Potential of ESBL-producing Escherichia coli selection in bovine feces after intramammary administration of first generation cephalosporins using in vitro experiments
Abstract Selection and spread of Extended Spectrum Beta-Lactamase (ESBL) -producing Enterobacteriaceae within animal production systems and potential spillover to humans is a major concern. Intramammary treatment of dairy cows with first-generation cephalosporins is a common practice and potentially...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-15558-z |
| _version_ | 1818064641552547840 |
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| author | David C. Speksnijder Nonke E. M. Hopman Nina E. Kusters Arjen Timmerman Jantijn M. Swinkels Pleun A. A. Penterman Volker Krömker Andrew J. Bradley Nadine Botteldoorn Ronette Gehring Aldert L. Zomer |
| author_facet | David C. Speksnijder Nonke E. M. Hopman Nina E. Kusters Arjen Timmerman Jantijn M. Swinkels Pleun A. A. Penterman Volker Krömker Andrew J. Bradley Nadine Botteldoorn Ronette Gehring Aldert L. Zomer |
| author_sort | David C. Speksnijder |
| collection | DOAJ |
| description | Abstract Selection and spread of Extended Spectrum Beta-Lactamase (ESBL) -producing Enterobacteriaceae within animal production systems and potential spillover to humans is a major concern. Intramammary treatment of dairy cows with first-generation cephalosporins is a common practice and potentially selects for ESBL-producing Enterobacteriaceae, although it is unknown whether this really occurs in the bovine fecal environment. We aimed to study the potential effects of intramammary application of cephapirin (CP) and cefalonium (CL) to select for ESBL-producing Escherichia coli in the intestinal content of treated dairy cows and in manure slurry, using in vitro competition experiments with ESBL and non-ESBL E. coli isolates. No selection of ESBL-producing E. coli was observed at or below concentrations of 0.8 µg/ml and 4.0 µg/ml in bovine feces for CP and CL, respectively, and at or below 8.0 µg/ml and 4.0 µg/ml, respectively, in manure slurry. We calculated that the maximum concentration of CP and CL after intramammary treatment with commercial products will not exceed 0.29 µg/ml in feces and 0.03 µg/ml in manure slurry. Therefore, the results of this study did not find evidence supporting the selection of ESBL-producing E. coli in bovine feces or in manure slurry after intramammary use of commercial CP or CL-containing products. |
| first_indexed | 2024-12-10T14:39:14Z |
| format | Article |
| id | doaj.art-793f320434124e37ab890f66c8facc3f |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-10T14:39:14Z |
| publishDate | 2022-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-793f320434124e37ab890f66c8facc3f2022-12-22T01:44:44ZengNature PortfolioScientific Reports2045-23222022-09-0112111010.1038/s41598-022-15558-zPotential of ESBL-producing Escherichia coli selection in bovine feces after intramammary administration of first generation cephalosporins using in vitro experimentsDavid C. Speksnijder0Nonke E. M. Hopman1Nina E. Kusters2Arjen Timmerman3Jantijn M. Swinkels4Pleun A. A. Penterman5Volker Krömker6Andrew J. Bradley7Nadine Botteldoorn8Ronette Gehring9Aldert L. Zomer10Division Infectious Diseases & Immunology, Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht UniversityDivision Infectious Diseases & Immunology, Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht UniversityDivision Infectious Diseases & Immunology, Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht UniversityDivision Infectious Diseases & Immunology, Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht UniversityGlobal Ruminant Business Unit, MSD Animal HealthMSD Animal HealthDepartment of Veterinary and Animal Sciences, University of CopenhagenQuality Milk Management Services LtdAnimal Health Care FlandersDepartment of Population Health Sciences, Faculty of Veterinary Medicine, Institute for Risk Assessment Sciences, Utrecht UniversityDivision Infectious Diseases & Immunology, Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht UniversityAbstract Selection and spread of Extended Spectrum Beta-Lactamase (ESBL) -producing Enterobacteriaceae within animal production systems and potential spillover to humans is a major concern. Intramammary treatment of dairy cows with first-generation cephalosporins is a common practice and potentially selects for ESBL-producing Enterobacteriaceae, although it is unknown whether this really occurs in the bovine fecal environment. We aimed to study the potential effects of intramammary application of cephapirin (CP) and cefalonium (CL) to select for ESBL-producing Escherichia coli in the intestinal content of treated dairy cows and in manure slurry, using in vitro competition experiments with ESBL and non-ESBL E. coli isolates. No selection of ESBL-producing E. coli was observed at or below concentrations of 0.8 µg/ml and 4.0 µg/ml in bovine feces for CP and CL, respectively, and at or below 8.0 µg/ml and 4.0 µg/ml, respectively, in manure slurry. We calculated that the maximum concentration of CP and CL after intramammary treatment with commercial products will not exceed 0.29 µg/ml in feces and 0.03 µg/ml in manure slurry. Therefore, the results of this study did not find evidence supporting the selection of ESBL-producing E. coli in bovine feces or in manure slurry after intramammary use of commercial CP or CL-containing products.https://doi.org/10.1038/s41598-022-15558-z |
| spellingShingle | David C. Speksnijder Nonke E. M. Hopman Nina E. Kusters Arjen Timmerman Jantijn M. Swinkels Pleun A. A. Penterman Volker Krömker Andrew J. Bradley Nadine Botteldoorn Ronette Gehring Aldert L. Zomer Potential of ESBL-producing Escherichia coli selection in bovine feces after intramammary administration of first generation cephalosporins using in vitro experiments Scientific Reports |
| title | Potential of ESBL-producing Escherichia coli selection in bovine feces after intramammary administration of first generation cephalosporins using in vitro experiments |
| title_full | Potential of ESBL-producing Escherichia coli selection in bovine feces after intramammary administration of first generation cephalosporins using in vitro experiments |
| title_fullStr | Potential of ESBL-producing Escherichia coli selection in bovine feces after intramammary administration of first generation cephalosporins using in vitro experiments |
| title_full_unstemmed | Potential of ESBL-producing Escherichia coli selection in bovine feces after intramammary administration of first generation cephalosporins using in vitro experiments |
| title_short | Potential of ESBL-producing Escherichia coli selection in bovine feces after intramammary administration of first generation cephalosporins using in vitro experiments |
| title_sort | potential of esbl producing escherichia coli selection in bovine feces after intramammary administration of first generation cephalosporins using in vitro experiments |
| url | https://doi.org/10.1038/s41598-022-15558-z |
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