Effects of dietary yeast cell wall supplementation on growth performance, intestinal Campylobacter jejuni colonization, innate immune response, villus height, crypt depth, and slaughter characteristics of broiler chickens inoculated with Campylobacter jejuni at d 21
ABSTRACT: A study was conducted to assess the effects of a dietary yeast cell wall (YCW) with and without a Campylobacter jejuni (CJ) challenge. A total of 2,240-day-old Ross 708 males were randomly assigned within 8 treatments with a 4 × 2 factorial design, with 4 diets (negative control, positive...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-05-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579123001335 |
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| author | Luis R. Munoz Matthew A. Bailey James T. Krehling Dianna V. Bourassa Ruediger Hauck Wilmer J. Pacheco Bernardo Chaves-Cordoba Kaicie S. Chasteen Aidan A. Talorico Cesar Escobar Andrea Pietruska Ken S. Macklin |
| author_facet | Luis R. Munoz Matthew A. Bailey James T. Krehling Dianna V. Bourassa Ruediger Hauck Wilmer J. Pacheco Bernardo Chaves-Cordoba Kaicie S. Chasteen Aidan A. Talorico Cesar Escobar Andrea Pietruska Ken S. Macklin |
| author_sort | Luis R. Munoz |
| collection | DOAJ |
| description | ABSTRACT: A study was conducted to assess the effects of a dietary yeast cell wall (YCW) with and without a Campylobacter jejuni (CJ) challenge. A total of 2,240-day-old Ross 708 males were randomly assigned within 8 treatments with a 4 × 2 factorial design, with 4 diets (negative control, positive control, YCW constant dose (400 g/ton), and YCW step-down dose (800/400/200 g/ton in the starter/grower/finisher diets, respectively) and with and without d 21 CJ oral gavage challenge at 5.2 × 107 CFU/mL. At d 0, 14, 28, and 41 body weights and feed consumption were measured to determine performance. At d 14, 28, and 42, 8 jejunal and ileal histology samples per treatment were collected for villi morphology measurements. At d 22 and 28 (1- and 7-days postinoculation), 24 ileal tissue samples per treatment were collected for relative gene expression analysis. At d 42, 24 cecal content samples per treatment were collected for CJ enumeration. Finally, on d 44, 96 birds per treatment were processed to determine carcass yield and 16 carcass rinses per treatment were collected to determine CJ prevalence after processing. Diet or inoculation did not impact broiler performance (P > 0.05). Limited differences were observed in intestinal morphology, and villus height and crypt depth were different only in the ileum at d 42 (P = 0.0280 and P = 0.0162, respectively). At d 1 postinoculation, differences between treatments inoculated with CJ and PBS were observed in the expression of avian beta defensin 10 (AvBD10), interleukin 1ß (IL-1ß), and interleukin 10 (IL-10) (P < 0.05). At d 7 postinoculation, expression of AvBD10, IL-1ß, and IL-10 was similar among all treatments (P > 0.05). At d 42, all birds, regardless the inoculation, had similar levels of CJ recovered from cecal contents (P > 0.05). After processing, carcass yield and CJ prevalence postchilling was similar in all treatments (P > 0.05). Overall, under the conditions of this study, the addition of YCW during a CJ challenge did not have an impact in growth performance, innate immune response, cecal colonization, carcass yield, or CJ prevalence after processing. |
| first_indexed | 2024-04-09T15:21:14Z |
| format | Article |
| id | doaj.art-790bd0123f6345db94f672dfc92c8c9c |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-09T15:21:14Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-790bd0123f6345db94f672dfc92c8c9c2023-04-29T14:45:41ZengElsevierPoultry Science0032-57912023-05-011025102609Effects of dietary yeast cell wall supplementation on growth performance, intestinal Campylobacter jejuni colonization, innate immune response, villus height, crypt depth, and slaughter characteristics of broiler chickens inoculated with Campylobacter jejuni at d 21Luis R. Munoz0Matthew A. Bailey1James T. Krehling2Dianna V. Bourassa3Ruediger Hauck4Wilmer J. Pacheco5Bernardo Chaves-Cordoba6Kaicie S. Chasteen7Aidan A. Talorico8Cesar Escobar9Andrea Pietruska10Ken S. Macklin11Department of Poultry Science, Auburn University, Auburn, AL 36849, USADepartment of Poultry Science, Auburn University, Auburn, AL 36849, USADepartment of Poultry Science, Auburn University, Auburn, AL 36849, USADepartment of Poultry Science, Auburn University, Auburn, AL 36849, USADepartment of Pathobiology, Auburn University, Auburn, AL 36849, USADepartment of Poultry Science, Auburn University, Auburn, AL 36849, USACollege of Agriculture, Auburn University, Auburn, AL 36832, USADepartment of Poultry Science, Auburn University, Auburn, AL 36849, USADepartment of Poultry Science, Auburn University, Auburn, AL 36849, USADepartment of Poultry Science, Auburn University, Auburn, AL 36849, USADepartment of Pathobiology, Auburn University, Auburn, AL 36849, USADepartment of Poultry Science, Auburn University, Auburn, AL 36849, USA; Corresponding author:ABSTRACT: A study was conducted to assess the effects of a dietary yeast cell wall (YCW) with and without a Campylobacter jejuni (CJ) challenge. A total of 2,240-day-old Ross 708 males were randomly assigned within 8 treatments with a 4 × 2 factorial design, with 4 diets (negative control, positive control, YCW constant dose (400 g/ton), and YCW step-down dose (800/400/200 g/ton in the starter/grower/finisher diets, respectively) and with and without d 21 CJ oral gavage challenge at 5.2 × 107 CFU/mL. At d 0, 14, 28, and 41 body weights and feed consumption were measured to determine performance. At d 14, 28, and 42, 8 jejunal and ileal histology samples per treatment were collected for villi morphology measurements. At d 22 and 28 (1- and 7-days postinoculation), 24 ileal tissue samples per treatment were collected for relative gene expression analysis. At d 42, 24 cecal content samples per treatment were collected for CJ enumeration. Finally, on d 44, 96 birds per treatment were processed to determine carcass yield and 16 carcass rinses per treatment were collected to determine CJ prevalence after processing. Diet or inoculation did not impact broiler performance (P > 0.05). Limited differences were observed in intestinal morphology, and villus height and crypt depth were different only in the ileum at d 42 (P = 0.0280 and P = 0.0162, respectively). At d 1 postinoculation, differences between treatments inoculated with CJ and PBS were observed in the expression of avian beta defensin 10 (AvBD10), interleukin 1ß (IL-1ß), and interleukin 10 (IL-10) (P < 0.05). At d 7 postinoculation, expression of AvBD10, IL-1ß, and IL-10 was similar among all treatments (P > 0.05). At d 42, all birds, regardless the inoculation, had similar levels of CJ recovered from cecal contents (P > 0.05). After processing, carcass yield and CJ prevalence postchilling was similar in all treatments (P > 0.05). Overall, under the conditions of this study, the addition of YCW during a CJ challenge did not have an impact in growth performance, innate immune response, cecal colonization, carcass yield, or CJ prevalence after processing.http://www.sciencedirect.com/science/article/pii/S0032579123001335Campylobacter jejunibroilercolonizationperformanceyeast cell wall |
| spellingShingle | Luis R. Munoz Matthew A. Bailey James T. Krehling Dianna V. Bourassa Ruediger Hauck Wilmer J. Pacheco Bernardo Chaves-Cordoba Kaicie S. Chasteen Aidan A. Talorico Cesar Escobar Andrea Pietruska Ken S. Macklin Effects of dietary yeast cell wall supplementation on growth performance, intestinal Campylobacter jejuni colonization, innate immune response, villus height, crypt depth, and slaughter characteristics of broiler chickens inoculated with Campylobacter jejuni at d 21 Poultry Science Campylobacter jejuni broiler colonization performance yeast cell wall |
| title | Effects of dietary yeast cell wall supplementation on growth performance, intestinal Campylobacter jejuni colonization, innate immune response, villus height, crypt depth, and slaughter characteristics of broiler chickens inoculated with Campylobacter jejuni at d 21 |
| title_full | Effects of dietary yeast cell wall supplementation on growth performance, intestinal Campylobacter jejuni colonization, innate immune response, villus height, crypt depth, and slaughter characteristics of broiler chickens inoculated with Campylobacter jejuni at d 21 |
| title_fullStr | Effects of dietary yeast cell wall supplementation on growth performance, intestinal Campylobacter jejuni colonization, innate immune response, villus height, crypt depth, and slaughter characteristics of broiler chickens inoculated with Campylobacter jejuni at d 21 |
| title_full_unstemmed | Effects of dietary yeast cell wall supplementation on growth performance, intestinal Campylobacter jejuni colonization, innate immune response, villus height, crypt depth, and slaughter characteristics of broiler chickens inoculated with Campylobacter jejuni at d 21 |
| title_short | Effects of dietary yeast cell wall supplementation on growth performance, intestinal Campylobacter jejuni colonization, innate immune response, villus height, crypt depth, and slaughter characteristics of broiler chickens inoculated with Campylobacter jejuni at d 21 |
| title_sort | effects of dietary yeast cell wall supplementation on growth performance intestinal campylobacter jejuni colonization innate immune response villus height crypt depth and slaughter characteristics of broiler chickens inoculated with campylobacter jejuni at d 21 |
| topic | Campylobacter jejuni broiler colonization performance yeast cell wall |
| url | http://www.sciencedirect.com/science/article/pii/S0032579123001335 |
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