Porcine Sapovirus in Northern Vietnam: Genetic Detection and Characterization Reveals Co-Circulation of Multiple Genotypes
Porcine sapovirus (PoSaV) has been reported in many countries over the world, which may cause gastroenteritis symptoms in pigs with all ages. There has been no report on PoSaV infection in Vietnam up to now. In this study, a total of 102 samples were collected from piglets, fattening pigs, and sows...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/10/7/430 |
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| author | Hieu Van Dong Thai Ha Truong Giang Thi Huong Tran Witsanu Rapichai Amonpun Rattanasrisomporn Kiattawee Choowongkomon Jatuporn Rattanasrisomporn |
| author_facet | Hieu Van Dong Thai Ha Truong Giang Thi Huong Tran Witsanu Rapichai Amonpun Rattanasrisomporn Kiattawee Choowongkomon Jatuporn Rattanasrisomporn |
| author_sort | Hieu Van Dong |
| collection | DOAJ |
| description | Porcine sapovirus (PoSaV) has been reported in many countries over the world, which may cause gastroenteritis symptoms in pigs with all ages. There has been no report on PoSaV infection in Vietnam up to now. In this study, a total of 102 samples were collected from piglets, fattening pigs, and sows with diarrhea in several cities and provinces in northern Vietnam. The PoSaV genome was examined using polymerase chain reaction (PCR). Sequencing of the partial RNA-dependent RNA polymerase (RdRp) gene sequences (324 bp) was performed. Of the 102 tested samples, 10 (9.8%) and 7/20 (35%) were detected as positive for the PoSaV RdRp gene using the PCR method at the individual and farm levels, respectively. Genetic analysis of the partial RdRp gene region of about 324 bp indicated that the nucleotide identity of the current 10 Vietnamese viral strains ranged from 61.39% to 100%. Among the 10 strains obtained, 8 belonged to genotype III and the remaining 2 strains were clustered in genotype VIII. The Vietnamese genotype III viruses formed two sub-clusters. The Vietnamese PoSaV strains were closely related to PoSaVs reported in South Korea, Venezuela, and the Netherlands. This research was the first to describe PoSaV infection in northern Vietnam during 2022–2023. |
| first_indexed | 2024-03-11T00:34:16Z |
| format | Article |
| id | doaj.art-790dc628f6824d7fac36ae16272d67c8 |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-11T00:34:16Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-790dc628f6824d7fac36ae16272d67c82023-11-18T21:42:28ZengMDPI AGVeterinary Sciences2306-73812023-07-0110743010.3390/vetsci10070430Porcine Sapovirus in Northern Vietnam: Genetic Detection and Characterization Reveals Co-Circulation of Multiple GenotypesHieu Van Dong0Thai Ha Truong1Giang Thi Huong Tran2Witsanu Rapichai3Amonpun Rattanasrisomporn4Kiattawee Choowongkomon5Jatuporn Rattanasrisomporn6Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University, Bangkok 10900, ThailandFaculty of Veterinary Medicine, Vietnam National University of Agriculture, Trau Quy Town, Gia Lam District, Hanoi 131000, VietnamFaculty of Veterinary Medicine, Vietnam National University of Agriculture, Trau Quy Town, Gia Lam District, Hanoi 131000, VietnamDepartment of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, ThailandInterdisciplinary of Genetic Engineering and Bioinformatics, Graduate School, Kasetsart University, Bangkok 10900, ThailandDepartment of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, ThailandCenter for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University, Bangkok 10900, ThailandPorcine sapovirus (PoSaV) has been reported in many countries over the world, which may cause gastroenteritis symptoms in pigs with all ages. There has been no report on PoSaV infection in Vietnam up to now. In this study, a total of 102 samples were collected from piglets, fattening pigs, and sows with diarrhea in several cities and provinces in northern Vietnam. The PoSaV genome was examined using polymerase chain reaction (PCR). Sequencing of the partial RNA-dependent RNA polymerase (RdRp) gene sequences (324 bp) was performed. Of the 102 tested samples, 10 (9.8%) and 7/20 (35%) were detected as positive for the PoSaV RdRp gene using the PCR method at the individual and farm levels, respectively. Genetic analysis of the partial RdRp gene region of about 324 bp indicated that the nucleotide identity of the current 10 Vietnamese viral strains ranged from 61.39% to 100%. Among the 10 strains obtained, 8 belonged to genotype III and the remaining 2 strains were clustered in genotype VIII. The Vietnamese genotype III viruses formed two sub-clusters. The Vietnamese PoSaV strains were closely related to PoSaVs reported in South Korea, Venezuela, and the Netherlands. This research was the first to describe PoSaV infection in northern Vietnam during 2022–2023.https://www.mdpi.com/2306-7381/10/7/430genotypeporcine sapovirussapoviruspigsVietnam |
| spellingShingle | Hieu Van Dong Thai Ha Truong Giang Thi Huong Tran Witsanu Rapichai Amonpun Rattanasrisomporn Kiattawee Choowongkomon Jatuporn Rattanasrisomporn Porcine Sapovirus in Northern Vietnam: Genetic Detection and Characterization Reveals Co-Circulation of Multiple Genotypes Veterinary Sciences genotype porcine sapovirus sapovirus pigs Vietnam |
| title | Porcine Sapovirus in Northern Vietnam: Genetic Detection and Characterization Reveals Co-Circulation of Multiple Genotypes |
| title_full | Porcine Sapovirus in Northern Vietnam: Genetic Detection and Characterization Reveals Co-Circulation of Multiple Genotypes |
| title_fullStr | Porcine Sapovirus in Northern Vietnam: Genetic Detection and Characterization Reveals Co-Circulation of Multiple Genotypes |
| title_full_unstemmed | Porcine Sapovirus in Northern Vietnam: Genetic Detection and Characterization Reveals Co-Circulation of Multiple Genotypes |
| title_short | Porcine Sapovirus in Northern Vietnam: Genetic Detection and Characterization Reveals Co-Circulation of Multiple Genotypes |
| title_sort | porcine sapovirus in northern vietnam genetic detection and characterization reveals co circulation of multiple genotypes |
| topic | genotype porcine sapovirus sapovirus pigs Vietnam |
| url | https://www.mdpi.com/2306-7381/10/7/430 |
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