A single-cell atlas of the peripheral immune response in patients with influenza A virus infection
Summary: Influenza A virus (IAV) remains a pressing global health concern, yet our understanding of the specific nature and functional roles of certain circulating cell subsets in relation to this viral infection remains unclear. We performed single-cell RNA sequencing (scRNA-seq) on single-cell who...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223025841 |
| _version_ | 1797388746848468992 |
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| author | Yin Zhang Lu Zong Yuanling Zheng Yu Zhang Nan Li Yaoyao Li Yangyang Jin Liujing Chen Jinling Ouyang Asma Bibi Ying Huang Yuanhong Xu |
| author_facet | Yin Zhang Lu Zong Yuanling Zheng Yu Zhang Nan Li Yaoyao Li Yangyang Jin Liujing Chen Jinling Ouyang Asma Bibi Ying Huang Yuanhong Xu |
| author_sort | Yin Zhang |
| collection | DOAJ |
| description | Summary: Influenza A virus (IAV) remains a pressing global health concern, yet our understanding of the specific nature and functional roles of certain circulating cell subsets in relation to this viral infection remains unclear. We performed single-cell RNA sequencing (scRNA-seq) on single-cell whole-blood (scWB) isolated from various populations using the Singleron Matrix platform. Our investigation showed a significant upregulation of the IFN-stimulated gene, IFN-α-inducible protein 27 (IFI27), in patients affected by IAV infection and further found that the heightened expression of IFI27 was primarily concentrated in specific immune cell populations, including monocytes and conventional dendritic cells (cDCs). Notably, we identified a specific subset of neutrophils, neutrophil_ISG15, which implicates interferon (IFN) signaling in IAV infection. Our findings provide a comprehensive understanding of the cellular subtypes and molecular characteristics of scWB across different populations with IAV infection. |
| first_indexed | 2024-03-08T22:45:17Z |
| format | Article |
| id | doaj.art-7911d457cfdd48058e3e630f49c95953 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-08T22:45:17Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-7911d457cfdd48058e3e630f49c959532023-12-17T06:41:02ZengElsevieriScience2589-00422023-12-012612108507A single-cell atlas of the peripheral immune response in patients with influenza A virus infectionYin Zhang0Lu Zong1Yuanling Zheng2Yu Zhang3Nan Li4Yaoyao Li5Yangyang Jin6Liujing Chen7Jinling Ouyang8Asma Bibi9Ying Huang10Yuanhong Xu11Department of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui Province, China; Department of Pathogen Biology and Provincial Laboratories of Pathogen Biology and Zoonoses, Anhui Medical University, No. 81 Meishan Road, Hefei, ChinaDepartment of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui Province, ChinaDepartment of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui Province, ChinaDepartment of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui Province, ChinaDepartment of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui Province, ChinaDepartment of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui Province, ChinaDepartment of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui Province, ChinaDepartment of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui Province, ChinaDepartment of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui Province, ChinaDepartment of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui Province, China; Department of Pathogen Biology and Provincial Laboratories of Pathogen Biology and Zoonoses, Anhui Medical University, No. 81 Meishan Road, Hefei, ChinaDepartment of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui Province, China; Corresponding authorDepartment of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui Province, China; Department of Pathogen Biology and Provincial Laboratories of Pathogen Biology and Zoonoses, Anhui Medical University, No. 81 Meishan Road, Hefei, China; Corresponding authorSummary: Influenza A virus (IAV) remains a pressing global health concern, yet our understanding of the specific nature and functional roles of certain circulating cell subsets in relation to this viral infection remains unclear. We performed single-cell RNA sequencing (scRNA-seq) on single-cell whole-blood (scWB) isolated from various populations using the Singleron Matrix platform. Our investigation showed a significant upregulation of the IFN-stimulated gene, IFN-α-inducible protein 27 (IFI27), in patients affected by IAV infection and further found that the heightened expression of IFI27 was primarily concentrated in specific immune cell populations, including monocytes and conventional dendritic cells (cDCs). Notably, we identified a specific subset of neutrophils, neutrophil_ISG15, which implicates interferon (IFN) signaling in IAV infection. Our findings provide a comprehensive understanding of the cellular subtypes and molecular characteristics of scWB across different populations with IAV infection.http://www.sciencedirect.com/science/article/pii/S2589004223025841ImmunologyVirologyTranscriptomics |
| spellingShingle | Yin Zhang Lu Zong Yuanling Zheng Yu Zhang Nan Li Yaoyao Li Yangyang Jin Liujing Chen Jinling Ouyang Asma Bibi Ying Huang Yuanhong Xu A single-cell atlas of the peripheral immune response in patients with influenza A virus infection iScience Immunology Virology Transcriptomics |
| title | A single-cell atlas of the peripheral immune response in patients with influenza A virus infection |
| title_full | A single-cell atlas of the peripheral immune response in patients with influenza A virus infection |
| title_fullStr | A single-cell atlas of the peripheral immune response in patients with influenza A virus infection |
| title_full_unstemmed | A single-cell atlas of the peripheral immune response in patients with influenza A virus infection |
| title_short | A single-cell atlas of the peripheral immune response in patients with influenza A virus infection |
| title_sort | single cell atlas of the peripheral immune response in patients with influenza a virus infection |
| topic | Immunology Virology Transcriptomics |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223025841 |
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