Bt, Not a Threat to Propylea japonica
Given the ever-increasing commercial planting of transgenic plants across the world, an evaluation of their impacts on non-target organisms is as an important part of the risk assessment process. Propylea japonica is a dominant non-target predator and pollen feeder insect that is prevalent in Bt cot...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-08-01
|
| Series: | Frontiers in Physiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2020.00758/full |
| _version_ | 1819057641923543040 |
|---|---|
| author | Chenchen Zhao Chenchen Zhao Linke Wu Junyu Luo Lin Niu Lin Niu Chuanpeng Wang Xiangzhen Zhu Li Wang Peng Zhao Shuai Zhang Shuai Zhang Jinjie Cui Jinjie Cui |
| author_facet | Chenchen Zhao Chenchen Zhao Linke Wu Junyu Luo Lin Niu Lin Niu Chuanpeng Wang Xiangzhen Zhu Li Wang Peng Zhao Shuai Zhang Shuai Zhang Jinjie Cui Jinjie Cui |
| author_sort | Chenchen Zhao |
| collection | DOAJ |
| description | Given the ever-increasing commercial planting of transgenic plants across the world, an evaluation of their impacts on non-target organisms is as an important part of the risk assessment process. Propylea japonica is a dominant non-target predator and pollen feeder insect that is prevalent in Bt cotton fields, and it is thus in direct contact with Bt proteins. However, the effect of Bt proteins on P. japonica has not received much attention. In this study, the effects of Cry1Ac and/or Cry2Ab proteins on P. japonica were investigated from three aspects. First, no significant differences in the diversity of the microbiota nor change in species composition and community structure were observed among Cry protein treatments. Firmicutes are the most abundant bacterial phylum present in P. japonica, followed by Proteobacteria and Actinobacteria. The most abundant genus was Staphylococcus. Second, the expression levels of the detoxification and digestion-related genes did not change significantly in any Cry protein treatment. Third, none of the Cry proteins affected the population fitness of P. japonica. These results indicated that P. japonica was not sensitive to Bt proteins, suggesting that growing Bt cotton expressing Cry1Ac and/or Cry2Ab will pose negligible risks to P. japonica. |
| first_indexed | 2024-12-21T13:42:33Z |
| format | Article |
| id | doaj.art-79104924c89745a384a3e0b4a597328f |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-21T13:42:33Z |
| publishDate | 2020-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-79104924c89745a384a3e0b4a597328f2022-12-21T19:01:59ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2020-08-011110.3389/fphys.2020.00758526770Bt, Not a Threat to Propylea japonicaChenchen Zhao0Chenchen Zhao1Linke Wu2Junyu Luo3Lin Niu4Lin Niu5Chuanpeng Wang6Xiangzhen Zhu7Li Wang8Peng Zhao9Shuai Zhang10Shuai Zhang11Jinjie Cui12Jinjie Cui13State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, ChinaHubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, ChinaZhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, ChinaCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, ChinaZhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou, ChinaGiven the ever-increasing commercial planting of transgenic plants across the world, an evaluation of their impacts on non-target organisms is as an important part of the risk assessment process. Propylea japonica is a dominant non-target predator and pollen feeder insect that is prevalent in Bt cotton fields, and it is thus in direct contact with Bt proteins. However, the effect of Bt proteins on P. japonica has not received much attention. In this study, the effects of Cry1Ac and/or Cry2Ab proteins on P. japonica were investigated from three aspects. First, no significant differences in the diversity of the microbiota nor change in species composition and community structure were observed among Cry protein treatments. Firmicutes are the most abundant bacterial phylum present in P. japonica, followed by Proteobacteria and Actinobacteria. The most abundant genus was Staphylococcus. Second, the expression levels of the detoxification and digestion-related genes did not change significantly in any Cry protein treatment. Third, none of the Cry proteins affected the population fitness of P. japonica. These results indicated that P. japonica was not sensitive to Bt proteins, suggesting that growing Bt cotton expressing Cry1Ac and/or Cry2Ab will pose negligible risks to P. japonica.https://www.frontiersin.org/article/10.3389/fphys.2020.00758/fullBt proteinsbacterial diversitydetoxification genesdigestion genesfitness |
| spellingShingle | Chenchen Zhao Chenchen Zhao Linke Wu Junyu Luo Lin Niu Lin Niu Chuanpeng Wang Xiangzhen Zhu Li Wang Peng Zhao Shuai Zhang Shuai Zhang Jinjie Cui Jinjie Cui Bt, Not a Threat to Propylea japonica Frontiers in Physiology Bt proteins bacterial diversity detoxification genes digestion genes fitness |
| title | Bt, Not a Threat to Propylea japonica |
| title_full | Bt, Not a Threat to Propylea japonica |
| title_fullStr | Bt, Not a Threat to Propylea japonica |
| title_full_unstemmed | Bt, Not a Threat to Propylea japonica |
| title_short | Bt, Not a Threat to Propylea japonica |
| title_sort | bt not a threat to propylea japonica |
| topic | Bt proteins bacterial diversity detoxification genes digestion genes fitness |
| url | https://www.frontiersin.org/article/10.3389/fphys.2020.00758/full |
| work_keys_str_mv | AT chenchenzhao btnotathreattopropyleajaponica AT chenchenzhao btnotathreattopropyleajaponica AT linkewu btnotathreattopropyleajaponica AT junyuluo btnotathreattopropyleajaponica AT linniu btnotathreattopropyleajaponica AT linniu btnotathreattopropyleajaponica AT chuanpengwang btnotathreattopropyleajaponica AT xiangzhenzhu btnotathreattopropyleajaponica AT liwang btnotathreattopropyleajaponica AT pengzhao btnotathreattopropyleajaponica AT shuaizhang btnotathreattopropyleajaponica AT shuaizhang btnotathreattopropyleajaponica AT jinjiecui btnotathreattopropyleajaponica AT jinjiecui btnotathreattopropyleajaponica |