Molecular Screening of Microorganisms Associated with Discolored Wood in Dead European Beech Trees Suffered from Extreme Drought Event Using Next Generation Sequencing
Drought events weaken trees and make them vulnerable to attacks by diverse plant pathogens. Here, we propose a molecular method for fast screening of microorganisms associated with European beech decline after an extreme drought period (2018) in a forest of Thuringia, Germany. We used Illumina seque...
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MDPI AG
2021-10-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/10/2092 |
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| author | Witoon Purahong Benjawan Tanunchai Sara Fareed Mohamed Wahdan François Buscot Ernst-Detlef Schulze |
| author_facet | Witoon Purahong Benjawan Tanunchai Sara Fareed Mohamed Wahdan François Buscot Ernst-Detlef Schulze |
| author_sort | Witoon Purahong |
| collection | DOAJ |
| description | Drought events weaken trees and make them vulnerable to attacks by diverse plant pathogens. Here, we propose a molecular method for fast screening of microorganisms associated with European beech decline after an extreme drought period (2018) in a forest of Thuringia, Germany. We used Illumina sequencing with a recent bioinformatics approach based on DADA2 to identify archaeal, bacterial, and fungal ASVs (amplicon sequence variants) based on bacterial and archaeal 16S and fungal ITS genes. We show that symptomatic beech trees are associated with both bacterial and fungal plant pathogens. Although the plant pathogen sequences were detected in both discolored and non-discolored wood areas, they were highly enriched in the discolored wood areas. We show that almost each individual tree was associated with a different combination of pathogens. <i>Cytospora</i> spp. and <i>Neonectria coccinea</i> were among the most frequently detected fungal pathogens, whereas <i>Erwinia</i> spp. and <i>Pseudomonas</i> spp. were the dominant bacterial plant pathogens. We demonstrate that bacterial plant pathogens may be of major importance in beech decline. |
| first_indexed | 2024-03-10T06:15:20Z |
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| id | doaj.art-7917a2cca8f94a57a0bde9671339800b |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T06:15:20Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-7917a2cca8f94a57a0bde9671339800b2023-11-22T19:43:38ZengMDPI AGPlants2223-77472021-10-011010209210.3390/plants10102092Molecular Screening of Microorganisms Associated with Discolored Wood in Dead European Beech Trees Suffered from Extreme Drought Event Using Next Generation SequencingWitoon Purahong0Benjawan Tanunchai1Sara Fareed Mohamed Wahdan2François Buscot3Ernst-Detlef Schulze4UFZ-Helmholtz Centre for Environmental Research, Department of Soil Ecology, Theodor-Lieser-Str. 4, D-06120 Halle, GermanyUFZ-Helmholtz Centre for Environmental Research, Department of Soil Ecology, Theodor-Lieser-Str. 4, D-06120 Halle, GermanyUFZ-Helmholtz Centre for Environmental Research, Department of Soil Ecology, Theodor-Lieser-Str. 4, D-06120 Halle, GermanyUFZ-Helmholtz Centre for Environmental Research, Department of Soil Ecology, Theodor-Lieser-Str. 4, D-06120 Halle, GermanyMax Planck Institute for Biogeochemistry, Hans-Knöll-Str. 10, D-07745 Jena, GermanyDrought events weaken trees and make them vulnerable to attacks by diverse plant pathogens. Here, we propose a molecular method for fast screening of microorganisms associated with European beech decline after an extreme drought period (2018) in a forest of Thuringia, Germany. We used Illumina sequencing with a recent bioinformatics approach based on DADA2 to identify archaeal, bacterial, and fungal ASVs (amplicon sequence variants) based on bacterial and archaeal 16S and fungal ITS genes. We show that symptomatic beech trees are associated with both bacterial and fungal plant pathogens. Although the plant pathogen sequences were detected in both discolored and non-discolored wood areas, they were highly enriched in the discolored wood areas. We show that almost each individual tree was associated with a different combination of pathogens. <i>Cytospora</i> spp. and <i>Neonectria coccinea</i> were among the most frequently detected fungal pathogens, whereas <i>Erwinia</i> spp. and <i>Pseudomonas</i> spp. were the dominant bacterial plant pathogens. We demonstrate that bacterial plant pathogens may be of major importance in beech decline.https://www.mdpi.com/2223-7747/10/10/2092climate change<i>Fagus sylvatica</i>Illumina sequencingmeta-barcodingplant pathogenic bacteriaplant pathogenic fungi |
| spellingShingle | Witoon Purahong Benjawan Tanunchai Sara Fareed Mohamed Wahdan François Buscot Ernst-Detlef Schulze Molecular Screening of Microorganisms Associated with Discolored Wood in Dead European Beech Trees Suffered from Extreme Drought Event Using Next Generation Sequencing Plants climate change <i>Fagus sylvatica</i> Illumina sequencing meta-barcoding plant pathogenic bacteria plant pathogenic fungi |
| title | Molecular Screening of Microorganisms Associated with Discolored Wood in Dead European Beech Trees Suffered from Extreme Drought Event Using Next Generation Sequencing |
| title_full | Molecular Screening of Microorganisms Associated with Discolored Wood in Dead European Beech Trees Suffered from Extreme Drought Event Using Next Generation Sequencing |
| title_fullStr | Molecular Screening of Microorganisms Associated with Discolored Wood in Dead European Beech Trees Suffered from Extreme Drought Event Using Next Generation Sequencing |
| title_full_unstemmed | Molecular Screening of Microorganisms Associated with Discolored Wood in Dead European Beech Trees Suffered from Extreme Drought Event Using Next Generation Sequencing |
| title_short | Molecular Screening of Microorganisms Associated with Discolored Wood in Dead European Beech Trees Suffered from Extreme Drought Event Using Next Generation Sequencing |
| title_sort | molecular screening of microorganisms associated with discolored wood in dead european beech trees suffered from extreme drought event using next generation sequencing |
| topic | climate change <i>Fagus sylvatica</i> Illumina sequencing meta-barcoding plant pathogenic bacteria plant pathogenic fungi |
| url | https://www.mdpi.com/2223-7747/10/10/2092 |
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