Age, breed, sex and diet influence serum metabolite profiles of 2000 pet dogs
As an individual's metabolism reflects health and disease states well, metabolomics holds a vast potential in biomedical applications. However, normal physiological factors, such as age, can also influence metabolism, challenging the establishment of disease-specific metabolic aberrations. Here...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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The Royal Society
2022-02-01
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| Series: | Royal Society Open Science |
| Subjects: | |
| Online Access: | https://royalsocietypublishing.org/doi/10.1098/rsos.211642 |
| _version_ | 1797837642438541312 |
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| author | Jenni Puurunen Claudia Ottka Milla Salonen Julia E. Niskanen Hannes Lohi |
| author_facet | Jenni Puurunen Claudia Ottka Milla Salonen Julia E. Niskanen Hannes Lohi |
| author_sort | Jenni Puurunen |
| collection | DOAJ |
| description | As an individual's metabolism reflects health and disease states well, metabolomics holds a vast potential in biomedical applications. However, normal physiological factors, such as age, can also influence metabolism, challenging the establishment of disease-specific metabolic aberrations. Here, we examined how physiological and diet-related factors drive variance in the metabolism of healthy pet dogs. We analysed 2068 serum samples using a canine nuclear magnetic resonance (NMR) spectroscopy-based metabolomics platform. With generalized linear models, we discovered that age, breed, sex, sterilization, diet type and fasting time significantly affected the canine metabolite profiles. Especially, breed and age caused considerable variation in the metabolite concentrations, and breeds with very different body conformations systematically differed in several lipid measurands. Our results enhance the understanding how normal physiological factors influence canine metabolism, aid accurate interpretation of the NMR results, and suggest the NMR platform might be applied in identifying aberrations in nutrient absorption and metabolism. |
| first_indexed | 2024-04-09T15:27:59Z |
| format | Article |
| id | doaj.art-78f071aa9af24a8184b98aec75368782 |
| institution | Directory Open Access Journal |
| issn | 2054-5703 |
| language | English |
| last_indexed | 2024-04-09T15:27:59Z |
| publishDate | 2022-02-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Royal Society Open Science |
| spelling | doaj.art-78f071aa9af24a8184b98aec753687822023-04-28T11:04:40ZengThe Royal SocietyRoyal Society Open Science2054-57032022-02-019210.1098/rsos.211642Age, breed, sex and diet influence serum metabolite profiles of 2000 pet dogsJenni Puurunen0Claudia Ottka1Milla Salonen2Julia E. Niskanen3Hannes Lohi4PetBiomics Ltd, 00300 Helsinki, FinlandPetBiomics Ltd, 00300 Helsinki, FinlandDepartment of Veterinary Biosciences, University of Helsinki, 00014 Helsinki, FinlandDepartment of Veterinary Biosciences, University of Helsinki, 00014 Helsinki, FinlandPetBiomics Ltd, 00300 Helsinki, FinlandAs an individual's metabolism reflects health and disease states well, metabolomics holds a vast potential in biomedical applications. However, normal physiological factors, such as age, can also influence metabolism, challenging the establishment of disease-specific metabolic aberrations. Here, we examined how physiological and diet-related factors drive variance in the metabolism of healthy pet dogs. We analysed 2068 serum samples using a canine nuclear magnetic resonance (NMR) spectroscopy-based metabolomics platform. With generalized linear models, we discovered that age, breed, sex, sterilization, diet type and fasting time significantly affected the canine metabolite profiles. Especially, breed and age caused considerable variation in the metabolite concentrations, and breeds with very different body conformations systematically differed in several lipid measurands. Our results enhance the understanding how normal physiological factors influence canine metabolism, aid accurate interpretation of the NMR results, and suggest the NMR platform might be applied in identifying aberrations in nutrient absorption and metabolism.https://royalsocietypublishing.org/doi/10.1098/rsos.211642canine metabolismcanine physiologynuclear magnetic resonance metabolomicsmetabolites |
| spellingShingle | Jenni Puurunen Claudia Ottka Milla Salonen Julia E. Niskanen Hannes Lohi Age, breed, sex and diet influence serum metabolite profiles of 2000 pet dogs Royal Society Open Science canine metabolism canine physiology nuclear magnetic resonance metabolomics metabolites |
| title | Age, breed, sex and diet influence serum metabolite profiles of 2000 pet dogs |
| title_full | Age, breed, sex and diet influence serum metabolite profiles of 2000 pet dogs |
| title_fullStr | Age, breed, sex and diet influence serum metabolite profiles of 2000 pet dogs |
| title_full_unstemmed | Age, breed, sex and diet influence serum metabolite profiles of 2000 pet dogs |
| title_short | Age, breed, sex and diet influence serum metabolite profiles of 2000 pet dogs |
| title_sort | age breed sex and diet influence serum metabolite profiles of 2000 pet dogs |
| topic | canine metabolism canine physiology nuclear magnetic resonance metabolomics metabolites |
| url | https://royalsocietypublishing.org/doi/10.1098/rsos.211642 |
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