Application of a Fluorescent Biosensor in Determining the Binding of 5-HT to Calmodulin

Application of a Fluorescent Biosensor in Determining the Binding of 5-HT to Calmodulin

Here, we show the utility of the fluorescent biosensor <i>h</i>CaM-M124C-<i>mBBr</i> in detecting and determining the affinity of serotonin (5-HT). We obtained a <i>K</i><sub>d</sub> of 5-HT (0.71 μm) for the first time, the same order of magnitude as...

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Bibliographic Details
Main Authors: L. X. Vásquez-Bochm, Isabel Velázquez-López, Rachel Mata, Alejandro Sosa-Peinado, Patricia Cano-Sánchez, Martin González-Andrade
Format: Article
Language:English
Published: MDPI AG 2021-09-01
Series:Chemosensors
Subjects:
biosensor
calmodulin
5-HT
docking
molecular dynamic simulation
Online Access:https://www.mdpi.com/2227-9040/9/9/250

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