Reproducibility of Circulating MicroRNAs in Stored Plasma Samples.
Most studies of microRNA (miRNA) and disease have examined tissue-specific expression in limited numbers of samples. The presence of circulating miRNAs in plasma samples provides the opportunity to examine prospective associations between miRNA expression and disease in initially healthy individuals...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4552013?pdf=render |
| _version_ | 1818942453343846400 |
|---|---|
| author | Monica L Bertoia Kimberly A Bertrand Sherilyn J Sawyer Eric B Rimm Kenneth J Mukamal |
| author_facet | Monica L Bertoia Kimberly A Bertrand Sherilyn J Sawyer Eric B Rimm Kenneth J Mukamal |
| author_sort | Monica L Bertoia |
| collection | DOAJ |
| description | Most studies of microRNA (miRNA) and disease have examined tissue-specific expression in limited numbers of samples. The presence of circulating miRNAs in plasma samples provides the opportunity to examine prospective associations between miRNA expression and disease in initially healthy individuals. However, little data exist on the reproducibility of miRNAs in stored plasma.We used Real-Time PCR to measure 61 pre-selected microRNA candidates in stored plasma. Coefficients of variation (CVs) were used to assess inter-assay reliability (n = 15) and within-person stability over one year (n = 80). Intraclass correlation coefficients (ICCs) and polychoric correlation coefficients were used to assess within-person stability and delayed processing reproducibility (whole blood stored at 4°C for 0, 24 and 48 hours; n = 12 samples).Of 61 selected miRNAs, 23 were detected in at least 50% of samples and had average CVs below 20% for inter-assay reproducibility and 31 for delayed processing reproducibility. Ten miRNAs were detected in at least 50% of samples, had average CVs below 20% and had ICCs above 0.4 for within-person stability over 1-2 years, six of which satisfied criteria for both interassay reproducibility and short-term within-person stability (miR-17-5p, -191-5p, -26a-5p, -27b-3p, -320a, and -375) and two all three types of reproducibility (miR-27b-3p and -26a-5p). However, many miRNAs with acceptable average CVs had high maximum CVs, most had low expression levels, and several had low ICCs with delayed processing.About a tenth of miRNAs plausibly related to chronic disease were reliably detected in stored samples of healthy adults. |
| first_indexed | 2024-12-20T07:11:40Z |
| format | Article |
| id | doaj.art-7942fb23ca9646a6bbfead251d3956d8 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T07:11:40Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-7942fb23ca9646a6bbfead251d3956d82022-12-21T19:48:53ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01108e013666510.1371/journal.pone.0136665Reproducibility of Circulating MicroRNAs in Stored Plasma Samples.Monica L BertoiaKimberly A BertrandSherilyn J SawyerEric B RimmKenneth J MukamalMost studies of microRNA (miRNA) and disease have examined tissue-specific expression in limited numbers of samples. The presence of circulating miRNAs in plasma samples provides the opportunity to examine prospective associations between miRNA expression and disease in initially healthy individuals. However, little data exist on the reproducibility of miRNAs in stored plasma.We used Real-Time PCR to measure 61 pre-selected microRNA candidates in stored plasma. Coefficients of variation (CVs) were used to assess inter-assay reliability (n = 15) and within-person stability over one year (n = 80). Intraclass correlation coefficients (ICCs) and polychoric correlation coefficients were used to assess within-person stability and delayed processing reproducibility (whole blood stored at 4°C for 0, 24 and 48 hours; n = 12 samples).Of 61 selected miRNAs, 23 were detected in at least 50% of samples and had average CVs below 20% for inter-assay reproducibility and 31 for delayed processing reproducibility. Ten miRNAs were detected in at least 50% of samples, had average CVs below 20% and had ICCs above 0.4 for within-person stability over 1-2 years, six of which satisfied criteria for both interassay reproducibility and short-term within-person stability (miR-17-5p, -191-5p, -26a-5p, -27b-3p, -320a, and -375) and two all three types of reproducibility (miR-27b-3p and -26a-5p). However, many miRNAs with acceptable average CVs had high maximum CVs, most had low expression levels, and several had low ICCs with delayed processing.About a tenth of miRNAs plausibly related to chronic disease were reliably detected in stored samples of healthy adults.http://europepmc.org/articles/PMC4552013?pdf=render |
| spellingShingle | Monica L Bertoia Kimberly A Bertrand Sherilyn J Sawyer Eric B Rimm Kenneth J Mukamal Reproducibility of Circulating MicroRNAs in Stored Plasma Samples. PLoS ONE |
| title | Reproducibility of Circulating MicroRNAs in Stored Plasma Samples. |
| title_full | Reproducibility of Circulating MicroRNAs in Stored Plasma Samples. |
| title_fullStr | Reproducibility of Circulating MicroRNAs in Stored Plasma Samples. |
| title_full_unstemmed | Reproducibility of Circulating MicroRNAs in Stored Plasma Samples. |
| title_short | Reproducibility of Circulating MicroRNAs in Stored Plasma Samples. |
| title_sort | reproducibility of circulating micrornas in stored plasma samples |
| url | http://europepmc.org/articles/PMC4552013?pdf=render |
| work_keys_str_mv | AT monicalbertoia reproducibilityofcirculatingmicrornasinstoredplasmasamples AT kimberlyabertrand reproducibilityofcirculatingmicrornasinstoredplasmasamples AT sherilynjsawyer reproducibilityofcirculatingmicrornasinstoredplasmasamples AT ericbrimm reproducibilityofcirculatingmicrornasinstoredplasmasamples AT kennethjmukamal reproducibilityofcirculatingmicrornasinstoredplasmasamples |