Comprehensive in-silico characterization and expression analysis of UbiA prenyltransferase genes in peanut (Arachis hypogaea L.) against abiotic stresses
Genes of the UbiA prenyltransferase family play key roles in secondary metabolites synthesis and biotic and abiotic stress tolerance through different metabolic pathways. Currently, there is a notable lack of comprehensive information on the UbiA genes in legumes, particularly in peanuts. To bridge...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-12-01
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| Series: | Plant Stress |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667064X23000969 |
| _version_ | 1827609501238296576 |
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| author | Qiang Yang Yasir Sharif Yuhui Zhuang Tiecheng Cai Lihui Wang Huiwen Fu Wenzhi Lu Min Ma Huan Yang Huaqi Li Ali Raza Chong Zhang Hua Chen Faqian Xiong Weijian Zhuang |
| author_facet | Qiang Yang Yasir Sharif Yuhui Zhuang Tiecheng Cai Lihui Wang Huiwen Fu Wenzhi Lu Min Ma Huan Yang Huaqi Li Ali Raza Chong Zhang Hua Chen Faqian Xiong Weijian Zhuang |
| author_sort | Qiang Yang |
| collection | DOAJ |
| description | Genes of the UbiA prenyltransferase family play key roles in secondary metabolites synthesis and biotic and abiotic stress tolerance through different metabolic pathways. Currently, there is a notable lack of comprehensive information on the UbiA genes in legumes, particularly in peanuts. To bridge this gap, we conducted a detailed genome-wide analysis to identify the UbiA genes in the peanut genome. We studied chromosomal organization, gene structure, phylogenetic relations, protein interactions, collinearity, and gene duplication events. Additionally, we predicted different cis-elements in UbiA promoters and miRNAs target sites, while expression matrices in different organs and stresses also were examined. Our study found 43 UbiA members in the peanut genome (AhUbiA), clustered into seven distinct phylogenetic groups. Tandem and segmental duplication played significant roles in the evolution of AhUbiA genes. AhUbiA promoters were enriched in key cis-elements, including 'growth and development', 'phytohormones', 'light induction', and 'defense-related' elements. Micro-RNAs from five different families targeted 12 AhUbiA genes. GO enrichment analysis indicated that AhUbiA proteins are highly enriched in transferase and catalytic activities, cellular metabolic, and biosynthesis processes. AhUbiA genes recorded expression variance in different tissues. AhUbiA-15 and AhUbiA-38 were highly expressed in roots and root nodules. AhUbiA-6, AhUbiA-7, and AhUbiA-10 were highly expressed under stress conditions. These findings are valuable for future functional studies on the AhUbiA genes for peanut breeding programs. |
| first_indexed | 2024-03-09T07:33:18Z |
| format | Article |
| id | doaj.art-7919a9a1f08744948ac4823899d24322 |
| institution | Directory Open Access Journal |
| issn | 2667-064X |
| language | English |
| last_indexed | 2024-03-09T07:33:18Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Plant Stress |
| spelling | doaj.art-7919a9a1f08744948ac4823899d243222023-12-03T05:43:29ZengElsevierPlant Stress2667-064X2023-12-0110100229Comprehensive in-silico characterization and expression analysis of UbiA prenyltransferase genes in peanut (Arachis hypogaea L.) against abiotic stressesQiang Yang0Yasir Sharif1Yuhui Zhuang2Tiecheng Cai3Lihui Wang4Huiwen Fu5Wenzhi Lu6Min Ma7Huan Yang8Huaqi Li9Ali Raza10Chong Zhang11Hua Chen12Faqian Xiong13Weijian Zhuang14Center of Legume Plant Genetics and Systems Biology, College of Agronomy, Fujian Agriculture and Forestry University (FAFU), Fuzhou, Fujian 350002, ChinaCenter of Legume Plant Genetics and Systems Biology, College of Agronomy, Fujian Agriculture and Forestry University (FAFU), Fuzhou, Fujian 350002, ChinaCollege of Life Science, Fujian Agriculture and Forestry University (FAFU), Fuzhou, Fujian 350002, ChinaCenter of Legume Plant Genetics and Systems Biology, College of Agronomy, Fujian Agriculture and Forestry University (FAFU), Fuzhou, Fujian 350002, ChinaCollege of Plant Protection, Fujian Agriculture and Forestry University (FAFU), Fuzhou, Fujian 350002, ChinaCollege of Plant Protection, Fujian Agriculture and Forestry University (FAFU), Fuzhou, Fujian 350002, ChinaCenter of Legume Plant Genetics and Systems Biology, College of Agronomy, Fujian Agriculture and Forestry University (FAFU), Fuzhou, Fujian 350002, ChinaCenter of Legume Plant Genetics and Systems Biology, College of Agronomy, Fujian Agriculture and Forestry University (FAFU), Fuzhou, Fujian 350002, ChinaCenter of Legume Plant Genetics and Systems Biology, College of Agronomy, Fujian Agriculture and Forestry University (FAFU), Fuzhou, Fujian 350002, ChinaCollege of Plant Protection, Fujian Agriculture and Forestry University (FAFU), Fuzhou, Fujian 350002, ChinaCenter of Legume Plant Genetics and Systems Biology, College of Agronomy, Fujian Agriculture and Forestry University (FAFU), Fuzhou, Fujian 350002, ChinaCenter of Legume Plant Genetics and Systems Biology, College of Agronomy, Fujian Agriculture and Forestry University (FAFU), Fuzhou, Fujian 350002, ChinaCenter of Legume Plant Genetics and Systems Biology, College of Agronomy, Fujian Agriculture and Forestry University (FAFU), Fuzhou, Fujian 350002, ChinaSugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China; Corresponding authors.Center of Legume Plant Genetics and Systems Biology, College of Agronomy, Fujian Agriculture and Forestry University (FAFU), Fuzhou, Fujian 350002, China; Corresponding authors.Genes of the UbiA prenyltransferase family play key roles in secondary metabolites synthesis and biotic and abiotic stress tolerance through different metabolic pathways. Currently, there is a notable lack of comprehensive information on the UbiA genes in legumes, particularly in peanuts. To bridge this gap, we conducted a detailed genome-wide analysis to identify the UbiA genes in the peanut genome. We studied chromosomal organization, gene structure, phylogenetic relations, protein interactions, collinearity, and gene duplication events. Additionally, we predicted different cis-elements in UbiA promoters and miRNAs target sites, while expression matrices in different organs and stresses also were examined. Our study found 43 UbiA members in the peanut genome (AhUbiA), clustered into seven distinct phylogenetic groups. Tandem and segmental duplication played significant roles in the evolution of AhUbiA genes. AhUbiA promoters were enriched in key cis-elements, including 'growth and development', 'phytohormones', 'light induction', and 'defense-related' elements. Micro-RNAs from five different families targeted 12 AhUbiA genes. GO enrichment analysis indicated that AhUbiA proteins are highly enriched in transferase and catalytic activities, cellular metabolic, and biosynthesis processes. AhUbiA genes recorded expression variance in different tissues. AhUbiA-15 and AhUbiA-38 were highly expressed in roots and root nodules. AhUbiA-6, AhUbiA-7, and AhUbiA-10 were highly expressed under stress conditions. These findings are valuable for future functional studies on the AhUbiA genes for peanut breeding programs.http://www.sciencedirect.com/science/article/pii/S2667064X23000969Agricultural sustainabilityEnvironmental stressFood securityGene duplicationStress tolerancePeanut genetics |
| spellingShingle | Qiang Yang Yasir Sharif Yuhui Zhuang Tiecheng Cai Lihui Wang Huiwen Fu Wenzhi Lu Min Ma Huan Yang Huaqi Li Ali Raza Chong Zhang Hua Chen Faqian Xiong Weijian Zhuang Comprehensive in-silico characterization and expression analysis of UbiA prenyltransferase genes in peanut (Arachis hypogaea L.) against abiotic stresses Plant Stress Agricultural sustainability Environmental stress Food security Gene duplication Stress tolerance Peanut genetics |
| title | Comprehensive in-silico characterization and expression analysis of UbiA prenyltransferase genes in peanut (Arachis hypogaea L.) against abiotic stresses |
| title_full | Comprehensive in-silico characterization and expression analysis of UbiA prenyltransferase genes in peanut (Arachis hypogaea L.) against abiotic stresses |
| title_fullStr | Comprehensive in-silico characterization and expression analysis of UbiA prenyltransferase genes in peanut (Arachis hypogaea L.) against abiotic stresses |
| title_full_unstemmed | Comprehensive in-silico characterization and expression analysis of UbiA prenyltransferase genes in peanut (Arachis hypogaea L.) against abiotic stresses |
| title_short | Comprehensive in-silico characterization and expression analysis of UbiA prenyltransferase genes in peanut (Arachis hypogaea L.) against abiotic stresses |
| title_sort | comprehensive in silico characterization and expression analysis of ubia prenyltransferase genes in peanut arachis hypogaea l against abiotic stresses |
| topic | Agricultural sustainability Environmental stress Food security Gene duplication Stress tolerance Peanut genetics |
| url | http://www.sciencedirect.com/science/article/pii/S2667064X23000969 |
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