Influence of cytokinins and medium texture on organogenesis and plantlet regeneration in date palm (Phoenix dactylifera L.) cv. Najda
Najda cv. is an important date palm cultivar due to its high fruit quality and resistance to bayoud, a disease that killed million plants of date palm. The in vitro propagation of cv. Najda through organogenesis is hampered by some physiological disorders, namely hyperhydricity, tissue browning and...
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| Format: | Article |
| Language: | English |
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National Institute of Agronomic Research "INRA" Morocco
2022-03-01
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| Series: | African and Mediterranean Agricultural Journal - Al Awamia |
| Subjects: | |
| Online Access: | https://revues.imist.ma/index.php/Afrimed/article/view/31378 |
| _version_ | 1797793659005960192 |
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| author | M. A. Mazri R. Meziani M. Anjarne S. Elmaataoui |
| author_facet | M. A. Mazri R. Meziani M. Anjarne S. Elmaataoui |
| author_sort | M. A. Mazri |
| collection | DOAJ |
| description | Najda cv. is an important date palm cultivar due to its high fruit quality and resistance to bayoud, a disease that killed million plants of date palm. The in vitro propagation of cv. Najda through organogenesis is hampered by some physiological disorders, namely hyperhydricity, tissue browning and precocious rooting. In order to achieve efficient and large-scale propagation of true-to-type plantlets of cv. Najda, we evaluated the effect of different cytokinin types and concentrations (6-benzylaminopurine (BAP), 6-(dimethylallylamino) purine (2iP) and thidiazuron (TDZ), at 0.1, 0.2, 0.3, 0.4 or 0.5 mg/L), as well as that of medium texture (semi-solid and liquid) on adventitious shoot bud multiplication, hyperhydricity, tissue browning and precocious rooting. The optimal culture conditions for shoot bud proliferation and subsequent development were observed on semi-solid half-strength Murashige and Skoog (MS/2) medium supplemented with 0.5 mg/L 1-naphthaleneacetic acid (NAA) and 0.4 mg/L BAP, where an average of 23.8 shoot buds per explant, low rate of hyperhydricity (22.5%), moderate tissue browning (55.0%) and low percentage of precocious rooting (20.0%) were observed. Shoot elongation and rooting were performed on semi-solid and liquid plant growth regulator (PGR)-free MS/2 medium. Shoots were then transferred to the glasshouse, where the survival rate reached 95 %. The findings of the present study will be highly beneficial to rehabilitate the Moroccan palm groves infested by bayoud. |
| first_indexed | 2024-03-13T02:51:31Z |
| format | Article |
| id | doaj.art-79311ad6ca12429a9a7730d4b34ef405 |
| institution | Directory Open Access Journal |
| issn | 2658-9184 |
| language | English |
| last_indexed | 2024-03-13T02:51:31Z |
| publishDate | 2022-03-01 |
| publisher | National Institute of Agronomic Research "INRA" Morocco |
| record_format | Article |
| series | African and Mediterranean Agricultural Journal - Al Awamia |
| spelling | doaj.art-79311ad6ca12429a9a7730d4b34ef4052023-06-28T11:12:54ZengNational Institute of Agronomic Research "INRA" MoroccoAfrican and Mediterranean Agricultural Journal - Al Awamia2658-91842022-03-010130345310.34874/IMIST.PRSM/afrimed-i130.3137814987Influence of cytokinins and medium texture on organogenesis and plantlet regeneration in date palm (Phoenix dactylifera L.) cv. NajdaM. A. Mazri0R. Meziani1M. Anjarne2S. Elmaataoui3INRA / Centre Régional de la Recherche Agronomique de MarrakechINRA / Centre Régional de la Recherche Agronomique d'ErrachidiaINRA / Centre Régional de la Recherche Agronomique de MarrakechINRA / Centre Régional de la Recherche Agronomique de MarrakechNajda cv. is an important date palm cultivar due to its high fruit quality and resistance to bayoud, a disease that killed million plants of date palm. The in vitro propagation of cv. Najda through organogenesis is hampered by some physiological disorders, namely hyperhydricity, tissue browning and precocious rooting. In order to achieve efficient and large-scale propagation of true-to-type plantlets of cv. Najda, we evaluated the effect of different cytokinin types and concentrations (6-benzylaminopurine (BAP), 6-(dimethylallylamino) purine (2iP) and thidiazuron (TDZ), at 0.1, 0.2, 0.3, 0.4 or 0.5 mg/L), as well as that of medium texture (semi-solid and liquid) on adventitious shoot bud multiplication, hyperhydricity, tissue browning and precocious rooting. The optimal culture conditions for shoot bud proliferation and subsequent development were observed on semi-solid half-strength Murashige and Skoog (MS/2) medium supplemented with 0.5 mg/L 1-naphthaleneacetic acid (NAA) and 0.4 mg/L BAP, where an average of 23.8 shoot buds per explant, low rate of hyperhydricity (22.5%), moderate tissue browning (55.0%) and low percentage of precocious rooting (20.0%) were observed. Shoot elongation and rooting were performed on semi-solid and liquid plant growth regulator (PGR)-free MS/2 medium. Shoots were then transferred to the glasshouse, where the survival rate reached 95 %. The findings of the present study will be highly beneficial to rehabilitate the Moroccan palm groves infested by bayoud.https://revues.imist.ma/index.php/Afrimed/article/view/31378brunissement des tissuscytokinineenracinement précoceorganogenèsepalmier dattiervitrification |
| spellingShingle | M. A. Mazri R. Meziani M. Anjarne S. Elmaataoui Influence of cytokinins and medium texture on organogenesis and plantlet regeneration in date palm (Phoenix dactylifera L.) cv. Najda African and Mediterranean Agricultural Journal - Al Awamia brunissement des tissus cytokinine enracinement précoce organogenèse palmier dattier vitrification |
| title | Influence of cytokinins and medium texture on organogenesis and plantlet regeneration in date palm (Phoenix dactylifera L.) cv. Najda |
| title_full | Influence of cytokinins and medium texture on organogenesis and plantlet regeneration in date palm (Phoenix dactylifera L.) cv. Najda |
| title_fullStr | Influence of cytokinins and medium texture on organogenesis and plantlet regeneration in date palm (Phoenix dactylifera L.) cv. Najda |
| title_full_unstemmed | Influence of cytokinins and medium texture on organogenesis and plantlet regeneration in date palm (Phoenix dactylifera L.) cv. Najda |
| title_short | Influence of cytokinins and medium texture on organogenesis and plantlet regeneration in date palm (Phoenix dactylifera L.) cv. Najda |
| title_sort | influence of cytokinins and medium texture on organogenesis and plantlet regeneration in date palm phoenix dactylifera l cv najda |
| topic | brunissement des tissus cytokinine enracinement précoce organogenèse palmier dattier vitrification |
| url | https://revues.imist.ma/index.php/Afrimed/article/view/31378 |
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