Mapping In Vivo Tumor Oxygenation within Viable Tumor by 19F-MRI and Multispectral Analysis
Quantifying oxygenation in viable tumor remains a major obstacle toward a better understanding of the tumor microenvironment and improving treatment strategies. Current techniques are often complicated by tumor heterogeneity. Herein, a novel in vivo approach that combines 19F magnetic resonance imag...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2013-11-01
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| Series: | Neoplasia: An International Journal for Oncology Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1476558613800851 |
| _version_ | 1811282825342615552 |
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| author | Yunzhou Shi Jason Oeh Jeffrey Eastham-Anderson Sharon Yee David Finkle Franklin V. Peale, Jr Jed Ross Maj Hedehus Nicholas van Bruggen Rayna Venook Sarajane Ross Deepak Sampath Richard A.D. Carano |
| author_facet | Yunzhou Shi Jason Oeh Jeffrey Eastham-Anderson Sharon Yee David Finkle Franklin V. Peale, Jr Jed Ross Maj Hedehus Nicholas van Bruggen Rayna Venook Sarajane Ross Deepak Sampath Richard A.D. Carano |
| author_sort | Yunzhou Shi |
| collection | DOAJ |
| description | Quantifying oxygenation in viable tumor remains a major obstacle toward a better understanding of the tumor microenvironment and improving treatment strategies. Current techniques are often complicated by tumor heterogeneity. Herein, a novel in vivo approach that combines 19F magnetic resonance imaging (19F-MRI)R1 mapping with diffusionbased multispectral (MS) analysis is introduced. This approach restricts the partial pressure of oxygen (pO2) measurements to viable tumor, the tissue of therapeutic interest. The technique exhibited sufficient sensitivity to detect a breathing gas challenge in a xenograft tumor model, and the hypoxic region measured by MS 19F-MRI was strongly correlated with histologic estimates of hypoxia. This approach was then applied to address the effects of antivascular agents on tumor oxygenation, which is a research question that is still under debate. The technique was used to monitor longitudinal pO2 changes in response to an antibody to vascular endothelial growth factor (B20.4.1.1) and a selective dual phosphoinositide 3-kinase/mammalian target of rapamycin inhibitor (GDC-0980). GDC-0980 reduced viable tumor pO2 during a 3-day treatment period, and a significant reduction was also produced by B20.4.1.1. Overall, this method provides an unprecedented view of viable tumor pO2 and contributes to a greater understanding of the effects of antivascular therapies on the tumor's microenvironment. |
| first_indexed | 2024-04-13T01:59:29Z |
| format | Article |
| id | doaj.art-7914fc373e614f65bd3e6d578cf2491d |
| institution | Directory Open Access Journal |
| issn | 1476-5586 1522-8002 |
| language | English |
| last_indexed | 2024-04-13T01:59:29Z |
| publishDate | 2013-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neoplasia: An International Journal for Oncology Research |
| spelling | doaj.art-7914fc373e614f65bd3e6d578cf2491d2022-12-22T03:07:39ZengElsevierNeoplasia: An International Journal for Oncology Research1476-55861522-80022013-11-0115111241125010.1593/neo.131468Mapping In Vivo Tumor Oxygenation within Viable Tumor by 19F-MRI and Multispectral AnalysisYunzhou Shi0Jason Oeh1Jeffrey Eastham-Anderson2Sharon Yee3David Finkle4Franklin V. Peale, Jr5Jed Ross6Maj Hedehus7Nicholas van Bruggen8Rayna Venook9Sarajane Ross10Deepak Sampath11Richard A.D. Carano12Department of Biomedical Imaging, Genentech Inc, South San Francisco, CADepartment of Translational Oncology, Genentech Inc, South San Francisco, CADepartment of Pathology, Genentech Inc, South San Francisco, CADepartment of Translational Oncology, Genentech Inc, South San Francisco, CADepartment of Translational Oncology, Genentech Inc, South San Francisco, CADepartment of Pathology, Genentech Inc, South San Francisco, CADepartment of Biomedical Imaging, Genentech Inc, South San Francisco, CADepartment of Biomedical Imaging, Genentech Inc, South San Francisco, CADepartment of Biomedical Imaging, Genentech Inc, South San Francisco, CADepartment of Translational Oncology, Genentech Inc, South San Francisco, CADepartment of Translational Oncology, Genentech Inc, South San Francisco, CADepartment of Translational Oncology, Genentech Inc, South San Francisco, CADepartment of Biomedical Imaging, Genentech Inc, South San Francisco, CAQuantifying oxygenation in viable tumor remains a major obstacle toward a better understanding of the tumor microenvironment and improving treatment strategies. Current techniques are often complicated by tumor heterogeneity. Herein, a novel in vivo approach that combines 19F magnetic resonance imaging (19F-MRI)R1 mapping with diffusionbased multispectral (MS) analysis is introduced. This approach restricts the partial pressure of oxygen (pO2) measurements to viable tumor, the tissue of therapeutic interest. The technique exhibited sufficient sensitivity to detect a breathing gas challenge in a xenograft tumor model, and the hypoxic region measured by MS 19F-MRI was strongly correlated with histologic estimates of hypoxia. This approach was then applied to address the effects of antivascular agents on tumor oxygenation, which is a research question that is still under debate. The technique was used to monitor longitudinal pO2 changes in response to an antibody to vascular endothelial growth factor (B20.4.1.1) and a selective dual phosphoinositide 3-kinase/mammalian target of rapamycin inhibitor (GDC-0980). GDC-0980 reduced viable tumor pO2 during a 3-day treatment period, and a significant reduction was also produced by B20.4.1.1. Overall, this method provides an unprecedented view of viable tumor pO2 and contributes to a greater understanding of the effects of antivascular therapies on the tumor's microenvironment.http://www.sciencedirect.com/science/article/pii/S1476558613800851 |
| spellingShingle | Yunzhou Shi Jason Oeh Jeffrey Eastham-Anderson Sharon Yee David Finkle Franklin V. Peale, Jr Jed Ross Maj Hedehus Nicholas van Bruggen Rayna Venook Sarajane Ross Deepak Sampath Richard A.D. Carano Mapping In Vivo Tumor Oxygenation within Viable Tumor by 19F-MRI and Multispectral Analysis Neoplasia: An International Journal for Oncology Research |
| title | Mapping In Vivo Tumor Oxygenation within Viable Tumor by 19F-MRI and Multispectral Analysis |
| title_full | Mapping In Vivo Tumor Oxygenation within Viable Tumor by 19F-MRI and Multispectral Analysis |
| title_fullStr | Mapping In Vivo Tumor Oxygenation within Viable Tumor by 19F-MRI and Multispectral Analysis |
| title_full_unstemmed | Mapping In Vivo Tumor Oxygenation within Viable Tumor by 19F-MRI and Multispectral Analysis |
| title_short | Mapping In Vivo Tumor Oxygenation within Viable Tumor by 19F-MRI and Multispectral Analysis |
| title_sort | mapping in vivo tumor oxygenation within viable tumor by 19f mri and multispectral analysis |
| url | http://www.sciencedirect.com/science/article/pii/S1476558613800851 |
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