Porcine Circovirus-Like Virus P1 Inhibits Wnt Signaling Pathway in Vivo and in Vitro
Porcine circovirus-like virus P1 is an important pathogen of the current pig industry, the infection mechanism is not entirely clear. Wnt signaling pathway plays an important role in the growth of young animals and infection of some viruses. This study was designed to demonstrate the effects of P1 i...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-03-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2018.00390/full |
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| author | Xuejiao Zhu Xuejiao Zhu Xuejiao Zhu Libin Wen Libin Wen Libin Wen Shaoyang Sheng Shaoyang Sheng Wei Wang Wei Wang Wei Wang Qi Xiao Qi Xiao Qi Xiao Meng Qu Meng Qu Yiyi Hu Yiyi Hu Yiyi Hu Chuanmin Liu Chuanmin Liu Chuanmin Liu Kongwang He Kongwang He Kongwang He |
| author_facet | Xuejiao Zhu Xuejiao Zhu Xuejiao Zhu Libin Wen Libin Wen Libin Wen Shaoyang Sheng Shaoyang Sheng Wei Wang Wei Wang Wei Wang Qi Xiao Qi Xiao Qi Xiao Meng Qu Meng Qu Yiyi Hu Yiyi Hu Yiyi Hu Chuanmin Liu Chuanmin Liu Chuanmin Liu Kongwang He Kongwang He Kongwang He |
| author_sort | Xuejiao Zhu |
| collection | DOAJ |
| description | Porcine circovirus-like virus P1 is an important pathogen of the current pig industry, the infection mechanism is not entirely clear. Wnt signaling pathway plays an important role in the growth of young animals and infection of some viruses. This study was designed to demonstrate the effects of P1 infection on the Wnt signaling pathway. In vivo experiments, we demonstrated the down-regulatory effects of P1 infection in piglets and mice on the downstream components expression levels of Wnt signaling pathway, and the effects of Wnt signaling pathway activation on the pathogenesis of P1. In vitro studies, we found P1 infection down-regulated protein level of β-catenin and mRNA level of mmp2, prevented the β-catenin from entering into nucleus, abolished the TCF/LEF promoter activity, proved that P1 could inhibit the activation of Wnt signaling pathway in vitro. Finally, we found that VP1 of P1 virus also had the inhibitory effects on Wnt signaling pathway in vitro, elucidated the mechanism of P1’s inhibitory effects on the Wnt signaling pathway and offered the possibility that the suppression of Wnt signaling pathway was involved in the post-weaning multisystemic wasting syndrome (PMWS), laying a foundation for elucidating the pathogenesis of P1. |
| first_indexed | 2024-04-12T23:53:40Z |
| format | Article |
| id | doaj.art-79087f08211c4ac093ec628736f71ddd |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T23:53:40Z |
| publishDate | 2018-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-79087f08211c4ac093ec628736f71ddd2022-12-22T03:11:36ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-03-01910.3389/fmicb.2018.00390336773Porcine Circovirus-Like Virus P1 Inhibits Wnt Signaling Pathway in Vivo and in VitroXuejiao Zhu0Xuejiao Zhu1Xuejiao Zhu2Libin Wen3Libin Wen4Libin Wen5Shaoyang Sheng6Shaoyang Sheng7Wei Wang8Wei Wang9Wei Wang10Qi Xiao11Qi Xiao12Qi Xiao13Meng Qu14Meng Qu15Yiyi Hu16Yiyi Hu17Yiyi Hu18Chuanmin Liu19Chuanmin Liu20Chuanmin Liu21Kongwang He22Kongwang He23Kongwang He24Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences – Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaJiangsu Key Laboratory for Food Quality and Safety – State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, ChinaInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences – Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaJiangsu Key Laboratory for Food Quality and Safety – State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, ChinaInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences – Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences – Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaJiangsu Key Laboratory for Food Quality and Safety – State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, ChinaInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences – Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaJiangsu Key Laboratory for Food Quality and Safety – State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, ChinaInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences – Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences – Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaJiangsu Key Laboratory for Food Quality and Safety – State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, ChinaInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences – Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaJiangsu Key Laboratory for Food Quality and Safety – State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, ChinaInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences – Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaJiangsu Key Laboratory for Food Quality and Safety – State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, ChinaPorcine circovirus-like virus P1 is an important pathogen of the current pig industry, the infection mechanism is not entirely clear. Wnt signaling pathway plays an important role in the growth of young animals and infection of some viruses. This study was designed to demonstrate the effects of P1 infection on the Wnt signaling pathway. In vivo experiments, we demonstrated the down-regulatory effects of P1 infection in piglets and mice on the downstream components expression levels of Wnt signaling pathway, and the effects of Wnt signaling pathway activation on the pathogenesis of P1. In vitro studies, we found P1 infection down-regulated protein level of β-catenin and mRNA level of mmp2, prevented the β-catenin from entering into nucleus, abolished the TCF/LEF promoter activity, proved that P1 could inhibit the activation of Wnt signaling pathway in vitro. Finally, we found that VP1 of P1 virus also had the inhibitory effects on Wnt signaling pathway in vitro, elucidated the mechanism of P1’s inhibitory effects on the Wnt signaling pathway and offered the possibility that the suppression of Wnt signaling pathway was involved in the post-weaning multisystemic wasting syndrome (PMWS), laying a foundation for elucidating the pathogenesis of P1.http://journal.frontiersin.org/article/10.3389/fmicb.2018.00390/fullporcine circovirus-like virus P1PMWSinhibit effectVP1Wnt signaling |
| spellingShingle | Xuejiao Zhu Xuejiao Zhu Xuejiao Zhu Libin Wen Libin Wen Libin Wen Shaoyang Sheng Shaoyang Sheng Wei Wang Wei Wang Wei Wang Qi Xiao Qi Xiao Qi Xiao Meng Qu Meng Qu Yiyi Hu Yiyi Hu Yiyi Hu Chuanmin Liu Chuanmin Liu Chuanmin Liu Kongwang He Kongwang He Kongwang He Porcine Circovirus-Like Virus P1 Inhibits Wnt Signaling Pathway in Vivo and in Vitro Frontiers in Microbiology porcine circovirus-like virus P1 PMWS inhibit effect VP1 Wnt signaling |
| title | Porcine Circovirus-Like Virus P1 Inhibits Wnt Signaling Pathway in Vivo and in Vitro |
| title_full | Porcine Circovirus-Like Virus P1 Inhibits Wnt Signaling Pathway in Vivo and in Vitro |
| title_fullStr | Porcine Circovirus-Like Virus P1 Inhibits Wnt Signaling Pathway in Vivo and in Vitro |
| title_full_unstemmed | Porcine Circovirus-Like Virus P1 Inhibits Wnt Signaling Pathway in Vivo and in Vitro |
| title_short | Porcine Circovirus-Like Virus P1 Inhibits Wnt Signaling Pathway in Vivo and in Vitro |
| title_sort | porcine circovirus like virus p1 inhibits wnt signaling pathway in vivo and in vitro |
| topic | porcine circovirus-like virus P1 PMWS inhibit effect VP1 Wnt signaling |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2018.00390/full |
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