Insecticidal toxicity of essential oil of Nepalese Acorus calamus (Acorales:Acoraceae) against Sitophilus zeamais (Coleoptera:Curculionidae)
Maize weevil (Sitophilus zeamais) (Coleoptera:Curculionidae) is an economic stored grain pest that causes significant damage to various stored products, including maize (Zea mays). In this study, we extracted essential oil from the rhizome of sweet flag (Acorus calamus) (Acorales:Acoraceae) by hydro...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023093386 |
| _version_ | 1827615873037238272 |
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| author | Sunil Aryal Ashmita Poudel Kapil Kafle Lok Nath Aryal |
| author_facet | Sunil Aryal Ashmita Poudel Kapil Kafle Lok Nath Aryal |
| author_sort | Sunil Aryal |
| collection | DOAJ |
| description | Maize weevil (Sitophilus zeamais) (Coleoptera:Curculionidae) is an economic stored grain pest that causes significant damage to various stored products, including maize (Zea mays). In this study, we extracted essential oil from the rhizome of sweet flag (Acorus calamus) (Acorales:Acoraceae) by hydro-distillation and tested insecticidal property of the oil at 7 concentrations (10, 5, 2.5, 1.25, 0.625, 0.3125, 0.15625 and control) against maize weevil (Sitophilus zeamais) at the National Entomology Research Center, Nepal Agricultural Research Council in the year 2020/2021. Three different experiments were conducted: scintillating vial bioassay, repellency test, and exposing weevils to oil treated maize grains. Scintillating vial bioassay showed that higher the concentration of essential oil, lower the time required to cause 50 % maize weevil mortality. Median lethal concentration (LC50) at 3 and 24 h was calculated as 2.29 and 0.16 % of oil concentration in scintillating vial bioassay. When oil is treated to maize grain, LC50 for 10 and 16 days was calculated as 2.77 and 0.23 % of oil concentrations. In the same way, at 10 % concentration maize weevil showed highest repellent activity (98.75 %) as compared to 5, 2.5 and 1.25 % concentrations after 24 h of treatment. Weight loss and grain damage were significantly less in the oil treatments than the control. However, from the perspective of health benefits, Acorus calamus treated maize is still questionable for feed and food purpose. As β asarone has carcinogenic effects at certain level, it needs further residue tests of treated maize to know allowable maximum residue limit (MRL) before consumption as food or feed. |
| first_indexed | 2024-03-09T09:16:52Z |
| format | Article |
| id | doaj.art-790c6dd3ea624b2c8189906339c6aa5d |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-09T09:16:52Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-790c6dd3ea624b2c8189906339c6aa5d2023-12-02T07:05:16ZengElsevierHeliyon2405-84402023-11-01911e22130Insecticidal toxicity of essential oil of Nepalese Acorus calamus (Acorales:Acoraceae) against Sitophilus zeamais (Coleoptera:Curculionidae)Sunil Aryal0Ashmita Poudel1Kapil Kafle2Lok Nath Aryal3Horticulture Research Station, Pokhara, Kaski, Nepal Agricultural Research Council, Nepal; Corresponding author.Tribhuvan University, Institute of Agriculture and Animal Sciences, Kirtipur, Kathmandu, NepalTribhuvan University, Institute of Agriculture and Animal Sciences, Kirtipur, Kathmandu, NepalHorticulture Research Station, Pokhara, Kaski, Nepal Agricultural Research Council, NepalMaize weevil (Sitophilus zeamais) (Coleoptera:Curculionidae) is an economic stored grain pest that causes significant damage to various stored products, including maize (Zea mays). In this study, we extracted essential oil from the rhizome of sweet flag (Acorus calamus) (Acorales:Acoraceae) by hydro-distillation and tested insecticidal property of the oil at 7 concentrations (10, 5, 2.5, 1.25, 0.625, 0.3125, 0.15625 and control) against maize weevil (Sitophilus zeamais) at the National Entomology Research Center, Nepal Agricultural Research Council in the year 2020/2021. Three different experiments were conducted: scintillating vial bioassay, repellency test, and exposing weevils to oil treated maize grains. Scintillating vial bioassay showed that higher the concentration of essential oil, lower the time required to cause 50 % maize weevil mortality. Median lethal concentration (LC50) at 3 and 24 h was calculated as 2.29 and 0.16 % of oil concentration in scintillating vial bioassay. When oil is treated to maize grain, LC50 for 10 and 16 days was calculated as 2.77 and 0.23 % of oil concentrations. In the same way, at 10 % concentration maize weevil showed highest repellent activity (98.75 %) as compared to 5, 2.5 and 1.25 % concentrations after 24 h of treatment. Weight loss and grain damage were significantly less in the oil treatments than the control. However, from the perspective of health benefits, Acorus calamus treated maize is still questionable for feed and food purpose. As β asarone has carcinogenic effects at certain level, it needs further residue tests of treated maize to know allowable maximum residue limit (MRL) before consumption as food or feed.http://www.sciencedirect.com/science/article/pii/S2405844023093386Sweet flagMaize weevilOil extractionConcentrationsManagement |
| spellingShingle | Sunil Aryal Ashmita Poudel Kapil Kafle Lok Nath Aryal Insecticidal toxicity of essential oil of Nepalese Acorus calamus (Acorales:Acoraceae) against Sitophilus zeamais (Coleoptera:Curculionidae) Heliyon Sweet flag Maize weevil Oil extraction Concentrations Management |
| title | Insecticidal toxicity of essential oil of Nepalese Acorus calamus (Acorales:Acoraceae) against Sitophilus zeamais (Coleoptera:Curculionidae) |
| title_full | Insecticidal toxicity of essential oil of Nepalese Acorus calamus (Acorales:Acoraceae) against Sitophilus zeamais (Coleoptera:Curculionidae) |
| title_fullStr | Insecticidal toxicity of essential oil of Nepalese Acorus calamus (Acorales:Acoraceae) against Sitophilus zeamais (Coleoptera:Curculionidae) |
| title_full_unstemmed | Insecticidal toxicity of essential oil of Nepalese Acorus calamus (Acorales:Acoraceae) against Sitophilus zeamais (Coleoptera:Curculionidae) |
| title_short | Insecticidal toxicity of essential oil of Nepalese Acorus calamus (Acorales:Acoraceae) against Sitophilus zeamais (Coleoptera:Curculionidae) |
| title_sort | insecticidal toxicity of essential oil of nepalese acorus calamus acorales acoraceae against sitophilus zeamais coleoptera curculionidae |
| topic | Sweet flag Maize weevil Oil extraction Concentrations Management |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023093386 |
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