Biological Impact of γ-Fe<sub>2</sub>O<sub>3</sub> Magnetic Nanoparticles Obtained by Laser Target Evaporation: Focus on Magnetic Biosensor Applications
The biological activity of γ-Fe<sub>2</sub>O<sub>3</sub> magnetic nanoparticles (MNPs), obtained by the laser target evaporation technique, was studied, with a focus on their possible use in biosensor applications. The biological effect of the MNPs was investigated in vitro o...
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MDPI AG
2022-08-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/8/627 |
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| author | Fedor A. Fadeyev Felix A. Blyakhman Alexander P. Safronov Grigory Yu. Melnikov Anastasia D. Nikanorova Iuliia P. Novoselova Galina V. Kurlyandskaya |
| author_facet | Fedor A. Fadeyev Felix A. Blyakhman Alexander P. Safronov Grigory Yu. Melnikov Anastasia D. Nikanorova Iuliia P. Novoselova Galina V. Kurlyandskaya |
| author_sort | Fedor A. Fadeyev |
| collection | DOAJ |
| description | The biological activity of γ-Fe<sub>2</sub>O<sub>3</sub> magnetic nanoparticles (MNPs), obtained by the laser target evaporation technique, was studied, with a focus on their possible use in biosensor applications. The biological effect of the MNPs was investigated in vitro on the primary cultures of human dermal fibroblasts. The effects of the MNPs contained in culture medium or MNPs already uptaken by cells were evaluated for the cases of the fibroblast’s proliferation and secretion of cytokines and collagen. For the tests related to the contribution of the constant magnetic field to the biological activity of MNPs, a magnetic system for the creation of the external magnetic field (having no commercial analogues) was designed, calibrated, and used. It was adapted to the size of standard 24-well cell culture plates. At low concentrations of MNPs, uptake by fibroblasts had stimulated their proliferation. Extracellular MNPs stimulated the release of pro-inflammatory cytokines (Interleukin-6 (IL-6) and Interleukin-8 (IL-8) or chemokine (C-X-C motif) ligand 8 (CXCL8)) in a concentration-dependent manner. However, the presence of MNPs did not increase the collagen secretion. The exposure to the uniform constant magnetic field (H ≈ 630 or 320 Oe), oriented in the plane of the well, did not cause considerable changes in fibroblasts proliferation and secretion, regardless of presence of MNPs. Statistically significant differences were detected only in the levels of IL-8/CXCL8 release. |
| first_indexed | 2024-03-09T04:39:06Z |
| format | Article |
| id | doaj.art-793d1751e21442c59d4d00ab7036faad |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T04:39:06Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-793d1751e21442c59d4d00ab7036faad2023-12-03T13:23:14ZengMDPI AGBiosensors2079-63742022-08-0112862710.3390/bios12080627Biological Impact of γ-Fe<sub>2</sub>O<sub>3</sub> Magnetic Nanoparticles Obtained by Laser Target Evaporation: Focus on Magnetic Biosensor ApplicationsFedor A. Fadeyev0Felix A. Blyakhman1Alexander P. Safronov2Grigory Yu. Melnikov3Anastasia D. Nikanorova4Iuliia P. Novoselova5Galina V. Kurlyandskaya6Department of Biomedical Physics and Engineering, Ural State Medical University, 620028 Ekaterinburg, RussiaDepartment of Biomedical Physics and Engineering, Ural State Medical University, 620028 Ekaterinburg, RussiaInstitute of Natural Sciences and Mathematics, Ural Federal University, 620002 Ekaterinburg, RussiaInstitute of Natural Sciences and Mathematics, Ural Federal University, 620002 Ekaterinburg, RussiaInstitute of Medical Cell Technologies, 620026 Ekaterinburg, RussiaInstitute of Human Genetics, Medical Faculty, University Hospital Düsseldorf, Heinrich-Heine-University Düsseldorf, 40225 Düsseldorf, GermanyInstitute of Natural Sciences and Mathematics, Ural Federal University, 620002 Ekaterinburg, RussiaThe biological activity of γ-Fe<sub>2</sub>O<sub>3</sub> magnetic nanoparticles (MNPs), obtained by the laser target evaporation technique, was studied, with a focus on their possible use in biosensor applications. The biological effect of the MNPs was investigated in vitro on the primary cultures of human dermal fibroblasts. The effects of the MNPs contained in culture medium or MNPs already uptaken by cells were evaluated for the cases of the fibroblast’s proliferation and secretion of cytokines and collagen. For the tests related to the contribution of the constant magnetic field to the biological activity of MNPs, a magnetic system for the creation of the external magnetic field (having no commercial analogues) was designed, calibrated, and used. It was adapted to the size of standard 24-well cell culture plates. At low concentrations of MNPs, uptake by fibroblasts had stimulated their proliferation. Extracellular MNPs stimulated the release of pro-inflammatory cytokines (Interleukin-6 (IL-6) and Interleukin-8 (IL-8) or chemokine (C-X-C motif) ligand 8 (CXCL8)) in a concentration-dependent manner. However, the presence of MNPs did not increase the collagen secretion. The exposure to the uniform constant magnetic field (H ≈ 630 or 320 Oe), oriented in the plane of the well, did not cause considerable changes in fibroblasts proliferation and secretion, regardless of presence of MNPs. Statistically significant differences were detected only in the levels of IL-8/CXCL8 release.https://www.mdpi.com/2079-6374/12/8/627magnetic nanoparticlesconstant magnetic fieldbiomedical applicationshuman skin fibroblastscell proliferationcytokine secretion |
| spellingShingle | Fedor A. Fadeyev Felix A. Blyakhman Alexander P. Safronov Grigory Yu. Melnikov Anastasia D. Nikanorova Iuliia P. Novoselova Galina V. Kurlyandskaya Biological Impact of γ-Fe<sub>2</sub>O<sub>3</sub> Magnetic Nanoparticles Obtained by Laser Target Evaporation: Focus on Magnetic Biosensor Applications Biosensors magnetic nanoparticles constant magnetic field biomedical applications human skin fibroblasts cell proliferation cytokine secretion |
| title | Biological Impact of γ-Fe<sub>2</sub>O<sub>3</sub> Magnetic Nanoparticles Obtained by Laser Target Evaporation: Focus on Magnetic Biosensor Applications |
| title_full | Biological Impact of γ-Fe<sub>2</sub>O<sub>3</sub> Magnetic Nanoparticles Obtained by Laser Target Evaporation: Focus on Magnetic Biosensor Applications |
| title_fullStr | Biological Impact of γ-Fe<sub>2</sub>O<sub>3</sub> Magnetic Nanoparticles Obtained by Laser Target Evaporation: Focus on Magnetic Biosensor Applications |
| title_full_unstemmed | Biological Impact of γ-Fe<sub>2</sub>O<sub>3</sub> Magnetic Nanoparticles Obtained by Laser Target Evaporation: Focus on Magnetic Biosensor Applications |
| title_short | Biological Impact of γ-Fe<sub>2</sub>O<sub>3</sub> Magnetic Nanoparticles Obtained by Laser Target Evaporation: Focus on Magnetic Biosensor Applications |
| title_sort | biological impact of γ fe sub 2 sub o sub 3 sub magnetic nanoparticles obtained by laser target evaporation focus on magnetic biosensor applications |
| topic | magnetic nanoparticles constant magnetic field biomedical applications human skin fibroblasts cell proliferation cytokine secretion |
| url | https://www.mdpi.com/2079-6374/12/8/627 |
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