Sodium tanshinone IIA sulfonate stimulated Cl- secretion in mouse trachea.
Sodium tanshinone IIA sulfonate (STS) is a derivate of tanshinone IIA, a lipophilic compound in Salvia miltiorrhiza. This study aimed to investigate the effect of STS on ion transport in mouse tracheal epithelium and the mechanisms underlying it. Short-circuit current (Isc) was measured to evaluate...
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5440052?pdf=render |
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| author | Peng-Xiao Chen Yi-Lin Zhang Jia-Wen Xu Ming-Hao Yu Jie-Hong Huang Lei Zhao Wen-Liang Zhou |
| author_facet | Peng-Xiao Chen Yi-Lin Zhang Jia-Wen Xu Ming-Hao Yu Jie-Hong Huang Lei Zhao Wen-Liang Zhou |
| author_sort | Peng-Xiao Chen |
| collection | DOAJ |
| description | Sodium tanshinone IIA sulfonate (STS) is a derivate of tanshinone IIA, a lipophilic compound in Salvia miltiorrhiza. This study aimed to investigate the effect of STS on ion transport in mouse tracheal epithelium and the mechanisms underlying it. Short-circuit current (Isc) was measured to evaluate the effect of STS on transepithelial ion transport. Intracellular Ca2+ imaging was performed to observe intracellular Ca2+ concentration ([Ca2+]i) changes induced by STS in primary cultured mouse tracheal epithelial cells. Results showed that the apical application of STS at mouse trachea elicited an increase of Isc, which was abrogated by atropine, an antagonist of muscarinic acetylcholine receptor (mAChR). By removing ambient Cl- or applying blockers of Ca2+-activated Cl- channel (CaCC), the response of STS-induced Isc was suppressed. Moreover, STS elevated the [Ca2+]i in mouse tracheal epithelial cells. As a result, STS stimulated Cl- secretion in mouse tracheal epithelium via CaCC in an mAChR-dependent way. Due to the critical role of Cl- secretion in airway hydration, our findings suggested that STS may be used to ameliorate the airway dehydration symptom in cystic fibrosis (CF) and chronic obstructive pulmonary disease (COPD). |
| first_indexed | 2024-04-12T01:13:31Z |
| format | Article |
| id | doaj.art-7943c4d171f94d0f962268fe95746462 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T01:13:31Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj.art-7943c4d171f94d0f962268fe957464622022-12-22T03:54:03ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01125e017822610.1371/journal.pone.0178226Sodium tanshinone IIA sulfonate stimulated Cl- secretion in mouse trachea.Peng-Xiao ChenYi-Lin ZhangJia-Wen XuMing-Hao YuJie-Hong HuangLei ZhaoWen-Liang ZhouSodium tanshinone IIA sulfonate (STS) is a derivate of tanshinone IIA, a lipophilic compound in Salvia miltiorrhiza. This study aimed to investigate the effect of STS on ion transport in mouse tracheal epithelium and the mechanisms underlying it. Short-circuit current (Isc) was measured to evaluate the effect of STS on transepithelial ion transport. Intracellular Ca2+ imaging was performed to observe intracellular Ca2+ concentration ([Ca2+]i) changes induced by STS in primary cultured mouse tracheal epithelial cells. Results showed that the apical application of STS at mouse trachea elicited an increase of Isc, which was abrogated by atropine, an antagonist of muscarinic acetylcholine receptor (mAChR). By removing ambient Cl- or applying blockers of Ca2+-activated Cl- channel (CaCC), the response of STS-induced Isc was suppressed. Moreover, STS elevated the [Ca2+]i in mouse tracheal epithelial cells. As a result, STS stimulated Cl- secretion in mouse tracheal epithelium via CaCC in an mAChR-dependent way. Due to the critical role of Cl- secretion in airway hydration, our findings suggested that STS may be used to ameliorate the airway dehydration symptom in cystic fibrosis (CF) and chronic obstructive pulmonary disease (COPD).http://europepmc.org/articles/PMC5440052?pdf=render |
| spellingShingle | Peng-Xiao Chen Yi-Lin Zhang Jia-Wen Xu Ming-Hao Yu Jie-Hong Huang Lei Zhao Wen-Liang Zhou Sodium tanshinone IIA sulfonate stimulated Cl- secretion in mouse trachea. PLoS ONE |
| title | Sodium tanshinone IIA sulfonate stimulated Cl- secretion in mouse trachea. |
| title_full | Sodium tanshinone IIA sulfonate stimulated Cl- secretion in mouse trachea. |
| title_fullStr | Sodium tanshinone IIA sulfonate stimulated Cl- secretion in mouse trachea. |
| title_full_unstemmed | Sodium tanshinone IIA sulfonate stimulated Cl- secretion in mouse trachea. |
| title_short | Sodium tanshinone IIA sulfonate stimulated Cl- secretion in mouse trachea. |
| title_sort | sodium tanshinone iia sulfonate stimulated cl secretion in mouse trachea |
| url | http://europepmc.org/articles/PMC5440052?pdf=render |
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