Genotype classification of Moraxella bovis using MALDI-TOF MS profiles
Moraxella bovis (M. bovis) is regarded as a causative agent of infectious bovine keratoconjunctivitis (IBK), the most common ocular disease of cattle. Recently, whole genome sequencing identified the presence of two distinct genotypes within M. bovis that differ in chromosome content, potential viru...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-12-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1057621/full |
| _version_ | 1811205291131273216 |
|---|---|
| author | Hannah G. Olson John Dustin Loy Michael L. Clawson Emily L. Wynn Matthew M. Hille |
| author_facet | Hannah G. Olson John Dustin Loy Michael L. Clawson Emily L. Wynn Matthew M. Hille |
| author_sort | Hannah G. Olson |
| collection | DOAJ |
| description | Moraxella bovis (M. bovis) is regarded as a causative agent of infectious bovine keratoconjunctivitis (IBK), the most common ocular disease of cattle. Recently, whole genome sequencing identified the presence of two distinct genotypes within M. bovis that differ in chromosome content, potential virulence factors, as well as prophage and plasmid profiles. It is unclear if the genotypes equally associate with IBK or if one is more likely to be isolated from IBK lesions. We utilized 39 strains of M. bovis that had previously undergone whole genome sequencing and genotype classification to determine the utility of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) to accurately genotype M. bovis strains. We successfully developed two biomarker models that accurately classified strains according to genotype with an overall accuracy of 85.8–100% depending upon the model and sample preparation method used. These models provide a practical tool to enable studies of genotype associations with disease, allow for epidemiological studies at the sub-species level, and can be used to enhance disease prevention strategies. |
| first_indexed | 2024-04-12T03:28:30Z |
| format | Article |
| id | doaj.art-79466915eea8455abef1e8364d08790d |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T03:28:30Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-79466915eea8455abef1e8364d08790d2022-12-22T03:49:36ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-12-011310.3389/fmicb.2022.10576211057621Genotype classification of Moraxella bovis using MALDI-TOF MS profilesHannah G. Olson0John Dustin Loy1Michael L. Clawson2Emily L. Wynn3Matthew M. Hille4School of Veterinary Medicine and Biomedical Sciences, Institute for Agriculture and Natural Resources, University of Nebraska-Lincoln, Lincoln, NE, United StatesSchool of Veterinary Medicine and Biomedical Sciences, Institute for Agriculture and Natural Resources, University of Nebraska-Lincoln, Lincoln, NE, United StatesUnited States Department of Agricultural, Agricultural Research Service, United States Meat Animal Research Center, Clay Center, NE, United StatesUnited States Department of Agricultural, Agricultural Research Service, United States Meat Animal Research Center, Clay Center, NE, United StatesSchool of Veterinary Medicine and Biomedical Sciences, Institute for Agriculture and Natural Resources, University of Nebraska-Lincoln, Lincoln, NE, United StatesMoraxella bovis (M. bovis) is regarded as a causative agent of infectious bovine keratoconjunctivitis (IBK), the most common ocular disease of cattle. Recently, whole genome sequencing identified the presence of two distinct genotypes within M. bovis that differ in chromosome content, potential virulence factors, as well as prophage and plasmid profiles. It is unclear if the genotypes equally associate with IBK or if one is more likely to be isolated from IBK lesions. We utilized 39 strains of M. bovis that had previously undergone whole genome sequencing and genotype classification to determine the utility of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) to accurately genotype M. bovis strains. We successfully developed two biomarker models that accurately classified strains according to genotype with an overall accuracy of 85.8–100% depending upon the model and sample preparation method used. These models provide a practical tool to enable studies of genotype associations with disease, allow for epidemiological studies at the sub-species level, and can be used to enhance disease prevention strategies.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1057621/fullMoraxella bovisMALDI-TOF MSinfectious bovine keratoconjunctivitisbovine pinkeyegenotype |
| spellingShingle | Hannah G. Olson John Dustin Loy Michael L. Clawson Emily L. Wynn Matthew M. Hille Genotype classification of Moraxella bovis using MALDI-TOF MS profiles Frontiers in Microbiology Moraxella bovis MALDI-TOF MS infectious bovine keratoconjunctivitis bovine pinkeye genotype |
| title | Genotype classification of Moraxella bovis using MALDI-TOF MS profiles |
| title_full | Genotype classification of Moraxella bovis using MALDI-TOF MS profiles |
| title_fullStr | Genotype classification of Moraxella bovis using MALDI-TOF MS profiles |
| title_full_unstemmed | Genotype classification of Moraxella bovis using MALDI-TOF MS profiles |
| title_short | Genotype classification of Moraxella bovis using MALDI-TOF MS profiles |
| title_sort | genotype classification of moraxella bovis using maldi tof ms profiles |
| topic | Moraxella bovis MALDI-TOF MS infectious bovine keratoconjunctivitis bovine pinkeye genotype |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1057621/full |
| work_keys_str_mv | AT hannahgolson genotypeclassificationofmoraxellabovisusingmalditofmsprofiles AT johndustinloy genotypeclassificationofmoraxellabovisusingmalditofmsprofiles AT michaellclawson genotypeclassificationofmoraxellabovisusingmalditofmsprofiles AT emilylwynn genotypeclassificationofmoraxellabovisusingmalditofmsprofiles AT matthewmhille genotypeclassificationofmoraxellabovisusingmalditofmsprofiles |