Design of a Chimeric Multi-Epitope Vaccine (CMEV) against Both <i>Leishmania martiniquensis</i> and <i>Leishmania orientalis</i> Parasites Using Immunoinformatic Approaches
Leishmaniasis is a parasitic disease caused by protozoan flagellates of the genus <i>Leishmania</i>. Recently, <i>Leishmania martiniquensis</i> and <i>Leishmania orientalis</i>, emerging species of <i>Leishmania</i>, were isolated from patients in Thai...
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MDPI AG
2022-10-01
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| author | Kentaro Imaizumi Thararat Phurahong Suradej Siripattanapipong Kiattawee Choowongkomon Saovanee Leelayoova Mathirut Mungthin Teerasak E-kobon Sasimanas Unajak |
| author_facet | Kentaro Imaizumi Thararat Phurahong Suradej Siripattanapipong Kiattawee Choowongkomon Saovanee Leelayoova Mathirut Mungthin Teerasak E-kobon Sasimanas Unajak |
| author_sort | Kentaro Imaizumi |
| collection | DOAJ |
| description | Leishmaniasis is a parasitic disease caused by protozoan flagellates of the genus <i>Leishmania</i>. Recently, <i>Leishmania martiniquensis</i> and <i>Leishmania orientalis</i>, emerging species of <i>Leishmania</i>, were isolated from patients in Thailand. Development of the vaccine is demanded; however, genetic differences between the two species make it difficult to design a vaccine that is effective for both species. In this study, we applied immuno-informatic approaches to design a chimeric multi-epitope vaccine (CMEV) against both <i>L. martiniquensis</i> and <i>L. orientalis</i>. We identified seven helper T lymphocyte (HTL) epitopes, sixteen cytotoxic T lymphocyte (CTL) epitopes, and eleven B-cell epitopes from sixteen conserved antigenic proteins found in both species. All these epitopes were joined together, and to further enhance immunogenicity, protein and peptides adjuvant were also added at the N-terminal of the molecule by using specific linkers. The candidate CMEV was subsequently analyzed from the perspectives of the antigenicity, allergenicity, and physiochemical properties. The interaction of the designed multi-epitope vaccine and immune receptor (TLR4) of the host were evaluated based on molecular dockings of the predicted 3D structures. Finally, in silico cloning was performed to construct the expression vaccine vector. Docking analysis showed that the vaccine/TLR4 complex took a stable form. Based on the predicted immunogenicity, physicochemical, and structural properties in silico, the vaccine candidate was expected to be appropriately expressed in bacterial expression systems and show the potential to induce a host immune response. This study proposes the experimental validation of the efficacy of the candidate vaccine construct against the two <i>Leishmania</i>. |
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| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-09T20:39:55Z |
| publishDate | 2022-10-01 |
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| spelling | doaj.art-78f2e5fffec1444b86e57658168ddc3e2023-11-23T22:59:55ZengMDPI AGBiology2079-77372022-10-011110146010.3390/biology11101460Design of a Chimeric Multi-Epitope Vaccine (CMEV) against Both <i>Leishmania martiniquensis</i> and <i>Leishmania orientalis</i> Parasites Using Immunoinformatic ApproachesKentaro Imaizumi0Thararat Phurahong1Suradej Siripattanapipong2Kiattawee Choowongkomon3Saovanee Leelayoova4Mathirut Mungthin5Teerasak E-kobon6Sasimanas Unajak7Department of Biochemistry, Faculty of Science, Kasetsart University, 50 Ngam Wong Wan, Chatuchak, Bangkok 10900, ThailandDepartment of Biochemistry, Faculty of Science, Kasetsart University, 50 Ngam Wong Wan, Chatuchak, Bangkok 10900, ThailandDepartment of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, ThailandDepartment of Biochemistry, Faculty of Science, Kasetsart University, 50 Ngam Wong Wan, Chatuchak, Bangkok 10900, ThailandDepartment of Parasitology, Phramongkutklao College of Medicine, Bangkok 10400, ThailandDepartment of Parasitology, Phramongkutklao College of Medicine, Bangkok 10400, ThailandDepartment of Genetics, Faculty of Science, Kasetsart University, 50 Ngam Wong Wan, Chatuchak, Bangkok 10900, ThailandDepartment of Biochemistry, Faculty of Science, Kasetsart University, 50 Ngam Wong Wan, Chatuchak, Bangkok 10900, ThailandLeishmaniasis is a parasitic disease caused by protozoan flagellates of the genus <i>Leishmania</i>. Recently, <i>Leishmania martiniquensis</i> and <i>Leishmania orientalis</i>, emerging species of <i>Leishmania</i>, were isolated from patients in Thailand. Development of the vaccine is demanded; however, genetic differences between the two species make it difficult to design a vaccine that is effective for both species. In this study, we applied immuno-informatic approaches to design a chimeric multi-epitope vaccine (CMEV) against both <i>L. martiniquensis</i> and <i>L. orientalis</i>. We identified seven helper T lymphocyte (HTL) epitopes, sixteen cytotoxic T lymphocyte (CTL) epitopes, and eleven B-cell epitopes from sixteen conserved antigenic proteins found in both species. All these epitopes were joined together, and to further enhance immunogenicity, protein and peptides adjuvant were also added at the N-terminal of the molecule by using specific linkers. The candidate CMEV was subsequently analyzed from the perspectives of the antigenicity, allergenicity, and physiochemical properties. The interaction of the designed multi-epitope vaccine and immune receptor (TLR4) of the host were evaluated based on molecular dockings of the predicted 3D structures. Finally, in silico cloning was performed to construct the expression vaccine vector. Docking analysis showed that the vaccine/TLR4 complex took a stable form. Based on the predicted immunogenicity, physicochemical, and structural properties in silico, the vaccine candidate was expected to be appropriately expressed in bacterial expression systems and show the potential to induce a host immune response. This study proposes the experimental validation of the efficacy of the candidate vaccine construct against the two <i>Leishmania</i>.https://www.mdpi.com/2079-7737/11/10/1460leishmaniasischimeric multi-epitope vaccineimmuno-informaticsin silico vaccine designreverse vaccinologyneglected tropical diseases |
| spellingShingle | Kentaro Imaizumi Thararat Phurahong Suradej Siripattanapipong Kiattawee Choowongkomon Saovanee Leelayoova Mathirut Mungthin Teerasak E-kobon Sasimanas Unajak Design of a Chimeric Multi-Epitope Vaccine (CMEV) against Both <i>Leishmania martiniquensis</i> and <i>Leishmania orientalis</i> Parasites Using Immunoinformatic Approaches Biology leishmaniasis chimeric multi-epitope vaccine immuno-informatics in silico vaccine design reverse vaccinology neglected tropical diseases |
| title | Design of a Chimeric Multi-Epitope Vaccine (CMEV) against Both <i>Leishmania martiniquensis</i> and <i>Leishmania orientalis</i> Parasites Using Immunoinformatic Approaches |
| title_full | Design of a Chimeric Multi-Epitope Vaccine (CMEV) against Both <i>Leishmania martiniquensis</i> and <i>Leishmania orientalis</i> Parasites Using Immunoinformatic Approaches |
| title_fullStr | Design of a Chimeric Multi-Epitope Vaccine (CMEV) against Both <i>Leishmania martiniquensis</i> and <i>Leishmania orientalis</i> Parasites Using Immunoinformatic Approaches |
| title_full_unstemmed | Design of a Chimeric Multi-Epitope Vaccine (CMEV) against Both <i>Leishmania martiniquensis</i> and <i>Leishmania orientalis</i> Parasites Using Immunoinformatic Approaches |
| title_short | Design of a Chimeric Multi-Epitope Vaccine (CMEV) against Both <i>Leishmania martiniquensis</i> and <i>Leishmania orientalis</i> Parasites Using Immunoinformatic Approaches |
| title_sort | design of a chimeric multi epitope vaccine cmev against both i leishmania martiniquensis i and i leishmania orientalis i parasites using immunoinformatic approaches |
| topic | leishmaniasis chimeric multi-epitope vaccine immuno-informatics in silico vaccine design reverse vaccinology neglected tropical diseases |
| url | https://www.mdpi.com/2079-7737/11/10/1460 |
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