Dynamic expression of the pro-dopaminergic transcription factors Pax6 and Dlx2 during postnatal olfactory bulb neurogenesis
Olfactory bulb neurogenesis generates neurons that use GABA, dopamine or glutamine as their neurotransmitters throughout life. Regionalized stem cell populations in the periventricular zone (PVZ) of the lateral ventricles have been shown to be at the basis of neuronal diversity in the system. For ex...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2012-02-01
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| Series: | Frontiers in Cellular Neuroscience |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fncel.2012.00006/full |
| _version_ | 1828491621662982144 |
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| author | Antoine ede Chevigny Nathalie eCore Philipp eFollert Stefan eWild Andreas eBosio Kazuaki eyoshikawa Harold eCremer Christophe eBeclin |
| author_facet | Antoine ede Chevigny Nathalie eCore Philipp eFollert Stefan eWild Andreas eBosio Kazuaki eyoshikawa Harold eCremer Christophe eBeclin |
| author_sort | Antoine ede Chevigny |
| collection | DOAJ |
| description | Olfactory bulb neurogenesis generates neurons that use GABA, dopamine or glutamine as their neurotransmitters throughout life. Regionalized stem cell populations in the periventricular zone (PVZ) of the lateral ventricles have been shown to be at the basis of neuronal diversity in the system. For example dopaminergic neurons arise predominantly from neural stem cells residing in the dorsal PVZ and depend on the expression of the transcription factors Pax6 and Dlx2 for their specification. In addition Dlx2 is required for neurogenesis in general. Using targeted in vivo electroporation combined with immuno-fluorescence imaging and micro-array analysis, we provide here detailed spatial and temporal expression data with cellular resolution in this system. We find that all along the neurogenic process Pax6 expression remains restricted to the dorsal PVZ, whereas nearly all neuroblasts express Dlx2, including those of the dorsal lineage which are switched on for Dlx2 when they enter the RMS. These data allow to explain and precise the functions of these 2 genes in post-natal olfactory bulb neurogenesis. |
| first_indexed | 2024-12-11T11:05:51Z |
| format | Article |
| id | doaj.art-7913fa7b6db740ddad61c866c7aaf4f5 |
| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-12-11T11:05:51Z |
| publishDate | 2012-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-7913fa7b6db740ddad61c866c7aaf4f52022-12-22T01:09:42ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022012-02-01610.3389/fncel.2012.0000621032Dynamic expression of the pro-dopaminergic transcription factors Pax6 and Dlx2 during postnatal olfactory bulb neurogenesisAntoine ede Chevigny0Nathalie eCore1Philipp eFollert2Stefan eWild3Andreas eBosio4Kazuaki eyoshikawa5Harold eCremer6Christophe eBeclin7CNRSCNRSCNRSMiltenyi Biotec GmbHMiltenyi Biotec GmbHOsaka UniversityCNRSCNRSOlfactory bulb neurogenesis generates neurons that use GABA, dopamine or glutamine as their neurotransmitters throughout life. Regionalized stem cell populations in the periventricular zone (PVZ) of the lateral ventricles have been shown to be at the basis of neuronal diversity in the system. For example dopaminergic neurons arise predominantly from neural stem cells residing in the dorsal PVZ and depend on the expression of the transcription factors Pax6 and Dlx2 for their specification. In addition Dlx2 is required for neurogenesis in general. Using targeted in vivo electroporation combined with immuno-fluorescence imaging and micro-array analysis, we provide here detailed spatial and temporal expression data with cellular resolution in this system. We find that all along the neurogenic process Pax6 expression remains restricted to the dorsal PVZ, whereas nearly all neuroblasts express Dlx2, including those of the dorsal lineage which are switched on for Dlx2 when they enter the RMS. These data allow to explain and precise the functions of these 2 genes in post-natal olfactory bulb neurogenesis.http://journal.frontiersin.org/Journal/10.3389/fncel.2012.00006/fullOlfactory BulbPax6Dlx2Subventricular zonein vivo electroporationPost-natal neurogenesis |
| spellingShingle | Antoine ede Chevigny Nathalie eCore Philipp eFollert Stefan eWild Andreas eBosio Kazuaki eyoshikawa Harold eCremer Christophe eBeclin Dynamic expression of the pro-dopaminergic transcription factors Pax6 and Dlx2 during postnatal olfactory bulb neurogenesis Frontiers in Cellular Neuroscience Olfactory Bulb Pax6 Dlx2 Subventricular zone in vivo electroporation Post-natal neurogenesis |
| title | Dynamic expression of the pro-dopaminergic transcription factors Pax6 and Dlx2 during postnatal olfactory bulb neurogenesis |
| title_full | Dynamic expression of the pro-dopaminergic transcription factors Pax6 and Dlx2 during postnatal olfactory bulb neurogenesis |
| title_fullStr | Dynamic expression of the pro-dopaminergic transcription factors Pax6 and Dlx2 during postnatal olfactory bulb neurogenesis |
| title_full_unstemmed | Dynamic expression of the pro-dopaminergic transcription factors Pax6 and Dlx2 during postnatal olfactory bulb neurogenesis |
| title_short | Dynamic expression of the pro-dopaminergic transcription factors Pax6 and Dlx2 during postnatal olfactory bulb neurogenesis |
| title_sort | dynamic expression of the pro dopaminergic transcription factors pax6 and dlx2 during postnatal olfactory bulb neurogenesis |
| topic | Olfactory Bulb Pax6 Dlx2 Subventricular zone in vivo electroporation Post-natal neurogenesis |
| url | http://journal.frontiersin.org/Journal/10.3389/fncel.2012.00006/full |
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