RECOMBINANT LENTIVIRUS-MEDIATED SILENCING OF ADAPTOR PROTEIN RUK/CIN85 EXPRESSION INFLUENCES BIOLOGICAL RESPONSES OF TUMOR CELLS
Ruk/CIN85 is an adaptor protein that plays important roles in the regulation of cellular processes such as cell death, proliferation and motility. It was recently shown that overexpression of Ruk/CIN85 increases the oncogenic potential of human breast adenocarcinoma MCF-7 cells. It was the aim of th...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2013-08-01
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| Series: | Biotechnologia Acta |
| Subjects: | |
| Online Access: | http://biotechnology.kiev.ua/images/no4_2013/samoylenko_4_2013.pdf |
| _version_ | 1797722704527228928 |
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| author | A. A. Samoylenko N. V. Byts G. V. Pasichnyk N. V. Kozlova A. V. Bazalii D. S. Gerashchenko S. G. Shandrenko A. V. Vorotnikov T. Kietzmann S. V. Komisarenko L. B. Drobot |
| author_facet | A. A. Samoylenko N. V. Byts G. V. Pasichnyk N. V. Kozlova A. V. Bazalii D. S. Gerashchenko S. G. Shandrenko A. V. Vorotnikov T. Kietzmann S. V. Komisarenko L. B. Drobot |
| author_sort | A. A. Samoylenko |
| collection | DOAJ |
| description | Ruk/CIN85 is an adaptor protein that plays important roles in the regulation of cellular processes such as cell death, proliferation and motility. It was recently shown that overexpression of Ruk/CIN85 increases the oncogenic potential of human breast adenocarcinoma MCF-7 cells. It was the aim of the present study to investigate whether inhibition of Ruk/CIN85 expression has an effect on the biological properties of the cells. In order to down-regulate Ruk/CIN85 expression of small interfering RNA-based approach was used. For down-regulation of Ruk/CIN85 lentiviral constructs encoding Ruk/CIN85-specific small hairpin RNA sequences were generated. By using the obtained recombinant lentiviruses it was shown that inhibition of Ruk/CIN85 expression influences biological properties (motility, proliferation, ABCG2 expression, and ROS generation) of various tumour cell types such as human breast adenocarcinoma MCF-7, human colorectal adenocarcinoma HT-29, and Lewis mouse lung carcinoma cells. |
| first_indexed | 2024-03-12T09:52:16Z |
| format | Article |
| id | doaj.art-791716ead39e4554b4d2e38462099f29 |
| institution | Directory Open Access Journal |
| issn | 2410-7751 2410-776X |
| language | English |
| last_indexed | 2024-03-12T09:52:16Z |
| publishDate | 2013-08-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | Biotechnologia Acta |
| spelling | doaj.art-791716ead39e4554b4d2e38462099f292023-09-02T12:26:23ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryBiotechnologia Acta2410-77512410-776X2013-08-016418218910.15407/biotech6.04.182RECOMBINANT LENTIVIRUS-MEDIATED SILENCING OF ADAPTOR PROTEIN RUK/CIN85 EXPRESSION INFLUENCES BIOLOGICAL RESPONSES OF TUMOR CELLSA. A. Samoylenko0N. V. Byts1G. V. Pasichnyk2N. V. Kozlova3A. V. Bazalii4D. S. Gerashchenko5 S. G. Shandrenko6A. V. Vorotnikov7T. Kietzmann8S. V. Komisarenko9L. B. Drobot10Palladian Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv, UkrainePalladian Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv, UkrainePalladian Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv, UkrainePalladian Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv, UkrainePalladian Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv, UkrainePalladian Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv, UkrainePalladian Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv, UkraineFaculty of Fundamental Medicine, Lomonosov Moscow State University, Moscow, RussiaDepartment of Biochemistry and Biocenter Oulu, University of Oulu, Oulu, FinlandPalladian Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv, UkrainePalladian Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv, UkraineRuk/CIN85 is an adaptor protein that plays important roles in the regulation of cellular processes such as cell death, proliferation and motility. It was recently shown that overexpression of Ruk/CIN85 increases the oncogenic potential of human breast adenocarcinoma MCF-7 cells. It was the aim of the present study to investigate whether inhibition of Ruk/CIN85 expression has an effect on the biological properties of the cells. In order to down-regulate Ruk/CIN85 expression of small interfering RNA-based approach was used. For down-regulation of Ruk/CIN85 lentiviral constructs encoding Ruk/CIN85-specific small hairpin RNA sequences were generated. By using the obtained recombinant lentiviruses it was shown that inhibition of Ruk/CIN85 expression influences biological properties (motility, proliferation, ABCG2 expression, and ROS generation) of various tumour cell types such as human breast adenocarcinoma MCF-7, human colorectal adenocarcinoma HT-29, and Lewis mouse lung carcinoma cells.http://biotechnology.kiev.ua/images/no4_2013/samoylenko_4_2013.pdfadapter proteinsRuk/CIN85RNAinterferencelentiviral vectorscell motilityactive oxygen forms |
| spellingShingle | A. A. Samoylenko N. V. Byts G. V. Pasichnyk N. V. Kozlova A. V. Bazalii D. S. Gerashchenko S. G. Shandrenko A. V. Vorotnikov T. Kietzmann S. V. Komisarenko L. B. Drobot RECOMBINANT LENTIVIRUS-MEDIATED SILENCING OF ADAPTOR PROTEIN RUK/CIN85 EXPRESSION INFLUENCES BIOLOGICAL RESPONSES OF TUMOR CELLS Biotechnologia Acta adapter proteins Ruk/CIN85 RNAinterference lentiviral vectors cell motility active oxygen forms |
| title | RECOMBINANT LENTIVIRUS-MEDIATED SILENCING OF ADAPTOR PROTEIN RUK/CIN85 EXPRESSION INFLUENCES BIOLOGICAL RESPONSES OF TUMOR CELLS |
| title_full | RECOMBINANT LENTIVIRUS-MEDIATED SILENCING OF ADAPTOR PROTEIN RUK/CIN85 EXPRESSION INFLUENCES BIOLOGICAL RESPONSES OF TUMOR CELLS |
| title_fullStr | RECOMBINANT LENTIVIRUS-MEDIATED SILENCING OF ADAPTOR PROTEIN RUK/CIN85 EXPRESSION INFLUENCES BIOLOGICAL RESPONSES OF TUMOR CELLS |
| title_full_unstemmed | RECOMBINANT LENTIVIRUS-MEDIATED SILENCING OF ADAPTOR PROTEIN RUK/CIN85 EXPRESSION INFLUENCES BIOLOGICAL RESPONSES OF TUMOR CELLS |
| title_short | RECOMBINANT LENTIVIRUS-MEDIATED SILENCING OF ADAPTOR PROTEIN RUK/CIN85 EXPRESSION INFLUENCES BIOLOGICAL RESPONSES OF TUMOR CELLS |
| title_sort | recombinant lentivirus mediated silencing of adaptor protein ruk cin85 expression influences biological responses of tumor cells |
| topic | adapter proteins Ruk/CIN85 RNAinterference lentiviral vectors cell motility active oxygen forms |
| url | http://biotechnology.kiev.ua/images/no4_2013/samoylenko_4_2013.pdf |
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