Peganum harmala L.’s anti-growth effect on a breast cancer cell line
This research was done to evaluate the induction of apoptosis in MDA-MB-231 breast cancer cell line by Peganum harmala’s extract, in which a significant amount of ß-carbolines is included. The apoptosis incidence was assessed through Annexin-V-Flous kit. The expressions of genes through which intrin...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015-12-01
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| Series: | Biotechnology Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X15000491 |
| _version_ | 1819085960792506368 |
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| author | Somayeh Hashemi Sheikh Shabani Sahar Seyed Hasan Tehrani Zohreh Rabiei Sattar Tahmasebi Enferadi Gian Paolo Vannozzi |
| author_facet | Somayeh Hashemi Sheikh Shabani Sahar Seyed Hasan Tehrani Zohreh Rabiei Sattar Tahmasebi Enferadi Gian Paolo Vannozzi |
| author_sort | Somayeh Hashemi Sheikh Shabani |
| collection | DOAJ |
| description | This research was done to evaluate the induction of apoptosis in MDA-MB-231 breast cancer cell line by Peganum harmala’s extract, in which a significant amount of ß-carbolines is included. The apoptosis incidence was assessed through Annexin-V-Flous kit. The expressions of genes through which intrinsic apoptosis pathway are involved, Bax, Bcl-2, Bid, and Puma, over the genes the expressions of which are linked to extrinsic apoptosis pathway, TRAIL, Caspase8, p21, and p53, were examined by RT-PCR and Real-time PCR. The results demonstrate that the extract decreases the growth rate of the cancer cell line through inducing apoptosis mechanism. As long as the expression of anti-apoptosis Bcl-2 gen reduced dramatically, an over-expression in Bax and Puma genes was monitored indicating activation of intrinsic apoptosis pathway. A notable over-expression observed with TRAIL and Caspase8 genes as well as Bid gene. The latter is an intermediate for both intrinsic and extrinsic pathways of apoptosis. |
| first_indexed | 2024-12-21T21:12:40Z |
| format | Article |
| id | doaj.art-790b5f9630fb44cd8c66780b6b7aea4f |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-12-21T21:12:40Z |
| publishDate | 2015-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-790b5f9630fb44cd8c66780b6b7aea4f2022-12-21T18:50:06ZengElsevierBiotechnology Reports2215-017X2015-12-018C13814310.1016/j.btre.2015.08.007Peganum harmala L.’s anti-growth effect on a breast cancer cell lineSomayeh Hashemi Sheikh Shabani0Sahar Seyed Hasan Tehrani1Zohreh Rabiei2Sattar Tahmasebi Enferadi3Gian Paolo Vannozzi4National Institute of Genetic Engineering and Biotechnology, Tehran, IranNational Institute of Genetic Engineering and Biotechnology, Tehran, IranNational Institute of Genetic Engineering and Biotechnology, Tehran, IranNational Institute of Genetic Engineering and Biotechnology, Tehran, IranDipartimenti di Sienze Agraria, Universita degli Studi di Udine, Udine, ItalyThis research was done to evaluate the induction of apoptosis in MDA-MB-231 breast cancer cell line by Peganum harmala’s extract, in which a significant amount of ß-carbolines is included. The apoptosis incidence was assessed through Annexin-V-Flous kit. The expressions of genes through which intrinsic apoptosis pathway are involved, Bax, Bcl-2, Bid, and Puma, over the genes the expressions of which are linked to extrinsic apoptosis pathway, TRAIL, Caspase8, p21, and p53, were examined by RT-PCR and Real-time PCR. The results demonstrate that the extract decreases the growth rate of the cancer cell line through inducing apoptosis mechanism. As long as the expression of anti-apoptosis Bcl-2 gen reduced dramatically, an over-expression in Bax and Puma genes was monitored indicating activation of intrinsic apoptosis pathway. A notable over-expression observed with TRAIL and Caspase8 genes as well as Bid gene. The latter is an intermediate for both intrinsic and extrinsic pathways of apoptosis.http://www.sciencedirect.com/science/article/pii/S2215017X15000491Extrinsic/intrinsic apoptosisHarmalineHarmine |
| spellingShingle | Somayeh Hashemi Sheikh Shabani Sahar Seyed Hasan Tehrani Zohreh Rabiei Sattar Tahmasebi Enferadi Gian Paolo Vannozzi Peganum harmala L.’s anti-growth effect on a breast cancer cell line Biotechnology Reports Extrinsic/intrinsic apoptosis Harmaline Harmine |
| title | Peganum harmala L.’s anti-growth effect on a breast cancer cell line |
| title_full | Peganum harmala L.’s anti-growth effect on a breast cancer cell line |
| title_fullStr | Peganum harmala L.’s anti-growth effect on a breast cancer cell line |
| title_full_unstemmed | Peganum harmala L.’s anti-growth effect on a breast cancer cell line |
| title_short | Peganum harmala L.’s anti-growth effect on a breast cancer cell line |
| title_sort | peganum harmala l s anti growth effect on a breast cancer cell line |
| topic | Extrinsic/intrinsic apoptosis Harmaline Harmine |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X15000491 |
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