Immune-Related LncRNAs Affect the Prognosis of Osteosarcoma, Which Are Related to the Tumor Immune Microenvironment
Background: Abnormal expression of lncRNA is closely related to the occurrence and metastasis of osteosarcoma. The tumor immune microenvironment (TIM) is considered to be an important factor affecting the prognosis and treatment of osteosarcoma. This study aims to explore the effect of immune-relate...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-10-01
|
| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2021.731311/full |
| _version_ | 1818909559559815168 |
|---|---|
| author | Qingshan Huang Qingshan Huang Yilin Lin Chenglong Chen Chenglong Chen Jingbing Lou Jingbing Lou Tingting Ren Tingting Ren Yi Huang Yi Huang Hongliang Zhang Hongliang Zhang Yiyang Yu Yiyang Yu Yu Guo Yu Guo Wei Wang Wei Wang Boyang Wang Boyang Wang Jianfang Niu Jianfang Niu Jiuhui Xu Jiuhui Xu Lei Guo Lei Guo Wei Guo Wei Guo |
| author_facet | Qingshan Huang Qingshan Huang Yilin Lin Chenglong Chen Chenglong Chen Jingbing Lou Jingbing Lou Tingting Ren Tingting Ren Yi Huang Yi Huang Hongliang Zhang Hongliang Zhang Yiyang Yu Yiyang Yu Yu Guo Yu Guo Wei Wang Wei Wang Boyang Wang Boyang Wang Jianfang Niu Jianfang Niu Jiuhui Xu Jiuhui Xu Lei Guo Lei Guo Wei Guo Wei Guo |
| author_sort | Qingshan Huang |
| collection | DOAJ |
| description | Background: Abnormal expression of lncRNA is closely related to the occurrence and metastasis of osteosarcoma. The tumor immune microenvironment (TIM) is considered to be an important factor affecting the prognosis and treatment of osteosarcoma. This study aims to explore the effect of immune-related lncRNAs (IRLs) on the prognosis of osteosarcoma and its relationship with the TIM.Methods: Ninety-five osteosarcoma samples from the TARGET database were included. Iterative LASSO regression and multivariate Cox regression analysis were used to screen the IRLs signature with the optimal AUC. The predict function was used to calculate the risk score and divide osteosarcoma into a high-risk group and low-risk group based on the optimal cut-off value of the risk score. The lncRNAs in IRLs signature that affect metastasis were screened for in vitro validation. Single sample gene set enrichment analysis (ssGSEA) and ESTIMATE algorithms were used to evaluate the role of TIM in the influence of IRLs on osteosarcoma prognosis.Results: Ten IRLs constituted the IRLs signature, with an AUC of 0.96. The recurrence and metastasis rates of osteosarcoma in the high-risk group were higher than those in the low-risk group. In vitro experiments showed that knockdown of lncRNA (AC006033.2) could increase the proliferation, migration, and invasion of osteosarcoma. ssGSEA and ESTIMATE results showed that the immune cell content and immune score in the low-risk group were generally higher than those in the high-risk group. In addition, the expression levels of immune escape-related genes were higher in the high-risk group.Conclusion: The IRLs signature is a reliable biomarker for the prognosis of osteosarcoma, and they alter the prognosis of osteosarcoma. In addition, IRLs signature and patient prognosis may be related to TIM in osteosarcoma. The higher the content of immune cells in the TIM of osteosarcoma, the lower the risk score of patients and the better the prognosis. The higher the expression of immune escape-related genes, the lower the risk score of patients and the better the prognosis. |
| first_indexed | 2024-12-19T22:28:50Z |
| format | Article |
| id | doaj.art-791116e2ab3a4a5ba257a00071310d1c |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-19T22:28:50Z |
| publishDate | 2021-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-791116e2ab3a4a5ba257a00071310d1c2022-12-21T20:03:24ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2021-10-01910.3389/fcell.2021.731311731311Immune-Related LncRNAs Affect the Prognosis of Osteosarcoma, Which Are Related to the Tumor Immune MicroenvironmentQingshan Huang0Qingshan Huang1Yilin Lin2Chenglong Chen3Chenglong Chen4Jingbing Lou5Jingbing Lou6Tingting Ren7Tingting Ren8Yi Huang9Yi Huang10Hongliang Zhang11Hongliang Zhang12Yiyang Yu13Yiyang Yu14Yu Guo15Yu Guo16Wei Wang17Wei Wang18Boyang Wang19Boyang Wang20Jianfang Niu21Jianfang Niu22Jiuhui Xu23Jiuhui Xu24Lei Guo25Lei Guo26Wei Guo27Wei Guo28Musculoskeletal Tumor Center, Peking University People’s Hospital, Beijing, ChinaBeijing Key Laboratory of Musculoskeletal Tumor, Peking University People’s Hospital, Beijing, ChinaLaboratory of Surgical Oncology, Peking University People’s Hospital, Beijing, ChinaMusculoskeletal Tumor Center, Peking University People’s Hospital, Beijing, ChinaBeijing Key Laboratory of Musculoskeletal Tumor, Peking University People’s Hospital, Beijing, ChinaMusculoskeletal Tumor Center, Peking University People’s Hospital, Beijing, ChinaBeijing Key Laboratory of Musculoskeletal Tumor, Peking University People’s Hospital, Beijing, ChinaMusculoskeletal Tumor Center, Peking University People’s Hospital, Beijing, ChinaBeijing Key Laboratory of Musculoskeletal Tumor, Peking University People’s Hospital, Beijing, ChinaMusculoskeletal Tumor Center, Peking University People’s Hospital, Beijing, ChinaBeijing Key Laboratory of Musculoskeletal Tumor, Peking University People’s Hospital, Beijing, ChinaMusculoskeletal Tumor Center, Peking University People’s Hospital, Beijing, ChinaBeijing Key Laboratory of Musculoskeletal Tumor, Peking University People’s Hospital, Beijing, ChinaMusculoskeletal Tumor Center, Peking University People’s Hospital, Beijing, ChinaBeijing Key Laboratory of Musculoskeletal Tumor, Peking University People’s Hospital, Beijing, ChinaMusculoskeletal Tumor Center, Peking University People’s Hospital, Beijing, ChinaBeijing Key Laboratory of Musculoskeletal Tumor, Peking University People’s Hospital, Beijing, ChinaMusculoskeletal Tumor Center, Peking University People’s Hospital, Beijing, ChinaBeijing Key Laboratory of Musculoskeletal Tumor, Peking University People’s Hospital, Beijing, ChinaMusculoskeletal Tumor Center, Peking University People’s Hospital, Beijing, ChinaBeijing Key Laboratory of Musculoskeletal Tumor, Peking University People’s Hospital, Beijing, ChinaMusculoskeletal Tumor Center, Peking University People’s Hospital, Beijing, ChinaBeijing Key Laboratory of Musculoskeletal Tumor, Peking University People’s Hospital, Beijing, ChinaMusculoskeletal Tumor Center, Peking University People’s Hospital, Beijing, ChinaBeijing Key Laboratory of Musculoskeletal Tumor, Peking University People’s Hospital, Beijing, ChinaMusculoskeletal Tumor Center, Peking University People’s Hospital, Beijing, ChinaBeijing Key Laboratory of Musculoskeletal Tumor, Peking University People’s Hospital, Beijing, ChinaMusculoskeletal Tumor Center, Peking University People’s Hospital, Beijing, ChinaBeijing Key Laboratory of Musculoskeletal Tumor, Peking University People’s Hospital, Beijing, ChinaBackground: Abnormal expression of lncRNA is closely related to the occurrence and metastasis of osteosarcoma. The tumor immune microenvironment (TIM) is considered to be an important factor affecting the prognosis and treatment of osteosarcoma. This study aims to explore the effect of immune-related lncRNAs (IRLs) on the prognosis of osteosarcoma and its relationship with the TIM.Methods: Ninety-five osteosarcoma samples from the TARGET database were included. Iterative LASSO regression and multivariate Cox regression analysis were used to screen the IRLs signature with the optimal AUC. The predict function was used to calculate the risk score and divide osteosarcoma into a high-risk group and low-risk group based on the optimal cut-off value of the risk score. The lncRNAs in IRLs signature that affect metastasis were screened for in vitro validation. Single sample gene set enrichment analysis (ssGSEA) and ESTIMATE algorithms were used to evaluate the role of TIM in the influence of IRLs on osteosarcoma prognosis.Results: Ten IRLs constituted the IRLs signature, with an AUC of 0.96. The recurrence and metastasis rates of osteosarcoma in the high-risk group were higher than those in the low-risk group. In vitro experiments showed that knockdown of lncRNA (AC006033.2) could increase the proliferation, migration, and invasion of osteosarcoma. ssGSEA and ESTIMATE results showed that the immune cell content and immune score in the low-risk group were generally higher than those in the high-risk group. In addition, the expression levels of immune escape-related genes were higher in the high-risk group.Conclusion: The IRLs signature is a reliable biomarker for the prognosis of osteosarcoma, and they alter the prognosis of osteosarcoma. In addition, IRLs signature and patient prognosis may be related to TIM in osteosarcoma. The higher the content of immune cells in the TIM of osteosarcoma, the lower the risk score of patients and the better the prognosis. The higher the expression of immune escape-related genes, the lower the risk score of patients and the better the prognosis.https://www.frontiersin.org/articles/10.3389/fcell.2021.731311/fullosteosarcomalncRNAtumor immune microenvironmentimmune escapemetastasis |
| spellingShingle | Qingshan Huang Qingshan Huang Yilin Lin Chenglong Chen Chenglong Chen Jingbing Lou Jingbing Lou Tingting Ren Tingting Ren Yi Huang Yi Huang Hongliang Zhang Hongliang Zhang Yiyang Yu Yiyang Yu Yu Guo Yu Guo Wei Wang Wei Wang Boyang Wang Boyang Wang Jianfang Niu Jianfang Niu Jiuhui Xu Jiuhui Xu Lei Guo Lei Guo Wei Guo Wei Guo Immune-Related LncRNAs Affect the Prognosis of Osteosarcoma, Which Are Related to the Tumor Immune Microenvironment Frontiers in Cell and Developmental Biology osteosarcoma lncRNA tumor immune microenvironment immune escape metastasis |
| title | Immune-Related LncRNAs Affect the Prognosis of Osteosarcoma, Which Are Related to the Tumor Immune Microenvironment |
| title_full | Immune-Related LncRNAs Affect the Prognosis of Osteosarcoma, Which Are Related to the Tumor Immune Microenvironment |
| title_fullStr | Immune-Related LncRNAs Affect the Prognosis of Osteosarcoma, Which Are Related to the Tumor Immune Microenvironment |
| title_full_unstemmed | Immune-Related LncRNAs Affect the Prognosis of Osteosarcoma, Which Are Related to the Tumor Immune Microenvironment |
| title_short | Immune-Related LncRNAs Affect the Prognosis of Osteosarcoma, Which Are Related to the Tumor Immune Microenvironment |
| title_sort | immune related lncrnas affect the prognosis of osteosarcoma which are related to the tumor immune microenvironment |
| topic | osteosarcoma lncRNA tumor immune microenvironment immune escape metastasis |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2021.731311/full |
| work_keys_str_mv | AT qingshanhuang immunerelatedlncrnasaffecttheprognosisofosteosarcomawhicharerelatedtothetumorimmunemicroenvironment AT qingshanhuang immunerelatedlncrnasaffecttheprognosisofosteosarcomawhicharerelatedtothetumorimmunemicroenvironment AT yilinlin immunerelatedlncrnasaffecttheprognosisofosteosarcomawhicharerelatedtothetumorimmunemicroenvironment AT chenglongchen immunerelatedlncrnasaffecttheprognosisofosteosarcomawhicharerelatedtothetumorimmunemicroenvironment AT chenglongchen immunerelatedlncrnasaffecttheprognosisofosteosarcomawhicharerelatedtothetumorimmunemicroenvironment AT jingbinglou immunerelatedlncrnasaffecttheprognosisofosteosarcomawhicharerelatedtothetumorimmunemicroenvironment AT jingbinglou immunerelatedlncrnasaffecttheprognosisofosteosarcomawhicharerelatedtothetumorimmunemicroenvironment AT tingtingren immunerelatedlncrnasaffecttheprognosisofosteosarcomawhicharerelatedtothetumorimmunemicroenvironment AT tingtingren immunerelatedlncrnasaffecttheprognosisofosteosarcomawhicharerelatedtothetumorimmunemicroenvironment AT yihuang immunerelatedlncrnasaffecttheprognosisofosteosarcomawhicharerelatedtothetumorimmunemicroenvironment AT yihuang immunerelatedlncrnasaffecttheprognosisofosteosarcomawhicharerelatedtothetumorimmunemicroenvironment AT hongliangzhang immunerelatedlncrnasaffecttheprognosisofosteosarcomawhicharerelatedtothetumorimmunemicroenvironment AT hongliangzhang immunerelatedlncrnasaffecttheprognosisofosteosarcomawhicharerelatedtothetumorimmunemicroenvironment AT yiyangyu immunerelatedlncrnasaffecttheprognosisofosteosarcomawhicharerelatedtothetumorimmunemicroenvironment AT yiyangyu immunerelatedlncrnasaffecttheprognosisofosteosarcomawhicharerelatedtothetumorimmunemicroenvironment AT yuguo immunerelatedlncrnasaffecttheprognosisofosteosarcomawhicharerelatedtothetumorimmunemicroenvironment AT yuguo immunerelatedlncrnasaffecttheprognosisofosteosarcomawhicharerelatedtothetumorimmunemicroenvironment AT weiwang immunerelatedlncrnasaffecttheprognosisofosteosarcomawhicharerelatedtothetumorimmunemicroenvironment AT weiwang immunerelatedlncrnasaffecttheprognosisofosteosarcomawhicharerelatedtothetumorimmunemicroenvironment AT boyangwang immunerelatedlncrnasaffecttheprognosisofosteosarcomawhicharerelatedtothetumorimmunemicroenvironment AT boyangwang immunerelatedlncrnasaffecttheprognosisofosteosarcomawhicharerelatedtothetumorimmunemicroenvironment AT jianfangniu immunerelatedlncrnasaffecttheprognosisofosteosarcomawhicharerelatedtothetumorimmunemicroenvironment AT jianfangniu immunerelatedlncrnasaffecttheprognosisofosteosarcomawhicharerelatedtothetumorimmunemicroenvironment AT jiuhuixu immunerelatedlncrnasaffecttheprognosisofosteosarcomawhicharerelatedtothetumorimmunemicroenvironment AT jiuhuixu immunerelatedlncrnasaffecttheprognosisofosteosarcomawhicharerelatedtothetumorimmunemicroenvironment AT leiguo immunerelatedlncrnasaffecttheprognosisofosteosarcomawhicharerelatedtothetumorimmunemicroenvironment AT leiguo immunerelatedlncrnasaffecttheprognosisofosteosarcomawhicharerelatedtothetumorimmunemicroenvironment AT weiguo immunerelatedlncrnasaffecttheprognosisofosteosarcomawhicharerelatedtothetumorimmunemicroenvironment AT weiguo immunerelatedlncrnasaffecttheprognosisofosteosarcomawhicharerelatedtothetumorimmunemicroenvironment |