Improvement of the Transglycosylation Efficiency of a Lacto-<i>N</i>-Biosidase from <i>Bifidobacterium bifidum</i> by Protein Engineering

Improvement of the Transglycosylation Efficiency of a Lacto-<i>N</i>-Biosidase from <i>Bifidobacterium bifidum</i> by Protein Engineering

The lacto-<i>N</i>-biosidase LnbB from <i>Bifidobacterium bifidum</i> JCM 1254 was engineered to improve its negligible transglycosylation efficiency with the purpose of enzymatically synthesizing lacto-<i>N</i>-tetraose (LNT; Gal-β1,3-GlcNAc-β1,3-Gal-β1,4-Glc) in...

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Bibliographic Details
Main Authors: Marlene Vuillemin, Jesper Holck, Martin Matwiejuk, Eduardo S. Moreno Prieto, Jan Muschiol, Dora Molnar-Gabor, Anne S. Meyer, Birgitte Zeuner
Format: Article
Language:English
Published: MDPI AG 2021-12-01
Series:Applied Sciences
Subjects:
lacto-<i>N</i>-biosidase
GH20
transglycosylation
protein engineering
lacto-<i>N</i>-tetraose
Online Access:https://www.mdpi.com/2076-3417/11/23/11493

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