Autophagy Involvement in the Postnatal Development of the Rat Retina
During retinal development, a physiologic hypoxia stimulates endothelial cell proliferation. The hypoxic milieu warrants retina vascularization and promotes the activation of several mechanisms aimed to ensure homeostasis and energy balance of both endothelial and retinal cells. Autophagy is an evol...
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MDPI AG
2021-01-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/10/1/177 |
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| author | Noemi Anna Pesce Alessio Canovai Emma Lardner Maurizio Cammalleri Anders Kvanta Helder André Massimo Dal Monte |
| author_facet | Noemi Anna Pesce Alessio Canovai Emma Lardner Maurizio Cammalleri Anders Kvanta Helder André Massimo Dal Monte |
| author_sort | Noemi Anna Pesce |
| collection | DOAJ |
| description | During retinal development, a physiologic hypoxia stimulates endothelial cell proliferation. The hypoxic milieu warrants retina vascularization and promotes the activation of several mechanisms aimed to ensure homeostasis and energy balance of both endothelial and retinal cells. Autophagy is an evolutionarily conserved catabolic system that contributes to cellular adaptation to a variety of environmental changes and stresses. In association with the physiologic hypoxia, autophagy plays a crucial role during development. Autophagy expression profile was evaluated in the developing retina from birth to post-natal day 18 of rat pups, using qPCR, western blotting and immunostaining methodologies. The rat post-partum developing retina displayed increased active autophagy during the first postnatal days, correlating to the hypoxic phase. In latter stages of development, rat retinal autophagy decreases, reaching a normalization between post-natal days 14-18, when the retina is fully vascularized and mature. Collectively, the present study elaborates on the link between hypoxia and autophagy, and contributes to further elucidate the role of autophagy during retinal development. |
| first_indexed | 2024-03-09T04:32:58Z |
| format | Article |
| id | doaj.art-791ce60accb944139b0ca35f37a54c6a |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T04:32:58Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
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| series | Cells |
| spelling | doaj.art-791ce60accb944139b0ca35f37a54c6a2023-12-03T13:33:23ZengMDPI AGCells2073-44092021-01-0110117710.3390/cells10010177Autophagy Involvement in the Postnatal Development of the Rat RetinaNoemi Anna Pesce0Alessio Canovai1Emma Lardner2Maurizio Cammalleri3Anders Kvanta4Helder André5Massimo Dal Monte6Department of Clinical Neuroscience, Division of Eye and Vision, St. Erik Eye Hospital, Karolinska Institutet, Eugeniavägen 12, 17164 Solna, SwedenDepartment of Biology, University of Pisa, Via San Zeno 31, 56127 Pisa, ItalyDepartment of Clinical Neuroscience, Division of Eye and Vision, St. Erik Eye Hospital, Karolinska Institutet, Eugeniavägen 12, 17164 Solna, SwedenDepartment of Biology, University of Pisa, Via San Zeno 31, 56127 Pisa, ItalyDepartment of Clinical Neuroscience, Division of Eye and Vision, St. Erik Eye Hospital, Karolinska Institutet, Eugeniavägen 12, 17164 Solna, SwedenDepartment of Clinical Neuroscience, Division of Eye and Vision, St. Erik Eye Hospital, Karolinska Institutet, Eugeniavägen 12, 17164 Solna, SwedenDepartment of Biology, University of Pisa, Via San Zeno 31, 56127 Pisa, ItalyDuring retinal development, a physiologic hypoxia stimulates endothelial cell proliferation. The hypoxic milieu warrants retina vascularization and promotes the activation of several mechanisms aimed to ensure homeostasis and energy balance of both endothelial and retinal cells. Autophagy is an evolutionarily conserved catabolic system that contributes to cellular adaptation to a variety of environmental changes and stresses. In association with the physiologic hypoxia, autophagy plays a crucial role during development. Autophagy expression profile was evaluated in the developing retina from birth to post-natal day 18 of rat pups, using qPCR, western blotting and immunostaining methodologies. The rat post-partum developing retina displayed increased active autophagy during the first postnatal days, correlating to the hypoxic phase. In latter stages of development, rat retinal autophagy decreases, reaching a normalization between post-natal days 14-18, when the retina is fully vascularized and mature. Collectively, the present study elaborates on the link between hypoxia and autophagy, and contributes to further elucidate the role of autophagy during retinal development.https://www.mdpi.com/2073-4409/10/1/177eyeretinadevelopmentvascularizationhypoxiaautophagy |
| spellingShingle | Noemi Anna Pesce Alessio Canovai Emma Lardner Maurizio Cammalleri Anders Kvanta Helder André Massimo Dal Monte Autophagy Involvement in the Postnatal Development of the Rat Retina Cells eye retina development vascularization hypoxia autophagy |
| title | Autophagy Involvement in the Postnatal Development of the Rat Retina |
| title_full | Autophagy Involvement in the Postnatal Development of the Rat Retina |
| title_fullStr | Autophagy Involvement in the Postnatal Development of the Rat Retina |
| title_full_unstemmed | Autophagy Involvement in the Postnatal Development of the Rat Retina |
| title_short | Autophagy Involvement in the Postnatal Development of the Rat Retina |
| title_sort | autophagy involvement in the postnatal development of the rat retina |
| topic | eye retina development vascularization hypoxia autophagy |
| url | https://www.mdpi.com/2073-4409/10/1/177 |
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