Zearalenone Removal from Corn Oil by an Enzymatic Strategy
The estrogen-like mycotoxin zearalenone (ZEN) is one of the most widely distributed contaminants especially in maize and its commodities, such as corn oil. ZEN degrading enzymes possess the potential for counteracting the negative effect of ZEN and its associated high safety risk in corn oil. Herein...
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MDPI AG
2020-02-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/12/2/117 |
| _version_ | 1798040330776346624 |
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| author | Xiaojiao Chang Hujun Liu Jing Sun Jun Wang Chengcheng Zhao Wan Zhang Jie Zhang Changpo Sun |
| author_facet | Xiaojiao Chang Hujun Liu Jing Sun Jun Wang Chengcheng Zhao Wan Zhang Jie Zhang Changpo Sun |
| author_sort | Xiaojiao Chang |
| collection | DOAJ |
| description | The estrogen-like mycotoxin zearalenone (ZEN) is one of the most widely distributed contaminants especially in maize and its commodities, such as corn oil. ZEN degrading enzymes possess the potential for counteracting the negative effect of ZEN and its associated high safety risk in corn oil. Herein, we targeted enhancing the secretion of ZEN degrading enzyme by <i>Pichia pastoris</i> through constructing an expression plasmid containing three optimized expression cassettes of <i>zlhy-6</i> codon and signal peptides. Further, we explored various parameters of enzymatic detoxification in neutralized oil and analyzed tocopherols and sterols losses in the corn oil. In addition, the distribution of degraded products was demonstrated as well by Agilent 6510 Quadrupole Time-of-Flight mass spectrometry. <i>P. pastoris</i> GSZ with the glucoamylase signal was observed with the highest ZLHY-6 secretion yield of 0.39 mg/mL. During the refining of corn oil, ZEN in the crude oil was reduced from 1257.3 to 13 µg/kg (3.69% residual) after neutralization and enzymatic detoxification. Compared with the neutralized oil, no significant difference in the total tocopherols and sterols contents was detected after enzymatic detoxification. Finally, the degraded products were found to be entirely eliminated by washing. This study presents an enzymatic strategy for efficient and safe ZEN removal with relatively low nutrient loss, which provides an important basis for further application of enzymatic ZEN elimination in the industrial process of corn oil production. |
| first_indexed | 2024-04-11T22:06:07Z |
| format | Article |
| id | doaj.art-7932dc3eee1740eb91286633d70a7367 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T22:06:07Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-7932dc3eee1740eb91286633d70a73672022-12-22T04:00:42ZengMDPI AGToxins2072-66512020-02-0112211710.3390/toxins12020117toxins12020117Zearalenone Removal from Corn Oil by an Enzymatic StrategyXiaojiao Chang0Hujun Liu1Jing Sun2Jun Wang3Chengcheng Zhao4Wan Zhang5Jie Zhang6Changpo Sun7Institute of Grain & Oil Science and Technology, Academy of National Food and Strategic Reserves Administration, Beijing 100037, ChinaInstitute of Grain & Oil Science and Technology, Academy of National Food and Strategic Reserves Administration, Beijing 100037, ChinaInstitute of Grain & Oil Science and Technology, Academy of National Food and Strategic Reserves Administration, Beijing 100037, ChinaInstitute of Grain & Oil Science and Technology, Academy of National Food and Strategic Reserves Administration, Beijing 100037, ChinaInstitute of Grain & Oil Science and Technology, Academy of National Food and Strategic Reserves Administration, Beijing 100037, ChinaInstitute of Grain & Oil Science and Technology, Academy of National Food and Strategic Reserves Administration, Beijing 100037, ChinaInstitute of Plant Protection, Chinese Academy of Agricultural Science, Beijing 100094, ChinaInstitute of Grain & Oil Science and Technology, Academy of National Food and Strategic Reserves Administration, Beijing 100037, ChinaThe estrogen-like mycotoxin zearalenone (ZEN) is one of the most widely distributed contaminants especially in maize and its commodities, such as corn oil. ZEN degrading enzymes possess the potential for counteracting the negative effect of ZEN and its associated high safety risk in corn oil. Herein, we targeted enhancing the secretion of ZEN degrading enzyme by <i>Pichia pastoris</i> through constructing an expression plasmid containing three optimized expression cassettes of <i>zlhy-6</i> codon and signal peptides. Further, we explored various parameters of enzymatic detoxification in neutralized oil and analyzed tocopherols and sterols losses in the corn oil. In addition, the distribution of degraded products was demonstrated as well by Agilent 6510 Quadrupole Time-of-Flight mass spectrometry. <i>P. pastoris</i> GSZ with the glucoamylase signal was observed with the highest ZLHY-6 secretion yield of 0.39 mg/mL. During the refining of corn oil, ZEN in the crude oil was reduced from 1257.3 to 13 µg/kg (3.69% residual) after neutralization and enzymatic detoxification. Compared with the neutralized oil, no significant difference in the total tocopherols and sterols contents was detected after enzymatic detoxification. Finally, the degraded products were found to be entirely eliminated by washing. This study presents an enzymatic strategy for efficient and safe ZEN removal with relatively low nutrient loss, which provides an important basis for further application of enzymatic ZEN elimination in the industrial process of corn oil production.https://www.mdpi.com/2072-6651/12/2/117zearalenoneenzymatic reactiondetoxificationcorn oilrefining |
| spellingShingle | Xiaojiao Chang Hujun Liu Jing Sun Jun Wang Chengcheng Zhao Wan Zhang Jie Zhang Changpo Sun Zearalenone Removal from Corn Oil by an Enzymatic Strategy Toxins zearalenone enzymatic reaction detoxification corn oil refining |
| title | Zearalenone Removal from Corn Oil by an Enzymatic Strategy |
| title_full | Zearalenone Removal from Corn Oil by an Enzymatic Strategy |
| title_fullStr | Zearalenone Removal from Corn Oil by an Enzymatic Strategy |
| title_full_unstemmed | Zearalenone Removal from Corn Oil by an Enzymatic Strategy |
| title_short | Zearalenone Removal from Corn Oil by an Enzymatic Strategy |
| title_sort | zearalenone removal from corn oil by an enzymatic strategy |
| topic | zearalenone enzymatic reaction detoxification corn oil refining |
| url | https://www.mdpi.com/2072-6651/12/2/117 |
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