Small RNA perspective of physical exercise-related improvement of male reproductive dysfunction due to obesity
PurposeTo study whether physical exercise can effectively ameliorate obesity-induced abnormalities in male fertility and provide a new perspective on the role of small noncoding RNAs in spermatogenesis in obese male mice.MethodsIn this study, four-week-old C57/Bl6 male mice were randomly allocated t...
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| Format: | Article |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2022.1038449/full |
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| author | Tingting Lin Shuyu Zhang Yuchuan Zhou Ligang Wu Xinmei Liu Hefeng Huang Hefeng Huang Hefeng Huang Hefeng Huang |
| author_facet | Tingting Lin Shuyu Zhang Yuchuan Zhou Ligang Wu Xinmei Liu Hefeng Huang Hefeng Huang Hefeng Huang Hefeng Huang |
| author_sort | Tingting Lin |
| collection | DOAJ |
| description | PurposeTo study whether physical exercise can effectively ameliorate obesity-induced abnormalities in male fertility and provide a new perspective on the role of small noncoding RNAs in spermatogenesis in obese male mice.MethodsIn this study, four-week-old C57/Bl6 male mice were randomly allocated to receive a control diet, a high-fat diet or physical exercise intervention for 40 weeks. Purified round spermatids and spermatozoa were obtained after intervention. Sperm motility, concentration, the ability of the sperm to undergo capacitation and acrosome reaction were assessed. Small RNA sequencing was conducted on round spermatids and spermatozoa. The small noncoding RNAs expression pattern was systematically analyzed.ResultsThe spermatozoa concentration and percentage of motile spermatozoa, the capacitation and acrosome reaction, and the reproductive success rate, including mating success and pregnancy success, were decreased or delayed in the obesity group compared with controls. Physical exercise was able to restore the parameters to normal levels. Three microRNAs were consistently upregulated and 5 were downregulated in round spermatids and epididymal spermatozoa between the obesity and control groups.ConclusionsThis report provides evidence that the adverse effects of obesity could be offset after physical exercise. small noncoding RNAs, especially microRNAs in germ cells, may play an important role in the effects of obesity and physical exercise on spermatozoa. |
| first_indexed | 2024-04-12T04:02:53Z |
| format | Article |
| id | doaj.art-7902574d765047baad46ca4b495fabf9 |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-04-12T04:02:53Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-7902574d765047baad46ca4b495fabf92022-12-22T03:48:40ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922022-12-011310.3389/fendo.2022.10384491038449Small RNA perspective of physical exercise-related improvement of male reproductive dysfunction due to obesityTingting Lin0Shuyu Zhang1Yuchuan Zhou2Ligang Wu3Xinmei Liu4Hefeng Huang5Hefeng Huang6Hefeng Huang7Hefeng Huang8The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Embryo Original Diseases, Shanghai, ChinaThe International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Embryo Original Diseases, Shanghai, ChinaThe International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Embryo Original Diseases, Shanghai, ChinaState Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences–University of Chinese Academy of Sciences, Shanghai, ChinaObstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai, ChinaThe International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Embryo Original Diseases, Shanghai, ChinaObstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai, ChinaResearch Units of Embryo Original Diseases, Chinese Academy of Medical Sciences, Shanghai, ChinaKey Laboratory of Reproductive Genetics (Ministry of Education), Department of Reproductive Endocrinology, Women’s Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaPurposeTo study whether physical exercise can effectively ameliorate obesity-induced abnormalities in male fertility and provide a new perspective on the role of small noncoding RNAs in spermatogenesis in obese male mice.MethodsIn this study, four-week-old C57/Bl6 male mice were randomly allocated to receive a control diet, a high-fat diet or physical exercise intervention for 40 weeks. Purified round spermatids and spermatozoa were obtained after intervention. Sperm motility, concentration, the ability of the sperm to undergo capacitation and acrosome reaction were assessed. Small RNA sequencing was conducted on round spermatids and spermatozoa. The small noncoding RNAs expression pattern was systematically analyzed.ResultsThe spermatozoa concentration and percentage of motile spermatozoa, the capacitation and acrosome reaction, and the reproductive success rate, including mating success and pregnancy success, were decreased or delayed in the obesity group compared with controls. Physical exercise was able to restore the parameters to normal levels. Three microRNAs were consistently upregulated and 5 were downregulated in round spermatids and epididymal spermatozoa between the obesity and control groups.ConclusionsThis report provides evidence that the adverse effects of obesity could be offset after physical exercise. small noncoding RNAs, especially microRNAs in germ cells, may play an important role in the effects of obesity and physical exercise on spermatozoa.https://www.frontiersin.org/articles/10.3389/fendo.2022.1038449/fullmale infertilityobesityphysical exercisesncRNAmicroRNA |
| spellingShingle | Tingting Lin Shuyu Zhang Yuchuan Zhou Ligang Wu Xinmei Liu Hefeng Huang Hefeng Huang Hefeng Huang Hefeng Huang Small RNA perspective of physical exercise-related improvement of male reproductive dysfunction due to obesity Frontiers in Endocrinology male infertility obesity physical exercise sncRNA microRNA |
| title | Small RNA perspective of physical exercise-related improvement of male reproductive dysfunction due to obesity |
| title_full | Small RNA perspective of physical exercise-related improvement of male reproductive dysfunction due to obesity |
| title_fullStr | Small RNA perspective of physical exercise-related improvement of male reproductive dysfunction due to obesity |
| title_full_unstemmed | Small RNA perspective of physical exercise-related improvement of male reproductive dysfunction due to obesity |
| title_short | Small RNA perspective of physical exercise-related improvement of male reproductive dysfunction due to obesity |
| title_sort | small rna perspective of physical exercise related improvement of male reproductive dysfunction due to obesity |
| topic | male infertility obesity physical exercise sncRNA microRNA |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2022.1038449/full |
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