Cannabichromene Induces Neuronal Differentiation in NSC-34 Cells: Insights from Transcriptomic Analysis
Phytocannabinoids, with their variety of beneficial effects, represent a valid group of substances that could be employed as neurogenesis-enhancers or neuronal differentiation inducers. We focused our attention on the neuronal-related potential of cannabichromene (CBC) when administered to undiffere...
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MDPI AG
2023-03-01
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| Online Access: | https://www.mdpi.com/2075-1729/13/3/742 |
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| author | Andrea Valeri Luigi Chiricosta Simone D’Angiolini Federica Pollastro Stefano Salamone Emanuela Mazzon |
| author_facet | Andrea Valeri Luigi Chiricosta Simone D’Angiolini Federica Pollastro Stefano Salamone Emanuela Mazzon |
| author_sort | Andrea Valeri |
| collection | DOAJ |
| description | Phytocannabinoids, with their variety of beneficial effects, represent a valid group of substances that could be employed as neurogenesis-enhancers or neuronal differentiation inducers. We focused our attention on the neuronal-related potential of cannabichromene (CBC) when administered to undifferentiated NSC-34 for 24 h. Transcriptomic analysis showed an upregulation of several neuronal markers, such as <i>Neurod1</i> and <i>Tubb3</i>, as well as indicators of neuronal differentiation process progression, such as <i>Pax6</i>. An in-depth investigation of the processes involved in neuronal differentiation indicates positive cytoskeleton remodeling by upregulation of <i>Cfl2</i> and <i>Tubg1</i>, and active differentiation-targeted transcriptional program, suggested by <i>Phox2b</i> and <i>Hes1.</i> After 48 h of treatment, the markers previously examined in the transcriptomic analysis are still overexpressed, like <i>Ache</i> and <i>Hes1</i>, indicating that the differentiation process is still in progress. The lack of GFAP protein suggests that no astroglial differentiation is taking place, and it is reasonable to indicate the neuronal one as the ongoing one. These results indicate CBC as a potential neuronal differentiation inducer for NSC-34 cells. |
| first_indexed | 2024-03-11T06:17:18Z |
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| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-11T06:17:18Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-78feeb7faaf14ce6b87b742cf649be412023-11-17T12:11:44ZengMDPI AGLife2075-17292023-03-0113374210.3390/life13030742Cannabichromene Induces Neuronal Differentiation in NSC-34 Cells: Insights from Transcriptomic AnalysisAndrea Valeri0Luigi Chiricosta1Simone D’Angiolini2Federica Pollastro3Stefano Salamone4Emanuela Mazzon5IRCCS Centro Neurolesi “Bonino-Pulejo”, Via Provinciale Palermo, Contrada Casazza, 98124 Messina, ItalyIRCCS Centro Neurolesi “Bonino-Pulejo”, Via Provinciale Palermo, Contrada Casazza, 98124 Messina, ItalyIRCCS Centro Neurolesi “Bonino-Pulejo”, Via Provinciale Palermo, Contrada Casazza, 98124 Messina, ItalyDepartment of Pharmaceutical Sciences, University of Eastern Piedmont, Largo Donegani 2, 28100 Novara, ItalyDepartment of Pharmaceutical Sciences, University of Eastern Piedmont, Largo Donegani 2, 28100 Novara, ItalyIRCCS Centro Neurolesi “Bonino-Pulejo”, Via Provinciale Palermo, Contrada Casazza, 98124 Messina, ItalyPhytocannabinoids, with their variety of beneficial effects, represent a valid group of substances that could be employed as neurogenesis-enhancers or neuronal differentiation inducers. We focused our attention on the neuronal-related potential of cannabichromene (CBC) when administered to undifferentiated NSC-34 for 24 h. Transcriptomic analysis showed an upregulation of several neuronal markers, such as <i>Neurod1</i> and <i>Tubb3</i>, as well as indicators of neuronal differentiation process progression, such as <i>Pax6</i>. An in-depth investigation of the processes involved in neuronal differentiation indicates positive cytoskeleton remodeling by upregulation of <i>Cfl2</i> and <i>Tubg1</i>, and active differentiation-targeted transcriptional program, suggested by <i>Phox2b</i> and <i>Hes1.</i> After 48 h of treatment, the markers previously examined in the transcriptomic analysis are still overexpressed, like <i>Ache</i> and <i>Hes1</i>, indicating that the differentiation process is still in progress. The lack of GFAP protein suggests that no astroglial differentiation is taking place, and it is reasonable to indicate the neuronal one as the ongoing one. These results indicate CBC as a potential neuronal differentiation inducer for NSC-34 cells.https://www.mdpi.com/2075-1729/13/3/742cannabichromeneneuronal differentiationNSC-34transcriptomic analysis |
| spellingShingle | Andrea Valeri Luigi Chiricosta Simone D’Angiolini Federica Pollastro Stefano Salamone Emanuela Mazzon Cannabichromene Induces Neuronal Differentiation in NSC-34 Cells: Insights from Transcriptomic Analysis Life cannabichromene neuronal differentiation NSC-34 transcriptomic analysis |
| title | Cannabichromene Induces Neuronal Differentiation in NSC-34 Cells: Insights from Transcriptomic Analysis |
| title_full | Cannabichromene Induces Neuronal Differentiation in NSC-34 Cells: Insights from Transcriptomic Analysis |
| title_fullStr | Cannabichromene Induces Neuronal Differentiation in NSC-34 Cells: Insights from Transcriptomic Analysis |
| title_full_unstemmed | Cannabichromene Induces Neuronal Differentiation in NSC-34 Cells: Insights from Transcriptomic Analysis |
| title_short | Cannabichromene Induces Neuronal Differentiation in NSC-34 Cells: Insights from Transcriptomic Analysis |
| title_sort | cannabichromene induces neuronal differentiation in nsc 34 cells insights from transcriptomic analysis |
| topic | cannabichromene neuronal differentiation NSC-34 transcriptomic analysis |
| url | https://www.mdpi.com/2075-1729/13/3/742 |
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