enrichment, and Acsl3, Acsl4 and Acsl6 are for n-3 LC-PUFA and DHA enrichment. The acquisition of fish Acsl potential function in the present study will play the foundation for ameliorating the fatty acids nutrition in farmed fish products.https://www.mdpi.com/1422-0067/23/12/6437<i>acsl</i> gene familygolden pompanotissue expressionnutritional regulationLC-PUFA |
| spellingShingle | Zhigang Yang Hangbo Zhu Xiaoping Huang Aimin Wang Dizhi Xie Molecular Characterization, Tissue Distribution Profile, and Nutritional Regulation of <i>acsl</i> Gene Family in Golden Pompano (<i>Trachinotus ovatus</i>) International Journal of Molecular Sciences <i>acsl</i> gene family golden pompano tissue expression nutritional regulation LC-PUFA |
| title | Molecular Characterization, Tissue Distribution Profile, and Nutritional Regulation of <i>acsl</i> Gene Family in Golden Pompano (<i>Trachinotus ovatus</i>) |
| title_full | Molecular Characterization, Tissue Distribution Profile, and Nutritional Regulation of <i>acsl</i> Gene Family in Golden Pompano (<i>Trachinotus ovatus</i>) |
| title_fullStr | Molecular Characterization, Tissue Distribution Profile, and Nutritional Regulation of <i>acsl</i> Gene Family in Golden Pompano (<i>Trachinotus ovatus</i>) |
| title_full_unstemmed | Molecular Characterization, Tissue Distribution Profile, and Nutritional Regulation of <i>acsl</i> Gene Family in Golden Pompano (<i>Trachinotus ovatus</i>) |
| title_short | Molecular Characterization, Tissue Distribution Profile, and Nutritional Regulation of <i>acsl</i> Gene Family in Golden Pompano (<i>Trachinotus ovatus</i>) |
| title_sort | molecular characterization tissue distribution profile and nutritional regulation of i acsl i gene family in golden pompano i trachinotus ovatus i |
| topic | <i>acsl</i> gene family golden pompano tissue expression nutritional regulation LC-PUFA |
| url | https://www.mdpi.com/1422-0067/23/12/6437 |
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