miR-1183 Is a Key Marker of Remodeling upon Stretch and Tachycardia in Human Myocardium
Many cardiac insults causing atrial remodeling are linked to either stretch or tachycardia, but a comparative characterization of their effects on early remodeling events in human myocardium is lacking. Here, we applied isometric stretch or sustained tachycardia at 2.5 Hz in human atrial trabeculae...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/13/6962 |
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| author | Natasa Djalinac Ewald Kolesnik Heinrich Maechler Susanne Scheruebel-Posch Brigitte Pelzmann Peter P. Rainer Ines Foessl Markus Wallner Daniel Scherr Akos Heinemann Simon Sedej Senka Ljubojevic-Holzer Dirk von Lewinski Egbert Bisping |
| author_facet | Natasa Djalinac Ewald Kolesnik Heinrich Maechler Susanne Scheruebel-Posch Brigitte Pelzmann Peter P. Rainer Ines Foessl Markus Wallner Daniel Scherr Akos Heinemann Simon Sedej Senka Ljubojevic-Holzer Dirk von Lewinski Egbert Bisping |
| author_sort | Natasa Djalinac |
| collection | DOAJ |
| description | Many cardiac insults causing atrial remodeling are linked to either stretch or tachycardia, but a comparative characterization of their effects on early remodeling events in human myocardium is lacking. Here, we applied isometric stretch or sustained tachycardia at 2.5 Hz in human atrial trabeculae for 6 h followed by microarray gene expression profiling. Among largely independent expression patterns, we found a small common fraction with the microRNA miR-1183 as the highest up-regulated transcript (up to 4-fold). Both, acute stretch and tachycardia induced down-regulation of the predicted miR-1183 target genes <i>ADAM20</i> and <i>PLA2G7</i>. Furthermore, miR-1183 was also significantly up-regulated in chronically remodeled atrial samples from patients with persistent atrial fibrillation (3-fold up-regulation versus sinus rhythm samples), and in ventricular myocardium from dilative cardiomyopathy hearts (2-fold up-regulation) as compared to non-failing controls. In sum, although stretch and tachycardia show distinct transcriptomic signatures in human atrial myocardium, both cardiac insults consistently regulate the expression of miR-1183 and its downstream targets in acute and chronic remodeling. Thus, elevated expression of miR-1183 might serve as a tissue biomarker for atrial remodeling and might be of potential functional significance in cardiac disease. |
| first_indexed | 2024-03-09T21:51:55Z |
| format | Article |
| id | doaj.art-791e2877ef0b43d49dd3176269973718 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:51:55Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-791e2877ef0b43d49dd31762699737182023-11-23T20:05:32ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-06-012313696210.3390/ijms23136962miR-1183 Is a Key Marker of Remodeling upon Stretch and Tachycardia in Human MyocardiumNatasa Djalinac0Ewald Kolesnik1Heinrich Maechler2Susanne Scheruebel-Posch3Brigitte Pelzmann4Peter P. Rainer5Ines Foessl6Markus Wallner7Daniel Scherr8Akos Heinemann9Simon Sedej10Senka Ljubojevic-Holzer11Dirk von Lewinski12Egbert Bisping13Department of Internal Medicine, Division of Cardiology, Medical University of Graz, 8036 Graz, AustriaDepartment of Internal Medicine, Division of Cardiology, Medical University of Graz, 8036 Graz, AustriaDepartment of Cardiothoracic Surgery, Medical University of Graz, 8036 Graz, AustriaGottfried Schatz Research Center, Institute of Biophysics, Medical University of Graz, 8010 Graz, AustriaGottfried Schatz Research Center, Institute of Biophysics, Medical University of Graz, 8010 Graz, AustriaDepartment of Internal Medicine, Division of Cardiology, Medical University of Graz, 8036 Graz, AustriaDepartment of Internal Medicine, Division of Endocrinology and Diabetology, Medical University of Graz, 8010 Graz, AustriaDepartment of Internal Medicine, Division of Cardiology, Medical University of Graz, 8036 Graz, AustriaDepartment of Internal Medicine, Division of Cardiology, Medical University of Graz, 8036 Graz, AustriaInstitute of Experimental and Clinical Pharmacology, Medical University of Graz, 8010 Graz, AustriaDepartment of Internal Medicine, Division of Cardiology, Medical University of Graz, 8036 Graz, AustriaDepartment of Internal Medicine, Division of Cardiology, Medical University of Graz, 8036 Graz, AustriaDepartment of Internal Medicine, Division of Cardiology, Medical University of Graz, 8036 Graz, AustriaDepartment of Internal Medicine, Division of Cardiology, Medical University of Graz, 8036 Graz, AustriaMany cardiac insults causing atrial remodeling are linked to either stretch or tachycardia, but a comparative characterization of their effects on early remodeling events in human myocardium is lacking. Here, we applied isometric stretch or sustained tachycardia at 2.5 Hz in human atrial trabeculae for 6 h followed by microarray gene expression profiling. Among largely independent expression patterns, we found a small common fraction with the microRNA miR-1183 as the highest up-regulated transcript (up to 4-fold). Both, acute stretch and tachycardia induced down-regulation of the predicted miR-1183 target genes <i>ADAM20</i> and <i>PLA2G7</i>. Furthermore, miR-1183 was also significantly up-regulated in chronically remodeled atrial samples from patients with persistent atrial fibrillation (3-fold up-regulation versus sinus rhythm samples), and in ventricular myocardium from dilative cardiomyopathy hearts (2-fold up-regulation) as compared to non-failing controls. In sum, although stretch and tachycardia show distinct transcriptomic signatures in human atrial myocardium, both cardiac insults consistently regulate the expression of miR-1183 and its downstream targets in acute and chronic remodeling. Thus, elevated expression of miR-1183 might serve as a tissue biomarker for atrial remodeling and might be of potential functional significance in cardiac disease.https://www.mdpi.com/1422-0067/23/13/6962stretchtachycardiacardiac remodelingmicroRNA<i>MIR1183</i>miR-1183 |
| spellingShingle | Natasa Djalinac Ewald Kolesnik Heinrich Maechler Susanne Scheruebel-Posch Brigitte Pelzmann Peter P. Rainer Ines Foessl Markus Wallner Daniel Scherr Akos Heinemann Simon Sedej Senka Ljubojevic-Holzer Dirk von Lewinski Egbert Bisping miR-1183 Is a Key Marker of Remodeling upon Stretch and Tachycardia in Human Myocardium International Journal of Molecular Sciences stretch tachycardia cardiac remodeling microRNA <i>MIR1183</i> miR-1183 |
| title | miR-1183 Is a Key Marker of Remodeling upon Stretch and Tachycardia in Human Myocardium |
| title_full | miR-1183 Is a Key Marker of Remodeling upon Stretch and Tachycardia in Human Myocardium |
| title_fullStr | miR-1183 Is a Key Marker of Remodeling upon Stretch and Tachycardia in Human Myocardium |
| title_full_unstemmed | miR-1183 Is a Key Marker of Remodeling upon Stretch and Tachycardia in Human Myocardium |
| title_short | miR-1183 Is a Key Marker of Remodeling upon Stretch and Tachycardia in Human Myocardium |
| title_sort | mir 1183 is a key marker of remodeling upon stretch and tachycardia in human myocardium |
| topic | stretch tachycardia cardiac remodeling microRNA <i>MIR1183</i> miR-1183 |
| url | https://www.mdpi.com/1422-0067/23/13/6962 |
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