18S rDNA dataset profiling microeukaryotic populations within Chicago area nearshore waters

Despite their critical role in the aquatic food web and nutrient cycling, microeukaryotes within freshwater environments are under-studied. Herein we present the first high-throughput molecular survey of microeukaryotes within Lake Michigan. Every two weeks from May 13 to August 5, 2014, we collecte...

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Main Authors: Daniel Searle, Emily Sible, Alexandria Cooper, Catherine Putonti
Format: Article
Language:English
Published: Elsevier 2016-03-01
Series:Data in Brief
Online Access:http://www.sciencedirect.com/science/article/pii/S2352340915004084
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author Daniel Searle
Emily Sible
Alexandria Cooper
Catherine Putonti
author_facet Daniel Searle
Emily Sible
Alexandria Cooper
Catherine Putonti
author_sort Daniel Searle
collection DOAJ
description Despite their critical role in the aquatic food web and nutrient cycling, microeukaryotes within freshwater environments are under-studied. Herein we present the first high-throughput molecular survey of microeukaryotes within Lake Michigan. Every two weeks from May 13 to August 5, 2014, we collected surface water samples from the nearshore waters of four Chicago area beaches: Gillson Park, Montrose Beach, 57th Street Beach, and Calumet Beach. Four biological replicates were collected for each sampling date and location, resulting in 112 samples. Eighty-nine of these samples were surveyed through targeted sequencing of the V7 and V8 regions of the 18S rDNA gene. Both technical and biological replicates were sequenced and are included in this dataset. Raw sequence data is available via NCBI’s SRA database (BioProject PRJNA294919). Keywords: Microeukaryotic community, Metagenomics, Freshwater, Lake Michigan
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spelling doaj.art-7905c23e940a4fa8955225b947e6a1b62022-12-21T18:47:05ZengElsevierData in Brief2352-34092016-03-01652652918S rDNA dataset profiling microeukaryotic populations within Chicago area nearshore watersDaniel Searle0Emily Sible1Alexandria Cooper2Catherine Putonti3The Woodhall School, 58 Harrison Lane, Behlehem, CT 06751, USADepartment of Biology, Loyola University Chicago, 1032 W Sheridan Rd, Chicago, IL 60660, USADepartment of Biology, Loyola University Chicago, 1032 W Sheridan Rd, Chicago, IL 60660, USADepartment of Biology, Loyola University Chicago, 1032 W Sheridan Rd, Chicago, IL 60660, USA; Bioinformatics Program, Loyola University Chicago, 1032 W Sheridan Rd, Chicago, IL 60660, USA; Department of Computer Science, Loyola University Chicago, 820 N Michigan Ave, Chicago, IL 60611, USA; Corresponding author.Despite their critical role in the aquatic food web and nutrient cycling, microeukaryotes within freshwater environments are under-studied. Herein we present the first high-throughput molecular survey of microeukaryotes within Lake Michigan. Every two weeks from May 13 to August 5, 2014, we collected surface water samples from the nearshore waters of four Chicago area beaches: Gillson Park, Montrose Beach, 57th Street Beach, and Calumet Beach. Four biological replicates were collected for each sampling date and location, resulting in 112 samples. Eighty-nine of these samples were surveyed through targeted sequencing of the V7 and V8 regions of the 18S rDNA gene. Both technical and biological replicates were sequenced and are included in this dataset. Raw sequence data is available via NCBI’s SRA database (BioProject PRJNA294919). Keywords: Microeukaryotic community, Metagenomics, Freshwater, Lake Michiganhttp://www.sciencedirect.com/science/article/pii/S2352340915004084
spellingShingle Daniel Searle
Emily Sible
Alexandria Cooper
Catherine Putonti
18S rDNA dataset profiling microeukaryotic populations within Chicago area nearshore waters
Data in Brief
title 18S rDNA dataset profiling microeukaryotic populations within Chicago area nearshore waters
title_full 18S rDNA dataset profiling microeukaryotic populations within Chicago area nearshore waters
title_fullStr 18S rDNA dataset profiling microeukaryotic populations within Chicago area nearshore waters
title_full_unstemmed 18S rDNA dataset profiling microeukaryotic populations within Chicago area nearshore waters
title_short 18S rDNA dataset profiling microeukaryotic populations within Chicago area nearshore waters
title_sort 18s rdna dataset profiling microeukaryotic populations within chicago area nearshore waters
url http://www.sciencedirect.com/science/article/pii/S2352340915004084
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