Progesterone Has No Impact on the Beneficial Effects of Estradiol Treatment in High-Fat-Fed Ovariectomized Mice
In recent decades, clinical and experimental studies have revealed that estradiol contributes enormously to glycemic homeostasis. However, the same consensus does not exist in women during menopause who undergo replacement with progesterone or conjugated estradiol and progesterone. Since most hormon...
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MDPI AG
2023-05-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/45/5/253 |
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| author | Carlos H. Z. Talarico Ester S. Alves Jessica D. M. Dos Santos Felipe G. S. Sucupira Layanne C. C. Araujo João Paulo Camporez |
| author_facet | Carlos H. Z. Talarico Ester S. Alves Jessica D. M. Dos Santos Felipe G. S. Sucupira Layanne C. C. Araujo João Paulo Camporez |
| author_sort | Carlos H. Z. Talarico |
| collection | DOAJ |
| description | In recent decades, clinical and experimental studies have revealed that estradiol contributes enormously to glycemic homeostasis. However, the same consensus does not exist in women during menopause who undergo replacement with progesterone or conjugated estradiol and progesterone. Since most hormone replacement treatments in menopausal women are performed with estradiol (E2) and progesterone (P4) combined, this work aimed to investigate the effects of progesterone on energy metabolism and insulin resistance in an experimental model of menopause (ovariectomized female mice—OVX mice) fed a high-fat diet (HFD). OVX mice were treated with E2 or P4 (or both combined). OVX mice treated with E2 alone or combined with P4 displayed reduced body weight after six weeks of HFD feeding compared to OVX mice and OVX mice treated with P4 alone. These data were associated with improved glucose tolerance and insulin sensitivity in OVX mice treated with E2 (alone or combined with P4) compared to OVX and P4-treated mice. Additionally, E2 treatment (alone or combined with P4) reduced both hepatic and muscle triglyceride content compared with OVX control mice and OVX + P4 mice. There were no differences between groups regarding hepatic enzymes in plasma and inflammatory markers. Therefore, our results revealed that progesterone replacement alone does not seem to influence glucose homeostasis and ectopic lipid accumulation in OVX mice. These results will help expand knowledge about hormone replacement in postmenopausal women associated with metabolic syndrome and non-alcoholic fatty liver disease. |
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| last_indexed | 2024-03-11T03:50:49Z |
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| series | Current Issues in Molecular Biology |
| spelling | doaj.art-7931779d4d1545e6a5b9b28132e7f2822023-11-18T00:56:54ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452023-05-014553965397610.3390/cimb45050253Progesterone Has No Impact on the Beneficial Effects of Estradiol Treatment in High-Fat-Fed Ovariectomized MiceCarlos H. Z. Talarico0Ester S. Alves1Jessica D. M. Dos Santos2Felipe G. S. Sucupira3Layanne C. C. Araujo4João Paulo Camporez5Department of Physiology, Ribeirao Preto School of Medicine, University of Sao Paulo, Ribeirão Preto 14049-900, BrazilDepartment of Physiology, Ribeirao Preto School of Medicine, University of Sao Paulo, Ribeirão Preto 14049-900, BrazilDepartment of Physiology, Ribeirao Preto School of Medicine, University of Sao Paulo, Ribeirão Preto 14049-900, BrazilDepartment of Physiology, Ribeirao Preto School of Medicine, University of Sao Paulo, Ribeirão Preto 14049-900, BrazilDepartment of Physiology, Ribeirao Preto School of Medicine, University of Sao Paulo, Ribeirão Preto 14049-900, BrazilDepartment of Physiology, Ribeirao Preto School of Medicine, University of Sao Paulo, Ribeirão Preto 14049-900, BrazilIn recent decades, clinical and experimental studies have revealed that estradiol contributes enormously to glycemic homeostasis. However, the same consensus does not exist in women during menopause who undergo replacement with progesterone or conjugated estradiol and progesterone. Since most hormone replacement treatments in menopausal women are performed with estradiol (E2) and progesterone (P4) combined, this work aimed to investigate the effects of progesterone on energy metabolism and insulin resistance in an experimental model of menopause (ovariectomized female mice—OVX mice) fed a high-fat diet (HFD). OVX mice were treated with E2 or P4 (or both combined). OVX mice treated with E2 alone or combined with P4 displayed reduced body weight after six weeks of HFD feeding compared to OVX mice and OVX mice treated with P4 alone. These data were associated with improved glucose tolerance and insulin sensitivity in OVX mice treated with E2 (alone or combined with P4) compared to OVX and P4-treated mice. Additionally, E2 treatment (alone or combined with P4) reduced both hepatic and muscle triglyceride content compared with OVX control mice and OVX + P4 mice. There were no differences between groups regarding hepatic enzymes in plasma and inflammatory markers. Therefore, our results revealed that progesterone replacement alone does not seem to influence glucose homeostasis and ectopic lipid accumulation in OVX mice. These results will help expand knowledge about hormone replacement in postmenopausal women associated with metabolic syndrome and non-alcoholic fatty liver disease.https://www.mdpi.com/1467-3045/45/5/253menopauseovariectomy glucose metabolismmetabolic syndrome |
| spellingShingle | Carlos H. Z. Talarico Ester S. Alves Jessica D. M. Dos Santos Felipe G. S. Sucupira Layanne C. C. Araujo João Paulo Camporez Progesterone Has No Impact on the Beneficial Effects of Estradiol Treatment in High-Fat-Fed Ovariectomized Mice Current Issues in Molecular Biology menopause ovariectomy glucose metabolism metabolic syndrome |
| title | Progesterone Has No Impact on the Beneficial Effects of Estradiol Treatment in High-Fat-Fed Ovariectomized Mice |
| title_full | Progesterone Has No Impact on the Beneficial Effects of Estradiol Treatment in High-Fat-Fed Ovariectomized Mice |
| title_fullStr | Progesterone Has No Impact on the Beneficial Effects of Estradiol Treatment in High-Fat-Fed Ovariectomized Mice |
| title_full_unstemmed | Progesterone Has No Impact on the Beneficial Effects of Estradiol Treatment in High-Fat-Fed Ovariectomized Mice |
| title_short | Progesterone Has No Impact on the Beneficial Effects of Estradiol Treatment in High-Fat-Fed Ovariectomized Mice |
| title_sort | progesterone has no impact on the beneficial effects of estradiol treatment in high fat fed ovariectomized mice |
| topic | menopause ovariectomy glucose metabolism metabolic syndrome |
| url | https://www.mdpi.com/1467-3045/45/5/253 |
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