Oral Microbiome Metabarcoding in Two Invasive Small Mammals from New Zealand
All multicellular organisms host a wide diversity of microorganisms in and on their bodies, which are collectively known as their microbiome. Characterising microbial communities that inhabit different body niches in wild animals is critical to better understand the dynamics of microbiome diversitya...
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MDPI AG
2020-07-01
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| Online Access: | https://www.mdpi.com/1424-2818/12/7/278 |
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| author | Arsalan Emami-Khoyi Isma Benmazouz Adrian M. Paterson James G. Ross Elaine C. Murphy Jennifer Bothwell Hossein Alizadeh Bettine Jansen van Vuuren Peter R. Teske |
| author_facet | Arsalan Emami-Khoyi Isma Benmazouz Adrian M. Paterson James G. Ross Elaine C. Murphy Jennifer Bothwell Hossein Alizadeh Bettine Jansen van Vuuren Peter R. Teske |
| author_sort | Arsalan Emami-Khoyi |
| collection | DOAJ |
| description | All multicellular organisms host a wide diversity of microorganisms in and on their bodies, which are collectively known as their microbiome. Characterising microbial communities that inhabit different body niches in wild animals is critical to better understand the dynamics of microbiome diversityand its functional significance. The current study is the first to apply massively parallel sequencing of 16S rRNA to characterise the microbial diversity and functional content of oral microbiota in two of New Zealand’s most important invasive mammals, the omnivorous common brushtail possum (<i>Trichosurus vulpecula</i>) and the carnivorous stoat (<i>Mustela erminea</i>). In total, strains of bacteria belonging to 19 different phyla, 27 classes, 52 orders, 103 families, 163 genera and 51 known species were identified from the oral cavities of the study species. Strains of the phyla Proteobacteria, Firmicutes, Bacteroidetes, Fusobacteria, and Actinobacteria dominated the core oral microbial diversity in both species, while other taxa were comparatively less abundant. Despite invasive populations typically demonstrating limited genetic variation, intraspecific variation of the core bacterial taxa in the oral microbiota was considerable. This suggests that a complex interaction between genetic, physiological, and environmental factors determines the diversity of the study species’oral microbiome. |
| first_indexed | 2024-03-10T18:33:32Z |
| format | Article |
| id | doaj.art-7945afe3e18d40ceb15daff2ccc0718a |
| institution | Directory Open Access Journal |
| issn | 1424-2818 |
| language | English |
| last_indexed | 2024-03-10T18:33:32Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diversity |
| spelling | doaj.art-7945afe3e18d40ceb15daff2ccc0718a2023-11-20T06:25:05ZengMDPI AGDiversity1424-28182020-07-0112727810.3390/d12070278Oral Microbiome Metabarcoding in Two Invasive Small Mammals from New ZealandArsalan Emami-Khoyi0Isma Benmazouz1Adrian M. Paterson2James G. Ross3Elaine C. Murphy4Jennifer Bothwell5Hossein Alizadeh6Bettine Jansen van Vuuren7Peter R. Teske8Center for Ecological Genomics and Wildlife Conservation, Department of Zoology, University of Johannesburg, Auckland Park 2006, South AfricaInstitute of Wildlife Conservation, SzentIstván University, 2100 Gödöllő, HungaryDepartment of Pest-Management and Conservation, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New ZealandDepartment of Pest-Management and Conservation, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New ZealandDepartment of Pest-Management and Conservation, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New ZealandDepartment of Pest-Management and Conservation, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New ZealandBio-Protection Research Centre, Lincoln University, Lincoln 7647, New ZealandCenter for Ecological Genomics and Wildlife Conservation, Department of Zoology, University of Johannesburg, Auckland Park 2006, South AfricaCenter for Ecological Genomics and Wildlife Conservation, Department of Zoology, University of Johannesburg, Auckland Park 2006, South AfricaAll multicellular organisms host a wide diversity of microorganisms in and on their bodies, which are collectively known as their microbiome. Characterising microbial communities that inhabit different body niches in wild animals is critical to better understand the dynamics of microbiome diversityand its functional significance. The current study is the first to apply massively parallel sequencing of 16S rRNA to characterise the microbial diversity and functional content of oral microbiota in two of New Zealand’s most important invasive mammals, the omnivorous common brushtail possum (<i>Trichosurus vulpecula</i>) and the carnivorous stoat (<i>Mustela erminea</i>). In total, strains of bacteria belonging to 19 different phyla, 27 classes, 52 orders, 103 families, 163 genera and 51 known species were identified from the oral cavities of the study species. Strains of the phyla Proteobacteria, Firmicutes, Bacteroidetes, Fusobacteria, and Actinobacteria dominated the core oral microbial diversity in both species, while other taxa were comparatively less abundant. Despite invasive populations typically demonstrating limited genetic variation, intraspecific variation of the core bacterial taxa in the oral microbiota was considerable. This suggests that a complex interaction between genetic, physiological, and environmental factors determines the diversity of the study species’oral microbiome.https://www.mdpi.com/1424-2818/12/7/278microbiomeoral cavitymicrobiotacommon brushtail possumstoatinvasive species |
| spellingShingle | Arsalan Emami-Khoyi Isma Benmazouz Adrian M. Paterson James G. Ross Elaine C. Murphy Jennifer Bothwell Hossein Alizadeh Bettine Jansen van Vuuren Peter R. Teske Oral Microbiome Metabarcoding in Two Invasive Small Mammals from New Zealand Diversity microbiome oral cavity microbiota common brushtail possum stoat invasive species |
| title | Oral Microbiome Metabarcoding in Two Invasive Small Mammals from New Zealand |
| title_full | Oral Microbiome Metabarcoding in Two Invasive Small Mammals from New Zealand |
| title_fullStr | Oral Microbiome Metabarcoding in Two Invasive Small Mammals from New Zealand |
| title_full_unstemmed | Oral Microbiome Metabarcoding in Two Invasive Small Mammals from New Zealand |
| title_short | Oral Microbiome Metabarcoding in Two Invasive Small Mammals from New Zealand |
| title_sort | oral microbiome metabarcoding in two invasive small mammals from new zealand |
| topic | microbiome oral cavity microbiota common brushtail possum stoat invasive species |
| url | https://www.mdpi.com/1424-2818/12/7/278 |
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