Proteomic Analysis of Female Synovial Fluid to Identify Novel Biomarkers for Osteoarthritis
Osteoarthritis (OA) is a highly prevalent degenerative joint condition that disproportionately affects females. The pathophysiology of the disease is not well understood, which makes diagnosis and treatment difficult. Given the physical connection of synovial fluid (SF) with articular tissues, the S...
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MDPI AG
2023-02-01
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| author | P. Robinson Muller Tae Jin Lee Wenbo Zhi Sandeep Kumar Sagar Vyavahare Ashok Sharma Vikas Kumar Carlos M. Isales Monte Hunter Sadanand Fulzele |
| author_facet | P. Robinson Muller Tae Jin Lee Wenbo Zhi Sandeep Kumar Sagar Vyavahare Ashok Sharma Vikas Kumar Carlos M. Isales Monte Hunter Sadanand Fulzele |
| author_sort | P. Robinson Muller |
| collection | DOAJ |
| description | Osteoarthritis (OA) is a highly prevalent degenerative joint condition that disproportionately affects females. The pathophysiology of the disease is not well understood, which makes diagnosis and treatment difficult. Given the physical connection of synovial fluid (SF) with articular tissues, the SF’s composition can reflect relevant biological modifications, and has therefore been a focus of research. Previously, we demonstrated that extracellular vesicles isolated from the synovial fluid of OA patients carry different cargo (protein and miRNA) in a sex-specific manner. Given the increased prevalence and severity of OA in females, this study aims to identify differential protein content within the synovial fluid of female OA and non-osteoarthritic (non-OA) patients. We found that several proteins were differentially expressed in osteoarthritic females compared with age-matched controls. Presenilin, Coagulation Factor X, Lysine-Specific Demethylase 2B, Tenascin C, Leucine-Rich Repeat-Containing Protein 17 fragments, and T-Complex Protein 1 were negatively regulated in the OA group, with PGD Synthase, Tubulointerstitial Nephritis Antigen, and Nuclear Receptor Binding SET Domain Protein 1 positively regulated in the OA group. Database for Annotation, Visualization, and Integrated Discovery (DAVID) and QuickGO analyses established these proteins as significantly involved in many biological, cellular, and molecular processes. In conclusion, the protein content of female synovial fluid is altered in OA patients, which is likely to provide insights into gender-specific pathophysiology. |
| first_indexed | 2024-03-11T06:17:18Z |
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| id | doaj.art-7939c623b1f94f4da525ed2b0a927e48 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-11T06:17:18Z |
| publishDate | 2023-02-01 |
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| record_format | Article |
| series | Life |
| spelling | doaj.art-7939c623b1f94f4da525ed2b0a927e482023-11-17T12:09:50ZengMDPI AGLife2075-17292023-02-0113360510.3390/life13030605Proteomic Analysis of Female Synovial Fluid to Identify Novel Biomarkers for OsteoarthritisP. Robinson Muller0Tae Jin Lee1Wenbo Zhi2Sandeep Kumar3Sagar Vyavahare4Ashok Sharma5Vikas Kumar6Carlos M. Isales7Monte Hunter8Sadanand Fulzele9Department of Medicine, Augusta University, Augusta, GA 30912, USACenter for Biotechnology and Genomic Medicine, Augusta University, Augusta, GA 30912, USACenter for Biotechnology and Genomic Medicine, Augusta University, Augusta, GA 30912, USADepartment of Medicine, Augusta University, Augusta, GA 30912, USADepartment of Medicine, Augusta University, Augusta, GA 30912, USACenter for Biotechnology and Genomic Medicine, Augusta University, Augusta, GA 30912, USAAnesthesiology & Perioperative Medicine, Augusta University, Augusta, GA 30912, USADepartment of Medicine, Augusta University, Augusta, GA 30912, USADepartment of Orthopaedic Surgery, Augusta University, Augusta, GA 30912, USADepartment of Medicine, Augusta University, Augusta, GA 30912, USAOsteoarthritis (OA) is a highly prevalent degenerative joint condition that disproportionately affects females. The pathophysiology of the disease is not well understood, which makes diagnosis and treatment difficult. Given the physical connection of synovial fluid (SF) with articular tissues, the SF’s composition can reflect relevant biological modifications, and has therefore been a focus of research. Previously, we demonstrated that extracellular vesicles isolated from the synovial fluid of OA patients carry different cargo (protein and miRNA) in a sex-specific manner. Given the increased prevalence and severity of OA in females, this study aims to identify differential protein content within the synovial fluid of female OA and non-osteoarthritic (non-OA) patients. We found that several proteins were differentially expressed in osteoarthritic females compared with age-matched controls. Presenilin, Coagulation Factor X, Lysine-Specific Demethylase 2B, Tenascin C, Leucine-Rich Repeat-Containing Protein 17 fragments, and T-Complex Protein 1 were negatively regulated in the OA group, with PGD Synthase, Tubulointerstitial Nephritis Antigen, and Nuclear Receptor Binding SET Domain Protein 1 positively regulated in the OA group. Database for Annotation, Visualization, and Integrated Discovery (DAVID) and QuickGO analyses established these proteins as significantly involved in many biological, cellular, and molecular processes. In conclusion, the protein content of female synovial fluid is altered in OA patients, which is likely to provide insights into gender-specific pathophysiology.https://www.mdpi.com/2075-1729/13/3/605proteomicfemalesynovial fluidosteoarthritis |
| spellingShingle | P. Robinson Muller Tae Jin Lee Wenbo Zhi Sandeep Kumar Sagar Vyavahare Ashok Sharma Vikas Kumar Carlos M. Isales Monte Hunter Sadanand Fulzele Proteomic Analysis of Female Synovial Fluid to Identify Novel Biomarkers for Osteoarthritis Life proteomic female synovial fluid osteoarthritis |
| title | Proteomic Analysis of Female Synovial Fluid to Identify Novel Biomarkers for Osteoarthritis |
| title_full | Proteomic Analysis of Female Synovial Fluid to Identify Novel Biomarkers for Osteoarthritis |
| title_fullStr | Proteomic Analysis of Female Synovial Fluid to Identify Novel Biomarkers for Osteoarthritis |
| title_full_unstemmed | Proteomic Analysis of Female Synovial Fluid to Identify Novel Biomarkers for Osteoarthritis |
| title_short | Proteomic Analysis of Female Synovial Fluid to Identify Novel Biomarkers for Osteoarthritis |
| title_sort | proteomic analysis of female synovial fluid to identify novel biomarkers for osteoarthritis |
| topic | proteomic female synovial fluid osteoarthritis |
| url | https://www.mdpi.com/2075-1729/13/3/605 |
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