Programming Effect of the Parental Obesity on the Skeletal System of Offspring at Weaning Day
Our study aimed to verify the hypothesis of the existence of a programming effect of parental obesity on the growth, development and mineralization of the skeletal system in female and male rat offspring on the day of weaning. The study began with the induction of obesity in female and male rats of...
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MDPI AG
2021-02-01
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| author | Radoslaw Piotr Radzki Marek Bienko Dariusz Wolski Monika Ostapiuk Pawel Polak Malgorzata Manastyrska Aleksandra Kimicka Joanna Wolska |
| author_facet | Radoslaw Piotr Radzki Marek Bienko Dariusz Wolski Monika Ostapiuk Pawel Polak Malgorzata Manastyrska Aleksandra Kimicka Joanna Wolska |
| author_sort | Radoslaw Piotr Radzki |
| collection | DOAJ |
| description | Our study aimed to verify the hypothesis of the existence of a programming effect of parental obesity on the growth, development and mineralization of the skeletal system in female and male rat offspring on the day of weaning. The study began with the induction of obesity in female and male rats of the parental generation, using a high-energy diet (group F). Females and males of the control group received the standard diet (group S). After 90 days of dietary-induced obesity, the diet in group F was changed into the standard. Rats from groups F and S were mated to obtain offspring which stayed with their mothers until 21 days of age. Tibia was tested using dual-energy X-ray absorptiometry (DXA), peripheral quantitative computed tomography (pQCT), micro-computed tomography (µCT) and mechanical strength using the three-point bending test. Biochemical analysis of blood serum bone metabolism markers was performed. DXA analysis showed higher tibia bone mineral content (BMC) and area. pQCT measurements of cortical and trabecular tissue documented the increase of the volumetric bone mineral density and BMC of both bone compartments in offspring from the F group, while µCT of the trabecular tissue showed an increase in trabecular thickness and a decrease of its separation. Parental obesity, hence, exerts a programming influence on the development of the skeletal system of the offspring on the day of the weaning, which was reflected in the intensification of mineralization and increased bone strength. |
| first_indexed | 2024-03-09T05:19:13Z |
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| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T05:19:13Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
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| series | Animals |
| spelling | doaj.art-792d2524d8a54828ae51f4cf5eb283ce2023-12-03T12:42:18ZengMDPI AGAnimals2076-26152021-02-0111242410.3390/ani11020424Programming Effect of the Parental Obesity on the Skeletal System of Offspring at Weaning DayRadoslaw Piotr Radzki0Marek Bienko1Dariusz Wolski2Monika Ostapiuk3Pawel Polak4Malgorzata Manastyrska5Aleksandra Kimicka6Joanna Wolska7Department of Animal Physiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-033 Lublin, PolandDepartment of Animal Physiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-033 Lublin, PolandDepartment of Animal Physiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-033 Lublin, PolandDepartment of Materials Engineering, Lublin University of Technology, Nadbystrzycka 36, 20-618 Lublin, PolandSt Johns’ Oncology Center in Lublin (COZL) Trauma, Orthopaedic Surgery Department, ul. Jaczewskiego 7, 20-090 Lublin, PolandDepartment of Animal Physiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-033 Lublin, PolandDepartment of Animal Physiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-033 Lublin, PolandDepartment of Oncology, Chair of Oncology and Environmental Health, Faculty of Health Sciences, Medical University of Lublin, 20-090 Lublin, PolandOur study aimed to verify the hypothesis of the existence of a programming effect of parental obesity on the growth, development and mineralization of the skeletal system in female and male rat offspring on the day of weaning. The study began with the induction of obesity in female and male rats of the parental generation, using a high-energy diet (group F). Females and males of the control group received the standard diet (group S). After 90 days of dietary-induced obesity, the diet in group F was changed into the standard. Rats from groups F and S were mated to obtain offspring which stayed with their mothers until 21 days of age. Tibia was tested using dual-energy X-ray absorptiometry (DXA), peripheral quantitative computed tomography (pQCT), micro-computed tomography (µCT) and mechanical strength using the three-point bending test. Biochemical analysis of blood serum bone metabolism markers was performed. DXA analysis showed higher tibia bone mineral content (BMC) and area. pQCT measurements of cortical and trabecular tissue documented the increase of the volumetric bone mineral density and BMC of both bone compartments in offspring from the F group, while µCT of the trabecular tissue showed an increase in trabecular thickness and a decrease of its separation. Parental obesity, hence, exerts a programming influence on the development of the skeletal system of the offspring on the day of the weaning, which was reflected in the intensification of mineralization and increased bone strength.https://www.mdpi.com/2076-2615/11/2/424prenatal programmingnutritionbone metabolism and developmentmetabolic diseasesosteoporosisbone mechanical examination |
| spellingShingle | Radoslaw Piotr Radzki Marek Bienko Dariusz Wolski Monika Ostapiuk Pawel Polak Malgorzata Manastyrska Aleksandra Kimicka Joanna Wolska Programming Effect of the Parental Obesity on the Skeletal System of Offspring at Weaning Day Animals prenatal programming nutrition bone metabolism and development metabolic diseases osteoporosis bone mechanical examination |
| title | Programming Effect of the Parental Obesity on the Skeletal System of Offspring at Weaning Day |
| title_full | Programming Effect of the Parental Obesity on the Skeletal System of Offspring at Weaning Day |
| title_fullStr | Programming Effect of the Parental Obesity on the Skeletal System of Offspring at Weaning Day |
| title_full_unstemmed | Programming Effect of the Parental Obesity on the Skeletal System of Offspring at Weaning Day |
| title_short | Programming Effect of the Parental Obesity on the Skeletal System of Offspring at Weaning Day |
| title_sort | programming effect of the parental obesity on the skeletal system of offspring at weaning day |
| topic | prenatal programming nutrition bone metabolism and development metabolic diseases osteoporosis bone mechanical examination |
| url | https://www.mdpi.com/2076-2615/11/2/424 |
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