Biochemical association of metabolic profile and microbiome in chronic pressure ulcer wounds.
Chronic, non-healing wounds contribute significantly to the suffering of patients with co-morbidities in the clinical population with mild to severely compromised immune systems. Normal wound healing proceeds through a well-described process. However, in chronic wounds this process seems to become d...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4433261?pdf=render |
| _version_ | 1818034883596910592 |
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| author | Mary Cloud B Ammons Kathryn Morrissey Brian P Tripet James T Van Leuven Anne Han Gerald S Lazarus Jonathan M Zenilman Philip S Stewart Garth A James Valérie Copié |
| author_facet | Mary Cloud B Ammons Kathryn Morrissey Brian P Tripet James T Van Leuven Anne Han Gerald S Lazarus Jonathan M Zenilman Philip S Stewart Garth A James Valérie Copié |
| author_sort | Mary Cloud B Ammons |
| collection | DOAJ |
| description | Chronic, non-healing wounds contribute significantly to the suffering of patients with co-morbidities in the clinical population with mild to severely compromised immune systems. Normal wound healing proceeds through a well-described process. However, in chronic wounds this process seems to become dysregulated at the transition between resolution of inflammation and re-epithelialization. Bioburden in the form of colonizing bacteria is a major contributor to the delayed headlining in chronic wounds such as pressure ulcers. However how the microbiome influences the wound metabolic landscape is unknown. Here, we have used a Systems Biology approach to determine the biochemical associations between the taxonomic and metabolomic profiles of wounds colonized by bacteria. Pressure ulcer biopsies were harvested from primary chronic wounds and bisected into top and bottom sections prior to analysis of microbiome by pyrosequencing and analysis of metabolome using 1H nuclear magnetic resonance (NMR) spectroscopy. Bacterial taxonomy revealed that wounds were colonized predominantly by three main phyla, but differed significantly at the genus level. While taxonomic profiles demonstrated significant variability between wounds, metabolic profiles shared significant similarity based on the depth of the wound biopsy. Biochemical association between taxonomy and metabolic landscape indicated significant wound-to-wound similarity in metabolite enrichment sets and metabolic pathway impacts, especially with regard to amino acid metabolism. To our knowledge, this is the first demonstration of a statistically robust correlation between bacterial colonization and metabolic landscape within the chronic wound environment. |
| first_indexed | 2024-12-10T06:46:14Z |
| format | Article |
| id | doaj.art-78fa39088d6c4e20a8804ebe2f61f7cc |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T06:46:14Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-78fa39088d6c4e20a8804ebe2f61f7cc2022-12-22T01:58:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01105e012673510.1371/journal.pone.0126735Biochemical association of metabolic profile and microbiome in chronic pressure ulcer wounds.Mary Cloud B AmmonsKathryn MorrisseyBrian P TripetJames T Van LeuvenAnne HanGerald S LazarusJonathan M ZenilmanPhilip S StewartGarth A JamesValérie CopiéChronic, non-healing wounds contribute significantly to the suffering of patients with co-morbidities in the clinical population with mild to severely compromised immune systems. Normal wound healing proceeds through a well-described process. However, in chronic wounds this process seems to become dysregulated at the transition between resolution of inflammation and re-epithelialization. Bioburden in the form of colonizing bacteria is a major contributor to the delayed headlining in chronic wounds such as pressure ulcers. However how the microbiome influences the wound metabolic landscape is unknown. Here, we have used a Systems Biology approach to determine the biochemical associations between the taxonomic and metabolomic profiles of wounds colonized by bacteria. Pressure ulcer biopsies were harvested from primary chronic wounds and bisected into top and bottom sections prior to analysis of microbiome by pyrosequencing and analysis of metabolome using 1H nuclear magnetic resonance (NMR) spectroscopy. Bacterial taxonomy revealed that wounds were colonized predominantly by three main phyla, but differed significantly at the genus level. While taxonomic profiles demonstrated significant variability between wounds, metabolic profiles shared significant similarity based on the depth of the wound biopsy. Biochemical association between taxonomy and metabolic landscape indicated significant wound-to-wound similarity in metabolite enrichment sets and metabolic pathway impacts, especially with regard to amino acid metabolism. To our knowledge, this is the first demonstration of a statistically robust correlation between bacterial colonization and metabolic landscape within the chronic wound environment.http://europepmc.org/articles/PMC4433261?pdf=render |
| spellingShingle | Mary Cloud B Ammons Kathryn Morrissey Brian P Tripet James T Van Leuven Anne Han Gerald S Lazarus Jonathan M Zenilman Philip S Stewart Garth A James Valérie Copié Biochemical association of metabolic profile and microbiome in chronic pressure ulcer wounds. PLoS ONE |
| title | Biochemical association of metabolic profile and microbiome in chronic pressure ulcer wounds. |
| title_full | Biochemical association of metabolic profile and microbiome in chronic pressure ulcer wounds. |
| title_fullStr | Biochemical association of metabolic profile and microbiome in chronic pressure ulcer wounds. |
| title_full_unstemmed | Biochemical association of metabolic profile and microbiome in chronic pressure ulcer wounds. |
| title_short | Biochemical association of metabolic profile and microbiome in chronic pressure ulcer wounds. |
| title_sort | biochemical association of metabolic profile and microbiome in chronic pressure ulcer wounds |
| url | http://europepmc.org/articles/PMC4433261?pdf=render |
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