Acclimation of Photosynthesis to Changes in the Environment Results in Decreases of Oxidative Stress in Arabidopsis thaliana
The dynamic acclimation of photosynthesis plays an important role in increasing the fitness of a plant under variable light environments. Since acclimation is partially mediated by a glucose-6-phosphate/phosphate translocator 2 (GPT2), this study examined whether plants lacking GPT2, which consequen...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.683986/full |
| _version_ | 1818717719145480192 |
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| author | Mohd Fauzihan Karim Giles N. Johnson |
| author_facet | Mohd Fauzihan Karim Giles N. Johnson |
| author_sort | Mohd Fauzihan Karim |
| collection | DOAJ |
| description | The dynamic acclimation of photosynthesis plays an important role in increasing the fitness of a plant under variable light environments. Since acclimation is partially mediated by a glucose-6-phosphate/phosphate translocator 2 (GPT2), this study examined whether plants lacking GPT2, which consequently have defective acclimation to increases in light, are more susceptible to oxidative stress. To understand this mechanism, we used the model plant Arabidopsis thaliana [accession Wassilewskija-4 (Ws-4)] and compared it with mutants lacking GPT2. The plants were then grown at low light (LL) at 100 μmol m−2 s−1 for 7 weeks. For the acclimation experiments, a set of plants from LL was transferred to 400 μmol m−2 s−1 conditions for 7 days. Biochemical and physiological analyses showed that the gpt2 mutant plants had significantly greater activity for ascorbate peroxidase (APX), guiacol peroxidase (GPOX), and superoxide dismutase (SOD). Furthermore, the mutant plants had significantly lower maximum quantum yields of photosynthesis (Fv/Fm). A microarray analysis also showed that gpt2 plants exhibited a greater induction of stress-related genes relative to wild-type (WT) plants. We then concluded that photosynthetic acclimation to a higher intensity of light protects plants against oxidative stress. |
| first_indexed | 2024-12-17T19:39:37Z |
| format | Article |
| id | doaj.art-793ada0100cd4029bf82b99b534a176a |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-17T19:39:37Z |
| publishDate | 2021-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-793ada0100cd4029bf82b99b534a176a2022-12-21T21:35:02ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-09-011210.3389/fpls.2021.683986683986Acclimation of Photosynthesis to Changes in the Environment Results in Decreases of Oxidative Stress in Arabidopsis thalianaMohd Fauzihan KarimGiles N. JohnsonThe dynamic acclimation of photosynthesis plays an important role in increasing the fitness of a plant under variable light environments. Since acclimation is partially mediated by a glucose-6-phosphate/phosphate translocator 2 (GPT2), this study examined whether plants lacking GPT2, which consequently have defective acclimation to increases in light, are more susceptible to oxidative stress. To understand this mechanism, we used the model plant Arabidopsis thaliana [accession Wassilewskija-4 (Ws-4)] and compared it with mutants lacking GPT2. The plants were then grown at low light (LL) at 100 μmol m−2 s−1 for 7 weeks. For the acclimation experiments, a set of plants from LL was transferred to 400 μmol m−2 s−1 conditions for 7 days. Biochemical and physiological analyses showed that the gpt2 mutant plants had significantly greater activity for ascorbate peroxidase (APX), guiacol peroxidase (GPOX), and superoxide dismutase (SOD). Furthermore, the mutant plants had significantly lower maximum quantum yields of photosynthesis (Fv/Fm). A microarray analysis also showed that gpt2 plants exhibited a greater induction of stress-related genes relative to wild-type (WT) plants. We then concluded that photosynthetic acclimation to a higher intensity of light protects plants against oxidative stress.https://www.frontiersin.org/articles/10.3389/fpls.2021.683986/fulllight stressoxidative stressphotosynthetic acclimationantioxidantsreactive oxygen species |
| spellingShingle | Mohd Fauzihan Karim Giles N. Johnson Acclimation of Photosynthesis to Changes in the Environment Results in Decreases of Oxidative Stress in Arabidopsis thaliana Frontiers in Plant Science light stress oxidative stress photosynthetic acclimation antioxidants reactive oxygen species |
| title | Acclimation of Photosynthesis to Changes in the Environment Results in Decreases of Oxidative Stress in Arabidopsis thaliana |
| title_full | Acclimation of Photosynthesis to Changes in the Environment Results in Decreases of Oxidative Stress in Arabidopsis thaliana |
| title_fullStr | Acclimation of Photosynthesis to Changes in the Environment Results in Decreases of Oxidative Stress in Arabidopsis thaliana |
| title_full_unstemmed | Acclimation of Photosynthesis to Changes in the Environment Results in Decreases of Oxidative Stress in Arabidopsis thaliana |
| title_short | Acclimation of Photosynthesis to Changes in the Environment Results in Decreases of Oxidative Stress in Arabidopsis thaliana |
| title_sort | acclimation of photosynthesis to changes in the environment results in decreases of oxidative stress in arabidopsis thaliana |
| topic | light stress oxidative stress photosynthetic acclimation antioxidants reactive oxygen species |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.683986/full |
| work_keys_str_mv | AT mohdfauzihankarim acclimationofphotosynthesistochangesintheenvironmentresultsindecreasesofoxidativestressinarabidopsisthaliana AT gilesnjohnson acclimationofphotosynthesistochangesintheenvironmentresultsindecreasesofoxidativestressinarabidopsisthaliana |