Expression of psbA1 gene in Synechocystis sp. PCC 6803 is influenced by CO2
In almost all cyanobacteria a small gene family encodes the photosystem II reaction center D1 proteins that play vital roles in the cell. Recently, several types of this protein were functionally characterised and the conditions for their transcript regulation were identified. One of the D1-encoding...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2017-05-01
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| Series: | Open Life Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/biol-2017-0018 |
| _version_ | 1818652911012413440 |
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| author | Chiş Ciprian Carmel Dalton Chiş luliana Ardelean Aurel Dragos Nicolae Sicora Cosmin |
| author_facet | Chiş Ciprian Carmel Dalton Chiş luliana Ardelean Aurel Dragos Nicolae Sicora Cosmin |
| author_sort | Chiş Ciprian |
| collection | DOAJ |
| description | In almost all cyanobacteria a small gene family encodes the photosystem II reaction center D1 proteins that play vital roles in the cell. Recently, several types of this protein were functionally characterised and the conditions for their transcript regulation were identified. One of the D1-encoding genes previously believed to be silent is induced by microaerobic conditions. This gene was first described in Synechocystis sp. PCC 6803 as psbA1 encoding the D1 isoform. When Synechocystis cells are shifted from high to ambient level CO2 we recorded an increase in gene expression, similar, but to a lesser extent, to microaerobic conditions. When synthetic air is used to remove the ambient CO2, the induction of the gene is absent. We documented for the first time that expression of a psbA gene is regulated by the inorganic carbon status of the cell. Our conclusion is that both CO2 and microaerobic conditions are independently influencing the expression of psbA1 gene in Synechocystis sp. PCC 6803. Hence, it is crucial to understand the mechanisms of regulation and function of D1 proteins as it could be used for future bio-technological applications as a virtual tool-box for modulating the function of PSII. |
| first_indexed | 2024-12-17T02:29:31Z |
| format | Article |
| id | doaj.art-78fc8ed3d4524bbf83f9b18be67e6151 |
| institution | Directory Open Access Journal |
| issn | 2391-5412 |
| language | English |
| last_indexed | 2024-12-17T02:29:31Z |
| publishDate | 2017-05-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Life Sciences |
| spelling | doaj.art-78fc8ed3d4524bbf83f9b18be67e61512022-12-21T22:07:00ZengDe GruyterOpen Life Sciences2391-54122017-05-0112115616110.1515/biol-2017-0018biol-2017-0018Expression of psbA1 gene in Synechocystis sp. PCC 6803 is influenced by CO2Chiş Ciprian0Carmel Dalton1Chiş luliana2Ardelean Aurel3Dragos Nicolae4Sicora Cosmin5Biological Research Centre, Jibou, RomaniaBiological Research Centre, Jibou, RomaniaBiological Research Centre, Jibou, RomaniaWestern University ”Vasile Goldis”, Arad, RomaniaBabeş-Bolyai University, Cluj-Napoca, RomaniaBiological Research Centre, Jibou, RomaniaIn almost all cyanobacteria a small gene family encodes the photosystem II reaction center D1 proteins that play vital roles in the cell. Recently, several types of this protein were functionally characterised and the conditions for their transcript regulation were identified. One of the D1-encoding genes previously believed to be silent is induced by microaerobic conditions. This gene was first described in Synechocystis sp. PCC 6803 as psbA1 encoding the D1 isoform. When Synechocystis cells are shifted from high to ambient level CO2 we recorded an increase in gene expression, similar, but to a lesser extent, to microaerobic conditions. When synthetic air is used to remove the ambient CO2, the induction of the gene is absent. We documented for the first time that expression of a psbA gene is regulated by the inorganic carbon status of the cell. Our conclusion is that both CO2 and microaerobic conditions are independently influencing the expression of psbA1 gene in Synechocystis sp. PCC 6803. Hence, it is crucial to understand the mechanisms of regulation and function of D1 proteins as it could be used for future bio-technological applications as a virtual tool-box for modulating the function of PSII.https://doi.org/10.1515/biol-2017-0018synechocystisphotosystem iipsba1d1photosynthesiscarbon dioxidemicroaerobicqrt-rt-pcrchlorophyllfluorescence |
| spellingShingle | Chiş Ciprian Carmel Dalton Chiş luliana Ardelean Aurel Dragos Nicolae Sicora Cosmin Expression of psbA1 gene in Synechocystis sp. PCC 6803 is influenced by CO2 Open Life Sciences synechocystis photosystem ii psba1 d1 photosynthesis carbon dioxide microaerobic qrt-rt-pcr chlorophyll fluorescence |
| title | Expression of psbA1 gene in Synechocystis sp. PCC 6803 is influenced by CO2 |
| title_full | Expression of psbA1 gene in Synechocystis sp. PCC 6803 is influenced by CO2 |
| title_fullStr | Expression of psbA1 gene in Synechocystis sp. PCC 6803 is influenced by CO2 |
| title_full_unstemmed | Expression of psbA1 gene in Synechocystis sp. PCC 6803 is influenced by CO2 |
| title_short | Expression of psbA1 gene in Synechocystis sp. PCC 6803 is influenced by CO2 |
| title_sort | expression of psba1 gene in synechocystis sp pcc 6803 is influenced by co2 |
| topic | synechocystis photosystem ii psba1 d1 photosynthesis carbon dioxide microaerobic qrt-rt-pcr chlorophyll fluorescence |
| url | https://doi.org/10.1515/biol-2017-0018 |
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