Phytochemical Composition and In Vitro Biological Activity of <i>Iris</i> spp. (Iridaceae): A New Source of Bioactive Constituents for the Inhibition of Oral Bacterial Biofilms
The inhibition and eradication of oral biofilms is increasingly focused on the use of plant extracts as mouthwashes and toothpastes adjuvants. Here, we report on the chemical composition and the antibiofilm activity of 15 methanolic extracts of <i>Iris</i> species against both mono-(<...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/9/7/403 |
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| author | Lan Hoang František Beneš Marie Fenclová Olga Kronusová Viviana Švarcová Kateřina Řehořová Eva Baldassarre Švecová Miroslav Vosátka Jana Hajšlová Petr Kaštánek Jitka Viktorová Tomáš Ruml |
| author_facet | Lan Hoang František Beneš Marie Fenclová Olga Kronusová Viviana Švarcová Kateřina Řehořová Eva Baldassarre Švecová Miroslav Vosátka Jana Hajšlová Petr Kaštánek Jitka Viktorová Tomáš Ruml |
| author_sort | Lan Hoang |
| collection | DOAJ |
| description | The inhibition and eradication of oral biofilms is increasingly focused on the use of plant extracts as mouthwashes and toothpastes adjuvants. Here, we report on the chemical composition and the antibiofilm activity of 15 methanolic extracts of <i>Iris</i> species against both mono-(<i>Pseudomonas aeruginosa</i>, <i>Staphylococcus aureus</i>) and multi-species oral biofilms (<i>Streptococcus gordonii, Veillonella parvula, Fusobacterium nucleatum</i> subsp. <i>nucleatum</i>, and <i>Actinomyces naeslundii</i>). The phytochemical profiles of <i>Iris pallida</i> s.l., <i>Iris versicolor</i> L., <i>Iris lactea</i> Pall., <i>Iris carthaliniae</i> Fomin, and <i>Iris germanica</i> were determined by ultra-high performance liquid chromatography-high-resolution tandem mass spectroscopy (UHPLC-HRMS/MS) analysis, and a total of 180 compounds were identified among <i>Iris</i> species with (iso)flavonoid dominancy. <i>I. pallida, I. versicolor,</i> and <i>I. germanica</i> inhibited both the quorum sensing and adhesion during biofilm formation in a concentration-dependent manner. However, the extracts were less active against maturated biofilms. Of the five tested species, <i>Iris pallida</i> s.l. was the most effective at both inhibiting biofilm formation and disrupting existing biofilms, and the leaf extract exhibited the strongest inhibitory effect compared to the root and rhizome extracts. The cytotoxicity of the extracts was excluded in human fibroblasts. The inhibition of bacterial adhesion significantly correlated with myristic acid content, and quorum sensing inhibition correlated with the 7-β-hydroxystigmast-4-en-3-one content. These findings could be useful for establishing an effective tool for the control of oral biofilms and thus dental diseases. |
| first_indexed | 2024-03-10T18:32:41Z |
| format | Article |
| id | doaj.art-790b9b6dd067401c9008be4ab66794ea |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T18:32:41Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-790b9b6dd067401c9008be4ab66794ea2023-11-20T06:30:28ZengMDPI AGAntibiotics2079-63822020-07-019740310.3390/antibiotics9070403Phytochemical Composition and In Vitro Biological Activity of <i>Iris</i> spp. (Iridaceae): A New Source of Bioactive Constituents for the Inhibition of Oral Bacterial BiofilmsLan Hoang0František Beneš1Marie Fenclová2Olga Kronusová3Viviana Švarcová4Kateřina Řehořová5Eva Baldassarre Švecová6Miroslav Vosátka7Jana Hajšlová8Petr Kaštánek9Jitka Viktorová10Tomáš Ruml11Department of Biochemistry and Microbiology UCT Prague, Faculty of Food and Biochemical Technology, Technická 3, 166 28 Prague, Czech RepublicDepartment of Food Analysis and Nutrition UCT Prague, Faculty of Food and Biochemical Technology, Technická 3, 166 28 Prague, Czech RepublicDepartment of Food Analysis and Nutrition UCT Prague, Faculty of Food and Biochemical Technology, Technická 3, 166 28 Prague, Czech RepublicDepartment of Biochemistry and Microbiology UCT Prague, Faculty of Food and Biochemical Technology, Technická 3, 166 28 Prague, Czech RepublicDepartment of Biochemistry and Microbiology UCT Prague, Faculty of Food and Biochemical Technology, Technická 3, 166 28 Prague, Czech RepublicDepartment of Biochemistry and Microbiology UCT Prague, Faculty of Food and Biochemical Technology, Technická 3, 166 28 Prague, Czech RepublicInstitute of Botany of the Czech Academy of Sciences, Zámek 1, 252 43 Průhonice, Czech RepublicInstitute of Botany of the Czech Academy of Sciences, Zámek 1, 252 43 Průhonice, Czech RepublicDepartment of Food Analysis and Nutrition UCT Prague, Faculty of Food and Biochemical Technology, Technická 3, 166 28 Prague, Czech RepublicEcoFuel Laboratories Ltd., Ocelářská 9, 190 00 Praha, Czech RepublicDepartment of Biochemistry and Microbiology UCT Prague, Faculty of Food and Biochemical Technology, Technická 3, 166 28 Prague, Czech RepublicDepartment of Biochemistry and Microbiology UCT Prague, Faculty of Food and Biochemical Technology, Technická 3, 166 28 Prague, Czech RepublicThe inhibition and eradication of oral biofilms is increasingly focused on the use of plant extracts as mouthwashes and toothpastes adjuvants. Here, we report on the chemical composition and the antibiofilm activity of 15 methanolic extracts of <i>Iris</i> species against both mono-(<i>Pseudomonas aeruginosa</i>, <i>Staphylococcus aureus</i>) and multi-species oral biofilms (<i>Streptococcus gordonii, Veillonella parvula, Fusobacterium nucleatum</i> subsp. <i>nucleatum</i>, and <i>Actinomyces naeslundii</i>). The phytochemical profiles of <i>Iris pallida</i> s.l., <i>Iris versicolor</i> L., <i>Iris lactea</i> Pall., <i>Iris carthaliniae</i> Fomin, and <i>Iris germanica</i> were determined by ultra-high performance liquid chromatography-high-resolution tandem mass spectroscopy (UHPLC-HRMS/MS) analysis, and a total of 180 compounds were identified among <i>Iris</i> species with (iso)flavonoid dominancy. <i>I. pallida, I. versicolor,</i> and <i>I. germanica</i> inhibited both the quorum sensing and adhesion during biofilm formation in a concentration-dependent manner. However, the extracts were less active against maturated biofilms. Of the five tested species, <i>Iris pallida</i> s.l. was the most effective at both inhibiting biofilm formation and disrupting existing biofilms, and the leaf extract exhibited the strongest inhibitory effect compared to the root and rhizome extracts. The cytotoxicity of the extracts was excluded in human fibroblasts. The inhibition of bacterial adhesion significantly correlated with myristic acid content, and quorum sensing inhibition correlated with the 7-β-hydroxystigmast-4-en-3-one content. These findings could be useful for establishing an effective tool for the control of oral biofilms and thus dental diseases.https://www.mdpi.com/2079-6382/9/7/403biofilmdental plaquequorum sensingmicrobial resistance |
| spellingShingle | Lan Hoang František Beneš Marie Fenclová Olga Kronusová Viviana Švarcová Kateřina Řehořová Eva Baldassarre Švecová Miroslav Vosátka Jana Hajšlová Petr Kaštánek Jitka Viktorová Tomáš Ruml Phytochemical Composition and In Vitro Biological Activity of <i>Iris</i> spp. (Iridaceae): A New Source of Bioactive Constituents for the Inhibition of Oral Bacterial Biofilms Antibiotics biofilm dental plaque quorum sensing microbial resistance |
| title | Phytochemical Composition and In Vitro Biological Activity of <i>Iris</i> spp. (Iridaceae): A New Source of Bioactive Constituents for the Inhibition of Oral Bacterial Biofilms |
| title_full | Phytochemical Composition and In Vitro Biological Activity of <i>Iris</i> spp. (Iridaceae): A New Source of Bioactive Constituents for the Inhibition of Oral Bacterial Biofilms |
| title_fullStr | Phytochemical Composition and In Vitro Biological Activity of <i>Iris</i> spp. (Iridaceae): A New Source of Bioactive Constituents for the Inhibition of Oral Bacterial Biofilms |
| title_full_unstemmed | Phytochemical Composition and In Vitro Biological Activity of <i>Iris</i> spp. (Iridaceae): A New Source of Bioactive Constituents for the Inhibition of Oral Bacterial Biofilms |
| title_short | Phytochemical Composition and In Vitro Biological Activity of <i>Iris</i> spp. (Iridaceae): A New Source of Bioactive Constituents for the Inhibition of Oral Bacterial Biofilms |
| title_sort | phytochemical composition and in vitro biological activity of i iris i spp iridaceae a new source of bioactive constituents for the inhibition of oral bacterial biofilms |
| topic | biofilm dental plaque quorum sensing microbial resistance |
| url | https://www.mdpi.com/2079-6382/9/7/403 |
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