Probiotic characterization of Enterococcus faecium por1: Cloning, over expression of Enterocin-A and evaluation of antibacterial, anti-cancer properties
New probiotic strain-por1 showed anti-microbial activity against a wide variety of pathogens was isolated from intestinal food content of porcupine and identified as an Enterococcus faecium (por1) by 16S rRNA sequencing. The strain E. faecium por1 was able to survive in different bile and acidic con...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2017-11-01
|
| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464617305558 |
| _version_ | 1818563920388947968 |
|---|---|
| author | Dasari Ankaiah Palanichamy Esakkiraj Venkatesh Perumal Repally Ayyanna Arul Venkatesan |
| author_facet | Dasari Ankaiah Palanichamy Esakkiraj Venkatesh Perumal Repally Ayyanna Arul Venkatesan |
| author_sort | Dasari Ankaiah |
| collection | DOAJ |
| description | New probiotic strain-por1 showed anti-microbial activity against a wide variety of pathogens was isolated from intestinal food content of porcupine and identified as an Enterococcus faecium (por1) by 16S rRNA sequencing. The strain E. faecium por1 was able to survive in different bile and acidic conditions, non-haemolytic. The genes responsible for virulence and subsequent pathogenicity such as cylA, cylB, esp, ace and asa1 were absent in the strain E. faecium por1 and a gene efaAfm responsible for cell wall adherence was present. Three different bacteriocins genes such as ent-A, ent-B and ent-P were identified in E. faecium por1 and in this study one of the gene ent-A was successfully cloned and overexpressed in E. coli BL21 (DE3). Further, the expressed and purified bacteriocin enterocin-A showed effective anti-bacterial and anti-biofilm activity against food born as well as human bacterial pathogens. This is the first report that a bacteriocin enterocin-A exhibited anticancer activity against human colon, gastric (HT-29, Caco-2 and AGS), cervical (HeLa) cancer cells and this bacteriocin enterocin-A was displayed no cytotoxicity toward normal intestinal epithelial cells (INT-407). |
| first_indexed | 2024-12-14T01:22:38Z |
| format | Article |
| id | doaj.art-78f916dbb3fc4e8792e5f88c5a4ce168 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-14T01:22:38Z |
| publishDate | 2017-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-78f916dbb3fc4e8792e5f88c5a4ce1682022-12-21T23:22:20ZengElsevierJournal of Functional Foods1756-46462017-11-0138280292Probiotic characterization of Enterococcus faecium por1: Cloning, over expression of Enterocin-A and evaluation of antibacterial, anti-cancer propertiesDasari Ankaiah0Palanichamy Esakkiraj1Venkatesh Perumal2Repally Ayyanna3Arul Venkatesan4Department of Biotechnology, School of Life Sciences, Pondicherry University, Puducherry 605014, IndiaDepartment of Biotechnology, School of Life Sciences, Pondicherry University, Puducherry 605014, IndiaDepartment of Biotechnology, School of Life Sciences, Pondicherry University, Puducherry 605014, IndiaDepartment of Biotechnology, School of Life Sciences, Pondicherry University, Puducherry 605014, IndiaCorresponding author.; Department of Biotechnology, School of Life Sciences, Pondicherry University, Puducherry 605014, IndiaNew probiotic strain-por1 showed anti-microbial activity against a wide variety of pathogens was isolated from intestinal food content of porcupine and identified as an Enterococcus faecium (por1) by 16S rRNA sequencing. The strain E. faecium por1 was able to survive in different bile and acidic conditions, non-haemolytic. The genes responsible for virulence and subsequent pathogenicity such as cylA, cylB, esp, ace and asa1 were absent in the strain E. faecium por1 and a gene efaAfm responsible for cell wall adherence was present. Three different bacteriocins genes such as ent-A, ent-B and ent-P were identified in E. faecium por1 and in this study one of the gene ent-A was successfully cloned and overexpressed in E. coli BL21 (DE3). Further, the expressed and purified bacteriocin enterocin-A showed effective anti-bacterial and anti-biofilm activity against food born as well as human bacterial pathogens. This is the first report that a bacteriocin enterocin-A exhibited anticancer activity against human colon, gastric (HT-29, Caco-2 and AGS), cervical (HeLa) cancer cells and this bacteriocin enterocin-A was displayed no cytotoxicity toward normal intestinal epithelial cells (INT-407).http://www.sciencedirect.com/science/article/pii/S1756464617305558E. faecium por1Enterocin-ACloningOverexpressionAnti-biofilm activityAnti-cancer activity |
| spellingShingle | Dasari Ankaiah Palanichamy Esakkiraj Venkatesh Perumal Repally Ayyanna Arul Venkatesan Probiotic characterization of Enterococcus faecium por1: Cloning, over expression of Enterocin-A and evaluation of antibacterial, anti-cancer properties Journal of Functional Foods E. faecium por1 Enterocin-A Cloning Overexpression Anti-biofilm activity Anti-cancer activity |
| title | Probiotic characterization of Enterococcus faecium por1: Cloning, over expression of Enterocin-A and evaluation of antibacterial, anti-cancer properties |
| title_full | Probiotic characterization of Enterococcus faecium por1: Cloning, over expression of Enterocin-A and evaluation of antibacterial, anti-cancer properties |
| title_fullStr | Probiotic characterization of Enterococcus faecium por1: Cloning, over expression of Enterocin-A and evaluation of antibacterial, anti-cancer properties |
| title_full_unstemmed | Probiotic characterization of Enterococcus faecium por1: Cloning, over expression of Enterocin-A and evaluation of antibacterial, anti-cancer properties |
| title_short | Probiotic characterization of Enterococcus faecium por1: Cloning, over expression of Enterocin-A and evaluation of antibacterial, anti-cancer properties |
| title_sort | probiotic characterization of enterococcus faecium por1 cloning over expression of enterocin a and evaluation of antibacterial anti cancer properties |
| topic | E. faecium por1 Enterocin-A Cloning Overexpression Anti-biofilm activity Anti-cancer activity |
| url | http://www.sciencedirect.com/science/article/pii/S1756464617305558 |
| work_keys_str_mv | AT dasariankaiah probioticcharacterizationofenterococcusfaeciumpor1cloningoverexpressionofenterocinaandevaluationofantibacterialanticancerproperties AT palanichamyesakkiraj probioticcharacterizationofenterococcusfaeciumpor1cloningoverexpressionofenterocinaandevaluationofantibacterialanticancerproperties AT venkateshperumal probioticcharacterizationofenterococcusfaeciumpor1cloningoverexpressionofenterocinaandevaluationofantibacterialanticancerproperties AT repallyayyanna probioticcharacterizationofenterococcusfaeciumpor1cloningoverexpressionofenterocinaandevaluationofantibacterialanticancerproperties AT arulvenkatesan probioticcharacterizationofenterococcusfaeciumpor1cloningoverexpressionofenterocinaandevaluationofantibacterialanticancerproperties |