<i>Moringa oleifera</i> Leaf Extract Promotes Healing of Infected Wounds in Diabetic Rats: Evidence of Antimicrobial, Antioxidant and Proliferative Properties
<i>Moringa oleifera</i> is known to possess wound healing activity. The present study evaluated the healing properties of methanolic extract of <i>M. oleifera</i> leaves in excision wounds infected with methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) or...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-04-01
|
| Series: | Pharmaceuticals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8247/15/5/528 |
| _version_ | 1797496944326606848 |
|---|---|
| author | Abdullah A. Al-Ghanayem Mohammed Sanad Alhussaini Mohammed Asad Babu Joseph |
| author_facet | Abdullah A. Al-Ghanayem Mohammed Sanad Alhussaini Mohammed Asad Babu Joseph |
| author_sort | Abdullah A. Al-Ghanayem |
| collection | DOAJ |
| description | <i>Moringa oleifera</i> is known to possess wound healing activity. The present study evaluated the healing properties of methanolic extract of <i>M. oleifera</i> leaves in excision wounds infected with methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) or <i>P. aeruginosa</i> in diabetic rats. An in vitro study was also carried out to determine the gene expression of VEGF and TGF-β1. Preliminary phytochemical and GC-MS analyses were carried out to determine different chemical constituents present in the extract. <i>M. oleifera</i> was applied locally as an ointment at two different concentrations. Wound contraction, period of epithelization, antioxidant enzyme activities and histological changes were determined. For the gene expression study, HaCaT cell lines were used. The formulation of <i>M. oleifera</i> extract improved wound contraction and decreased the period of epithelization, which was associated with an increase in antioxidant enzyme activities, epithelization, capillary density and collagen formation in <i>MRSA</i>-infected diabetic rats. However, this effect was reduced in diabetic animals infected with <i>P. aeruginosa</i>. An increase in the expression of VEGF and TGF-β1 was observed in HaCaT cell lines. <i>M. oleifera</i> extract promotes the healing of infected wounds in <i>MRSA</i>-infected diabetic rats but is less effective in the healing of wounds infected with <i>P. aeruginosa</i> in diabetic rats. |
| first_indexed | 2024-03-10T03:10:31Z |
| format | Article |
| id | doaj.art-793a854c3d104ad6aac8d35e237dd2bd |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-10T03:10:31Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-793a854c3d104ad6aac8d35e237dd2bd2023-11-23T12:34:00ZengMDPI AGPharmaceuticals1424-82472022-04-0115552810.3390/ph15050528<i>Moringa oleifera</i> Leaf Extract Promotes Healing of Infected Wounds in Diabetic Rats: Evidence of Antimicrobial, Antioxidant and Proliferative PropertiesAbdullah A. Al-Ghanayem0Mohammed Sanad Alhussaini1Mohammed Asad2Babu Joseph3Department of Clinical Laboratory Science, College of Applied Medical Sciences, Shaqra University, Shaqra 11961, Saudi ArabiaDepartment of Clinical Laboratory Science, College of Applied Medical Sciences, Shaqra University, Shaqra 11961, Saudi ArabiaDepartment of Clinical Laboratory Science, College of Applied Medical Sciences, Shaqra University, Shaqra 11961, Saudi ArabiaDepartment of Clinical Laboratory Science, College of Applied Medical Sciences, Shaqra University, Shaqra 11961, Saudi Arabia<i>Moringa oleifera</i> is known to possess wound healing activity. The present study evaluated the healing properties of methanolic extract of <i>M. oleifera</i> leaves in excision wounds infected with methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) or <i>P. aeruginosa</i> in diabetic rats. An in vitro study was also carried out to determine the gene expression of VEGF and TGF-β1. Preliminary phytochemical and GC-MS analyses were carried out to determine different chemical constituents present in the extract. <i>M. oleifera</i> was applied locally as an ointment at two different concentrations. Wound contraction, period of epithelization, antioxidant enzyme activities and histological changes were determined. For the gene expression study, HaCaT cell lines were used. The formulation of <i>M. oleifera</i> extract improved wound contraction and decreased the period of epithelization, which was associated with an increase in antioxidant enzyme activities, epithelization, capillary density and collagen formation in <i>MRSA</i>-infected diabetic rats. However, this effect was reduced in diabetic animals infected with <i>P. aeruginosa</i>. An increase in the expression of VEGF and TGF-β1 was observed in HaCaT cell lines. <i>M. oleifera</i> extract promotes the healing of infected wounds in <i>MRSA</i>-infected diabetic rats but is less effective in the healing of wounds infected with <i>P. aeruginosa</i> in diabetic rats.https://www.mdpi.com/1424-8247/15/5/528capillary densitycollagenepithelizationVEGFTGF-β1 |
| spellingShingle | Abdullah A. Al-Ghanayem Mohammed Sanad Alhussaini Mohammed Asad Babu Joseph <i>Moringa oleifera</i> Leaf Extract Promotes Healing of Infected Wounds in Diabetic Rats: Evidence of Antimicrobial, Antioxidant and Proliferative Properties Pharmaceuticals capillary density collagen epithelization VEGF TGF-β1 |
| title | <i>Moringa oleifera</i> Leaf Extract Promotes Healing of Infected Wounds in Diabetic Rats: Evidence of Antimicrobial, Antioxidant and Proliferative Properties |
| title_full | <i>Moringa oleifera</i> Leaf Extract Promotes Healing of Infected Wounds in Diabetic Rats: Evidence of Antimicrobial, Antioxidant and Proliferative Properties |
| title_fullStr | <i>Moringa oleifera</i> Leaf Extract Promotes Healing of Infected Wounds in Diabetic Rats: Evidence of Antimicrobial, Antioxidant and Proliferative Properties |
| title_full_unstemmed | <i>Moringa oleifera</i> Leaf Extract Promotes Healing of Infected Wounds in Diabetic Rats: Evidence of Antimicrobial, Antioxidant and Proliferative Properties |
| title_short | <i>Moringa oleifera</i> Leaf Extract Promotes Healing of Infected Wounds in Diabetic Rats: Evidence of Antimicrobial, Antioxidant and Proliferative Properties |
| title_sort | i moringa oleifera i leaf extract promotes healing of infected wounds in diabetic rats evidence of antimicrobial antioxidant and proliferative properties |
| topic | capillary density collagen epithelization VEGF TGF-β1 |
| url | https://www.mdpi.com/1424-8247/15/5/528 |
| work_keys_str_mv | AT abdullahaalghanayem imoringaoleiferaileafextractpromoteshealingofinfectedwoundsindiabeticratsevidenceofantimicrobialantioxidantandproliferativeproperties AT mohammedsanadalhussaini imoringaoleiferaileafextractpromoteshealingofinfectedwoundsindiabeticratsevidenceofantimicrobialantioxidantandproliferativeproperties AT mohammedasad imoringaoleiferaileafextractpromoteshealingofinfectedwoundsindiabeticratsevidenceofantimicrobialantioxidantandproliferativeproperties AT babujoseph imoringaoleiferaileafextractpromoteshealingofinfectedwoundsindiabeticratsevidenceofantimicrobialantioxidantandproliferativeproperties |