Standardization of flow cytometry and cell sorting to enable a transcriptomic analysis in a multi-site sarcoidosis study.

Standardization of flow cytometry and cell sorting to enable a transcriptomic analysis in a multi-site sarcoidosis study.

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The contribution and regulation of various CD4+ T cell lineages that occur with remitting vs progressive courses in sarcoidosis are poorly understood. We developed a multiparameter flow cytometry panel to sort these CD4+ T cell lineages followed by measurement of their functional potential using RNA...

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Bibliographic Details
Main Authors: Roman E Magallon, Laura D Harmacek, Nicholas K Arger, Pineet Grewal, Linda Powers, Brenda R Werner, Briana Q Barkes, Li Li, Kristyn MacPhail, May Gillespie, Elizabeth K White, Sarah E Collins, Talyor Brown, Jessica Cardenas, Edward S Chen, Lisa A Maier, Sonia M Leach, Nabeel Y Hamzeh, Laura L Koth, Brian P O'Connor
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2023-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0281210

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