MK2 drives progression of pancreas and colon cancers by suppressing CD8+ T cell cytotoxic function and is a potential immunotherapy target
BackgroundImmune cell composition is a critical and dynamic component of the tumor microenvironment, which has an impact on immunosuppression and progression of cancer. T cells, especially CD8+ T cells, are one of the major immune cell types responsible for tumor cell killing employing receptor-liga...
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1212100/full |
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| author | Damian Jacenik Eric J. Lebish Ellen J. Beswick Ellen J. Beswick |
| author_facet | Damian Jacenik Eric J. Lebish Ellen J. Beswick Ellen J. Beswick |
| author_sort | Damian Jacenik |
| collection | DOAJ |
| description | BackgroundImmune cell composition is a critical and dynamic component of the tumor microenvironment, which has an impact on immunosuppression and progression of cancer. T cells, especially CD8+ T cells, are one of the major immune cell types responsible for tumor cell killing employing receptor-ligand mediated apoptosis and/or releasing lytic granules among others. Accumulating evidence highlighted that adoptive transfer of activated and/or modified immune cells can enhance anti-tumorigenic immune responses and serve as promising therapy approach for patients with cancers. The mitogen-activated protein kinase-activated protein kinase 2 (MK2) is a serine/threonine protein kinase, which controls production and secretion of numerous pro-inflammatory cytokines and chemokines involved in tumorigenesis. However, limited efforts have been made to learn how MK2 may affects CD8+ T cell action and function in the tumor microenvironment especially in gastrointestinal cancers.MethodsTo explore the therapeutic potential of MK2 in the immune response mediated by CD8+ T cells, RAG1 knockout mice with PK5L1940 and BRAF cells-derived allograft tumors were treated with WT or MK2 knockout CD8+ T cells. The phenotype of CD8+ T cells with MK2 depletion were evaluated in vitro. Immunofluorescence staining, real-time PCR and multiplex analysis were utilized to estimate the expression of apoptotic and lytic factors.ResultsHere, we show that CD8+ T cells with MK2 depletion prevent gastrointestinal cancer growth, which is accompanied by enhanced expression and secretion of factors related to apoptosis. Moreover, using in vitro and in vivo approaches, we found that depletion of MK2 lead to hyperactivation of CD8+ T cells and enhanced anti-tumor immunity.ConclusionOverall, we documented that MK2 drives the progression of gastrointestinal cancers and prevents immune response generated by CD8+ T cells suggesting potential implications of MK2 in the immunotherapy of gastrointestinal cancers. |
| first_indexed | 2024-03-13T04:04:18Z |
| format | Article |
| id | doaj.art-78f38482574642fbaf402f2dab6af8fb |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-03-13T04:04:18Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-78f38482574642fbaf402f2dab6af8fb2023-06-21T09:56:24ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-06-011410.3389/fimmu.2023.12121001212100MK2 drives progression of pancreas and colon cancers by suppressing CD8+ T cell cytotoxic function and is a potential immunotherapy targetDamian Jacenik0Eric J. Lebish1Ellen J. Beswick2Ellen J. Beswick3Department of Cytobiochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, PolandDivision of Gastroenterology, Department of Internal Medicine, University of Utah, Salt Lake City, UT, United StatesDivision of Gastroenterology, Department of Internal Medicine, University of Utah, Salt Lake City, UT, United StatesDivision of Digestive Diseases and Nutrition, Department of Internal Medicine, University of Kentucky, Lexington, KY, United StatesBackgroundImmune cell composition is a critical and dynamic component of the tumor microenvironment, which has an impact on immunosuppression and progression of cancer. T cells, especially CD8+ T cells, are one of the major immune cell types responsible for tumor cell killing employing receptor-ligand mediated apoptosis and/or releasing lytic granules among others. Accumulating evidence highlighted that adoptive transfer of activated and/or modified immune cells can enhance anti-tumorigenic immune responses and serve as promising therapy approach for patients with cancers. The mitogen-activated protein kinase-activated protein kinase 2 (MK2) is a serine/threonine protein kinase, which controls production and secretion of numerous pro-inflammatory cytokines and chemokines involved in tumorigenesis. However, limited efforts have been made to learn how MK2 may affects CD8+ T cell action and function in the tumor microenvironment especially in gastrointestinal cancers.MethodsTo explore the therapeutic potential of MK2 in the immune response mediated by CD8+ T cells, RAG1 knockout mice with PK5L1940 and BRAF cells-derived allograft tumors were treated with WT or MK2 knockout CD8+ T cells. The phenotype of CD8+ T cells with MK2 depletion were evaluated in vitro. Immunofluorescence staining, real-time PCR and multiplex analysis were utilized to estimate the expression of apoptotic and lytic factors.ResultsHere, we show that CD8+ T cells with MK2 depletion prevent gastrointestinal cancer growth, which is accompanied by enhanced expression and secretion of factors related to apoptosis. Moreover, using in vitro and in vivo approaches, we found that depletion of MK2 lead to hyperactivation of CD8+ T cells and enhanced anti-tumor immunity.ConclusionOverall, we documented that MK2 drives the progression of gastrointestinal cancers and prevents immune response generated by CD8+ T cells suggesting potential implications of MK2 in the immunotherapy of gastrointestinal cancers.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1212100/fullmitogen-activated protein kinase-activated protein kinase 2MAPKAPK2MK2cytotoxic T cellCD8+ T cellpancreas cancer |
| spellingShingle | Damian Jacenik Eric J. Lebish Ellen J. Beswick Ellen J. Beswick MK2 drives progression of pancreas and colon cancers by suppressing CD8+ T cell cytotoxic function and is a potential immunotherapy target Frontiers in Immunology mitogen-activated protein kinase-activated protein kinase 2 MAPKAPK2 MK2 cytotoxic T cell CD8+ T cell pancreas cancer |
| title | MK2 drives progression of pancreas and colon cancers by suppressing CD8+ T cell cytotoxic function and is a potential immunotherapy target |
| title_full | MK2 drives progression of pancreas and colon cancers by suppressing CD8+ T cell cytotoxic function and is a potential immunotherapy target |
| title_fullStr | MK2 drives progression of pancreas and colon cancers by suppressing CD8+ T cell cytotoxic function and is a potential immunotherapy target |
| title_full_unstemmed | MK2 drives progression of pancreas and colon cancers by suppressing CD8+ T cell cytotoxic function and is a potential immunotherapy target |
| title_short | MK2 drives progression of pancreas and colon cancers by suppressing CD8+ T cell cytotoxic function and is a potential immunotherapy target |
| title_sort | mk2 drives progression of pancreas and colon cancers by suppressing cd8 t cell cytotoxic function and is a potential immunotherapy target |
| topic | mitogen-activated protein kinase-activated protein kinase 2 MAPKAPK2 MK2 cytotoxic T cell CD8+ T cell pancreas cancer |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1212100/full |
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