The influence of alpha-lipoic acid on ovarian follicle growth in induced aging mice
The ovary contains follicles at various developmental stages. The present study aimed to investigate the antioxidant efficiency of alpha-lipoic acid (α-LA) on follicle growth in induced aging ovaries. Juvenile female mice (n=24) were allocated into four groups (n=6, each); the control group received...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | Arabic |
| Published: |
University of Mosul, College of Veterinary Medicine
2023-04-01
|
| Series: | Iraqi Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | https://www.vetmedmosul.com/article_177310_69990f0790d6a5ab306a2b9365da2770.pdf |
| _version_ | 1827975400039383040 |
|---|---|
| author | Isam B. Sharum Enas O. Hussien Fadwa Tawfeeq |
| author_facet | Isam B. Sharum Enas O. Hussien Fadwa Tawfeeq |
| author_sort | Isam B. Sharum |
| collection | DOAJ |
| description | The ovary contains follicles at various developmental stages. The present study aimed to investigate the antioxidant efficiency of alpha-lipoic acid (α-LA) on follicle growth in induced aging ovaries. Juvenile female mice (n=24) were allocated into four groups (n=6, each); the control group received distilled water, and the induced aging group (T1) received D-galactose (300 mg/kg). The co-administrated group (T2) was treated with α-LA (300 mg/kg) and D-galactose (100 mg/kg), while the fourth group (T3) was treated with α-LA (100 mg/kg). At the end of treatments (8 weeks), animals were sacrificed, and ovaries were processed for hematoxylin and eosin staining and immunostaining for proliferating cell nuclear antigen (PCNA). PCNA was detectable in oocytes but only in granulosa cells of activated follicles. The D-galactose treatment successfully induced ovarian aging as the proportion of primordial and growing follicles was significantly reduced accompanied by massively increased atretic follicles. Additionally, only numerous PCNA positively stained follicles were recognized. The co-administrated α-LA moderately rescued the characteristics of ovarian aging. Mice treated with α-LA demonstrated a substantial increase in the population of atretic follicles, antral follicles, and PCNA positively stained follicles. There was no change in oocyte size at any follicle growth stage among groups. In conclusion, α-LA moderately rescued the detrimental impacts of induced ovarian aging. For the first time, the expression of PCNA was linked with ovarian aging, where PCNA-staining has been recognized as a valuable tool to evaluate the proliferative activity of the granulosa cells. |
| first_indexed | 2024-04-09T20:11:01Z |
| format | Article |
| id | doaj.art-78f50019a0ea4c2893ddf0af7138e0d7 |
| institution | Directory Open Access Journal |
| issn | 1607-3894 2071-1255 |
| language | Arabic |
| last_indexed | 2024-04-09T20:11:01Z |
| publishDate | 2023-04-01 |
| publisher | University of Mosul, College of Veterinary Medicine |
| record_format | Article |
| series | Iraqi Journal of Veterinary Sciences |
| spelling | doaj.art-78f50019a0ea4c2893ddf0af7138e0d72023-03-31T23:07:07ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552023-04-0137237538210.33899/ijvs.2022.134092.2342177310The influence of alpha-lipoic acid on ovarian follicle growth in induced aging miceIsam B. Sharum0Enas O. Hussien1Fadwa Tawfeeq2Department of Surgery and Theriogenology, College of Veterinary Medicine, University of Mosul, Mosul, IraqDepartment of Physiology, Biochemistry, and Pharmacology, College of Veterinary Medicine, University of Mosul, Mosul, IraqDepartment of Physiology, Biochemistry and Pharmacology College of Veterinary Medicine- University of Mosul; Mosul, IraqThe ovary contains follicles at various developmental stages. The present study aimed to investigate the antioxidant efficiency of alpha-lipoic acid (α-LA) on follicle growth in induced aging ovaries. Juvenile female mice (n=24) were allocated into four groups (n=6, each); the control group received distilled water, and the induced aging group (T1) received D-galactose (300 mg/kg). The co-administrated group (T2) was treated with α-LA (300 mg/kg) and D-galactose (100 mg/kg), while the fourth group (T3) was treated with α-LA (100 mg/kg). At the end of treatments (8 weeks), animals were sacrificed, and ovaries were processed for hematoxylin and eosin staining and immunostaining for proliferating cell nuclear antigen (PCNA). PCNA was detectable in oocytes but only in granulosa cells of activated follicles. The D-galactose treatment successfully induced ovarian aging as the proportion of primordial and growing follicles was significantly reduced accompanied by massively increased atretic follicles. Additionally, only numerous PCNA positively stained follicles were recognized. The co-administrated α-LA moderately rescued the characteristics of ovarian aging. Mice treated with α-LA demonstrated a substantial increase in the population of atretic follicles, antral follicles, and PCNA positively stained follicles. There was no change in oocyte size at any follicle growth stage among groups. In conclusion, α-LA moderately rescued the detrimental impacts of induced ovarian aging. For the first time, the expression of PCNA was linked with ovarian aging, where PCNA-staining has been recognized as a valuable tool to evaluate the proliferative activity of the granulosa cells.https://www.vetmedmosul.com/article_177310_69990f0790d6a5ab306a2b9365da2770.pdfalpha-lipoic acidinduced ovarian agingpcna immunoreactivity |
| spellingShingle | Isam B. Sharum Enas O. Hussien Fadwa Tawfeeq The influence of alpha-lipoic acid on ovarian follicle growth in induced aging mice Iraqi Journal of Veterinary Sciences alpha-lipoic acid induced ovarian aging pcna immunoreactivity |
| title | The influence of alpha-lipoic acid on ovarian follicle growth in induced aging mice |
| title_full | The influence of alpha-lipoic acid on ovarian follicle growth in induced aging mice |
| title_fullStr | The influence of alpha-lipoic acid on ovarian follicle growth in induced aging mice |
| title_full_unstemmed | The influence of alpha-lipoic acid on ovarian follicle growth in induced aging mice |
| title_short | The influence of alpha-lipoic acid on ovarian follicle growth in induced aging mice |
| title_sort | influence of alpha lipoic acid on ovarian follicle growth in induced aging mice |
| topic | alpha-lipoic acid induced ovarian aging pcna immunoreactivity |
| url | https://www.vetmedmosul.com/article_177310_69990f0790d6a5ab306a2b9365da2770.pdf |
| work_keys_str_mv | AT isambsharum theinfluenceofalphalipoicacidonovarianfolliclegrowthininducedagingmice AT enasohussien theinfluenceofalphalipoicacidonovarianfolliclegrowthininducedagingmice AT fadwatawfeeq theinfluenceofalphalipoicacidonovarianfolliclegrowthininducedagingmice AT isambsharum influenceofalphalipoicacidonovarianfolliclegrowthininducedagingmice AT enasohussien influenceofalphalipoicacidonovarianfolliclegrowthininducedagingmice AT fadwatawfeeq influenceofalphalipoicacidonovarianfolliclegrowthininducedagingmice |