Egg Shell Membrane as an Alternative Vascular Patch for Arterial Angioplasty
Introduction: The egg shell membrane (ESM) is always considered as waste, but recent studies have shown that it has the potential to yield rapid re-endothelialization in vitro. We hypothesized that ESM and heparin-conjugated ESM (HESM) can be used as arterial patch in a rat aortic angioplasty model....
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.843590/full |
| _version_ | 1819003056458563584 |
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| author | Peng Sun Peng Sun Shujie Yan Shujie Yan Liwei Zhang Cong Zhang Haoliang Wu Shunbo Wei Boao Xie Xiaofeng Wang Xiaofeng Wang Hualong Bai Hualong Bai |
| author_facet | Peng Sun Peng Sun Shujie Yan Shujie Yan Liwei Zhang Cong Zhang Haoliang Wu Shunbo Wei Boao Xie Xiaofeng Wang Xiaofeng Wang Hualong Bai Hualong Bai |
| author_sort | Peng Sun |
| collection | DOAJ |
| description | Introduction: The egg shell membrane (ESM) is always considered as waste, but recent studies have shown that it has the potential to yield rapid re-endothelialization in vitro. We hypothesized that ESM and heparin-conjugated ESM (HESM) can be used as arterial patch in a rat aortic angioplasty model.Method: Sprague-Dawley rat (200 g) abdominal aortic patch angioplasty model was used. Decellularized rat thoracic aorta (TA) patch was used as the control; ESM patch was made of raw chicken egg; heparin-coated ESM (HESM) patch was made by using dopamine; anticoagulation properties were verified using platelet adhesion tests; the TA, ESM, and HESM patches were implanted to the rat aorta and harvested at day 14; and the samples were examined by immunohistochemistry and immunofluorescence.Result: The ESM patch showed a similar healing process to the TA patch; the cells could migrate and infiltrate into both patches; there was a neointima with von Willebrand factor-positive endothelial cells; the endothelial cells acquired arterial identity with Ephrin-B2- and dll-4-positive cells; there were proliferating cell nuclear antigen (PCNA)-positive cells, and PCNA and alpha smooth muscle actin dual-positive cells in the neointima in both groups. Heparin was conjugated to the patch successfully and showed a strong anticoagulation property in vitro. HESM could decrease mural thrombus formation after rat aortic patch angioplasty.Conclusion: The ESM is a natural scaffold that can be used as a vascular patch; it showed a similar healing process to decellularized TA patch; HESM showed anticoagulation property both in vitro and in vivo; and the ESM may be a promising vascular graft in the clinic. |
| first_indexed | 2024-12-20T23:14:56Z |
| format | Article |
| id | doaj.art-790c8ce707414ac49461b6922b7b5d78 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-20T23:14:56Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-790c8ce707414ac49461b6922b7b5d782022-12-21T19:23:39ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-03-011010.3389/fbioe.2022.843590843590Egg Shell Membrane as an Alternative Vascular Patch for Arterial AngioplastyPeng Sun0Peng Sun1Shujie Yan2Shujie Yan3Liwei Zhang4Cong Zhang5Haoliang Wu6Shunbo Wei7Boao Xie8Xiaofeng Wang9Xiaofeng Wang10Hualong Bai11Hualong Bai12Department of Vascular and Endovascular Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaKey Vascular Physiology and Applied Research Laboratory of Zhengzhou City, Zhengzhou, ChinaNational Center for International Research of Micro-Nano Molding Technology, Key Laboratory of Henan Province for Micro Molding Technology, Zhengzhou, ChinaSchool of Mechanics Science and Safety Engineering, Zhengzhou University, Zhengzhou, ChinaDepartment of Vascular and Endovascular Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Vascular and Endovascular Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Vascular and Endovascular Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Vascular and Endovascular Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Vascular and Endovascular Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaNational Center for International Research of Micro-Nano Molding Technology, Key Laboratory of Henan Province for Micro Molding Technology, Zhengzhou, ChinaSchool of Mechanics Science and Safety Engineering, Zhengzhou University, Zhengzhou, ChinaDepartment of Vascular and Endovascular Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaKey Vascular Physiology and Applied Research Laboratory of Zhengzhou City, Zhengzhou, ChinaIntroduction: The egg shell membrane (ESM) is always considered as waste, but recent studies have shown that it has the potential to yield rapid re-endothelialization in vitro. We hypothesized that ESM and heparin-conjugated ESM (HESM) can be used as arterial patch in a rat aortic angioplasty model.Method: Sprague-Dawley rat (200 g) abdominal aortic patch angioplasty model was used. Decellularized rat thoracic aorta (TA) patch was used as the control; ESM patch was made of raw chicken egg; heparin-coated ESM (HESM) patch was made by using dopamine; anticoagulation properties were verified using platelet adhesion tests; the TA, ESM, and HESM patches were implanted to the rat aorta and harvested at day 14; and the samples were examined by immunohistochemistry and immunofluorescence.Result: The ESM patch showed a similar healing process to the TA patch; the cells could migrate and infiltrate into both patches; there was a neointima with von Willebrand factor-positive endothelial cells; the endothelial cells acquired arterial identity with Ephrin-B2- and dll-4-positive cells; there were proliferating cell nuclear antigen (PCNA)-positive cells, and PCNA and alpha smooth muscle actin dual-positive cells in the neointima in both groups. Heparin was conjugated to the patch successfully and showed a strong anticoagulation property in vitro. HESM could decrease mural thrombus formation after rat aortic patch angioplasty.Conclusion: The ESM is a natural scaffold that can be used as a vascular patch; it showed a similar healing process to decellularized TA patch; HESM showed anticoagulation property both in vitro and in vivo; and the ESM may be a promising vascular graft in the clinic.https://www.frontiersin.org/articles/10.3389/fbioe.2022.843590/fullegg shell membranepatch angioplastydecellularizationheparinneointimal hyperplasia |
| spellingShingle | Peng Sun Peng Sun Shujie Yan Shujie Yan Liwei Zhang Cong Zhang Haoliang Wu Shunbo Wei Boao Xie Xiaofeng Wang Xiaofeng Wang Hualong Bai Hualong Bai Egg Shell Membrane as an Alternative Vascular Patch for Arterial Angioplasty Frontiers in Bioengineering and Biotechnology egg shell membrane patch angioplasty decellularization heparin neointimal hyperplasia |
| title | Egg Shell Membrane as an Alternative Vascular Patch for Arterial Angioplasty |
| title_full | Egg Shell Membrane as an Alternative Vascular Patch for Arterial Angioplasty |
| title_fullStr | Egg Shell Membrane as an Alternative Vascular Patch for Arterial Angioplasty |
| title_full_unstemmed | Egg Shell Membrane as an Alternative Vascular Patch for Arterial Angioplasty |
| title_short | Egg Shell Membrane as an Alternative Vascular Patch for Arterial Angioplasty |
| title_sort | egg shell membrane as an alternative vascular patch for arterial angioplasty |
| topic | egg shell membrane patch angioplasty decellularization heparin neointimal hyperplasia |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.843590/full |
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