Improved Itaconate Production with <i>Ustilago cynodontis</i> via Co-Metabolism of CO<sub>2</sub>-Derived Formate
In recent years, it was shown that itaconic acid can be produced from glucose with <i>Ustilago</i> strains at up to maximum theoretical yield. The use of acetate and formate as co-feedstocks can boost the efficiency of itaconate production with Ustilaginaceae wild-type strains by reducin...
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MDPI AG
2022-12-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/12/1277 |
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| author | Lena Ullmann Nils Guntermann Philipp Kohl Gereon Schröders Andreas Müsgens Giancarlo Franciò Walter Leitner Lars M. Blank |
| author_facet | Lena Ullmann Nils Guntermann Philipp Kohl Gereon Schröders Andreas Müsgens Giancarlo Franciò Walter Leitner Lars M. Blank |
| author_sort | Lena Ullmann |
| collection | DOAJ |
| description | In recent years, it was shown that itaconic acid can be produced from glucose with <i>Ustilago</i> strains at up to maximum theoretical yield. The use of acetate and formate as co-feedstocks can boost the efficiency of itaconate production with Ustilaginaceae wild-type strains by reducing the glucose amount and thus the agricultural land required for the biotechnological production of this chemical. Metabolically engineered strains (<i>U. cynodontis</i> Δ<i>fuz7</i> Δ<i>cyp3</i> ↑P<sub>ria1</sub> and <i>U. cynodontis</i> Δ<i>fuz7</i> Δ<i>cyp3</i> P<sub>etef</sub><i>mttA</i> ↑P<sub>ria1</sub>) were applied in itaconate production, obtaining a titer of 56.1 g L<sup>−1</sup> and a yield of 0.55 g<sub>itaconate</sub> per g<sub>substrate</sub>. Both improved titer and yield (increase of 5.2 g L<sup>−1</sup> and 0.04 g<sub>itaconate</sub> per g<sub>substrate</sub>, respectively) were achieved when using sodium formate as an auxiliary substrate. By applying the design-of-experiments (DoE) methodology, cultivation parameters (glucose, sodium formate and ammonium chloride concentrations) were optimized, resulting in two empirical models predicting itaconate titer and yield for <i>U. cynodontis</i> Δ<i>fuz7</i> Δ<i>cyp3</i> P<sub>etef</sub><i>mttA</i> ↑P<sub>ria1</sub>. Thereby, an almost doubled itaconate titer of 138 g L<sup>−1</sup> was obtained and a yield of 0.62 g<sub>itaconate</sub> per g<sub>substrate</sub> was reached during confirmation experiments corresponding to 86% of the theoretical maximum. In order to close the carbon cycle by production of the co-feed via a “power-to-X” route, the biphasic Ru-catalysed hydrogenation of CO<sub>2</sub> to formate could be integrated into the bioprocess directly using the obtained aqueous solution of formates as co-feedstock without any purification steps, demonstrating the (bio)compatibility of the two processes. |
| first_indexed | 2024-03-09T16:13:57Z |
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| language | English |
| last_indexed | 2024-03-09T16:13:57Z |
| publishDate | 2022-12-01 |
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| spelling | doaj.art-7938c070dd684555a25632280b8852712023-11-24T15:59:44ZengMDPI AGJournal of Fungi2309-608X2022-12-01812127710.3390/jof8121277Improved Itaconate Production with <i>Ustilago cynodontis</i> via Co-Metabolism of CO<sub>2</sub>-Derived FormateLena Ullmann0Nils Guntermann1Philipp Kohl2Gereon Schröders3Andreas Müsgens4Giancarlo Franciò5Walter Leitner6Lars M. Blank7iAMB—Institute of Applied Microbiology, ABBt—Aachen Biology and Biotechnology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, GermanyITMC—Institute for Technical and Macromolecular Chemistry, RWTH Aachen University, Worringerweg 2, 52074 Aachen, GermanyiAMB—Institute of Applied Microbiology, ABBt—Aachen Biology and Biotechnology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, GermanyiAMB—Institute of Applied Microbiology, ABBt—Aachen Biology and Biotechnology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, GermanyiAMB—Institute of Applied Microbiology, ABBt—Aachen Biology and Biotechnology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, GermanyITMC—Institute for Technical and Macromolecular Chemistry, RWTH Aachen University, Worringerweg 2, 52074 Aachen, GermanyITMC—Institute for Technical and Macromolecular Chemistry, RWTH Aachen University, Worringerweg 2, 52074 Aachen, GermanyiAMB—Institute of Applied Microbiology, ABBt—Aachen Biology and Biotechnology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, GermanyIn recent years, it was shown that itaconic acid can be produced from glucose with <i>Ustilago</i> strains at up to maximum theoretical yield. The use of acetate and formate as co-feedstocks can boost the efficiency of itaconate production with Ustilaginaceae wild-type strains by reducing the glucose amount and thus the agricultural land required for the biotechnological production of this chemical. Metabolically engineered strains (<i>U. cynodontis</i> Δ<i>fuz7</i> Δ<i>cyp3</i> ↑P<sub>ria1</sub> and <i>U. cynodontis</i> Δ<i>fuz7</i> Δ<i>cyp3</i> P<sub>etef</sub><i>mttA</i> ↑P<sub>ria1</sub>) were applied in itaconate production, obtaining a titer of 56.1 g L<sup>−1</sup> and a yield of 0.55 g<sub>itaconate</sub> per g<sub>substrate</sub>. Both improved titer and yield (increase of 5.2 g L<sup>−1</sup> and 0.04 g<sub>itaconate</sub> per g<sub>substrate</sub>, respectively) were achieved when using sodium formate as an auxiliary substrate. By applying the design-of-experiments (DoE) methodology, cultivation parameters (glucose, sodium formate and ammonium chloride concentrations) were optimized, resulting in two empirical models predicting itaconate titer and yield for <i>U. cynodontis</i> Δ<i>fuz7</i> Δ<i>cyp3</i> P<sub>etef</sub><i>mttA</i> ↑P<sub>ria1</sub>. Thereby, an almost doubled itaconate titer of 138 g L<sup>−1</sup> was obtained and a yield of 0.62 g<sub>itaconate</sub> per g<sub>substrate</sub> was reached during confirmation experiments corresponding to 86% of the theoretical maximum. In order to close the carbon cycle by production of the co-feed via a “power-to-X” route, the biphasic Ru-catalysed hydrogenation of CO<sub>2</sub> to formate could be integrated into the bioprocess directly using the obtained aqueous solution of formates as co-feedstock without any purification steps, demonstrating the (bio)compatibility of the two processes.https://www.mdpi.com/2309-608X/8/12/1277itaconateitaconic acidCO<sub>2</sub> hydrogenationsecondary metabolitesUstilaginaceae<i>Ustilago cynodontis</i> |
| spellingShingle | Lena Ullmann Nils Guntermann Philipp Kohl Gereon Schröders Andreas Müsgens Giancarlo Franciò Walter Leitner Lars M. Blank Improved Itaconate Production with <i>Ustilago cynodontis</i> via Co-Metabolism of CO<sub>2</sub>-Derived Formate Journal of Fungi itaconate itaconic acid CO<sub>2</sub> hydrogenation secondary metabolites Ustilaginaceae <i>Ustilago cynodontis</i> |
| title | Improved Itaconate Production with <i>Ustilago cynodontis</i> via Co-Metabolism of CO<sub>2</sub>-Derived Formate |
| title_full | Improved Itaconate Production with <i>Ustilago cynodontis</i> via Co-Metabolism of CO<sub>2</sub>-Derived Formate |
| title_fullStr | Improved Itaconate Production with <i>Ustilago cynodontis</i> via Co-Metabolism of CO<sub>2</sub>-Derived Formate |
| title_full_unstemmed | Improved Itaconate Production with <i>Ustilago cynodontis</i> via Co-Metabolism of CO<sub>2</sub>-Derived Formate |
| title_short | Improved Itaconate Production with <i>Ustilago cynodontis</i> via Co-Metabolism of CO<sub>2</sub>-Derived Formate |
| title_sort | improved itaconate production with i ustilago cynodontis i via co metabolism of co sub 2 sub derived formate |
| topic | itaconate itaconic acid CO<sub>2</sub> hydrogenation secondary metabolites Ustilaginaceae <i>Ustilago cynodontis</i> |
| url | https://www.mdpi.com/2309-608X/8/12/1277 |
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