Generation of the iPSC line IISHDOi007-A from peripheral blood mononuclear cells from a patient with McArdle disease harbouring the mutation c.2392 T > C; p.Trp798Arg
Peripheral blood mononuclear cells (PBMCs) from a McArdle patient carrying a homozygous mutation in the PYGM gene: c.2392 T > C; p.Trp798Arg were used for the generation of the human iPSC line, IISHDOi007-A. For the delivery of the reprogramming factors Oct3/4, Sox2, Klf4, and c-Myc, a non-integr...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-12-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506120304098 |
| _version_ | 1818393214799839232 |
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| author | Victoria Cerrada Marta García-López Sofía Alvarez-Galeano Ana Moreno-Izquierdo Alejandro Lucia María Rabasa Pérez Joaquín Arenas M. Esther Gallardo |
| author_facet | Victoria Cerrada Marta García-López Sofía Alvarez-Galeano Ana Moreno-Izquierdo Alejandro Lucia María Rabasa Pérez Joaquín Arenas M. Esther Gallardo |
| author_sort | Victoria Cerrada |
| collection | DOAJ |
| description | Peripheral blood mononuclear cells (PBMCs) from a McArdle patient carrying a homozygous mutation in the PYGM gene: c.2392 T > C; p.Trp798Arg were used for the generation of the human iPSC line, IISHDOi007-A. For the delivery of the reprogramming factors Oct3/4, Sox2, Klf4, and c-Myc, a non-integrative methodology that implies the use of Sendai virus has been applied. |
| first_indexed | 2024-12-14T05:41:46Z |
| format | Article |
| id | doaj.art-794ddd10f01b43a8bbd4a4ec8227656d |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-12-14T05:41:46Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-794ddd10f01b43a8bbd4a4ec8227656d2022-12-21T23:15:00ZengElsevierStem Cell Research1873-50612020-12-0149102108Generation of the iPSC line IISHDOi007-A from peripheral blood mononuclear cells from a patient with McArdle disease harbouring the mutation c.2392 T > C; p.Trp798ArgVictoria Cerrada0Marta García-López1Sofía Alvarez-Galeano2Ana Moreno-Izquierdo3Alejandro Lucia4María Rabasa Pérez5Joaquín Arenas6M. Esther Gallardo7Grupo de Investigación Traslacional con Células iPS, Instituto de Investigación Sanitaria Hospital 12 de Octubre (i+12), Madrid, SpainGrupo de Investigación Traslacional con Células iPS, Instituto de Investigación Sanitaria Hospital 12 de Octubre (i+12), Madrid, SpainGrupo de Investigación Traslacional con Células iPS, Instituto de Investigación Sanitaria Hospital 12 de Octubre (i+12), Madrid, SpainServicio de Genética. Instituto de Investigación Sanitaria Hospital 12 de Octubre (i+12), Madrid, SpainFaculty of Sports Sciences, Universidad Europea de Madrid, Madrid, SpainNeurología, Hospital Universitario de Fuenlabrada, Fuenlabrada, Madrid, SpainGrupo de Enfermedades Raras, Mitocondriales y neuromusculares. Instituto de Investigación Sanitaria Hospital 12 de Octubre (i+12), Madrid, Spain; Centro de Investigación Biomédica en Red (CIBERER), Madrid, SpainGrupo de Investigación Traslacional con Células iPS, Instituto de Investigación Sanitaria Hospital 12 de Octubre (i+12), Madrid, Spain; Centro de Investigación Biomédica en Red (CIBERER), Madrid, Spain; Corresponding author at: Grupo de Investigación Traslacional con células iPS, Instituto de Investigación Sanitaria Hospital 12 de Octubre (i+12), Madrid, Spain.Peripheral blood mononuclear cells (PBMCs) from a McArdle patient carrying a homozygous mutation in the PYGM gene: c.2392 T > C; p.Trp798Arg were used for the generation of the human iPSC line, IISHDOi007-A. For the delivery of the reprogramming factors Oct3/4, Sox2, Klf4, and c-Myc, a non-integrative methodology that implies the use of Sendai virus has been applied.http://www.sciencedirect.com/science/article/pii/S1873506120304098 |
| spellingShingle | Victoria Cerrada Marta García-López Sofía Alvarez-Galeano Ana Moreno-Izquierdo Alejandro Lucia María Rabasa Pérez Joaquín Arenas M. Esther Gallardo Generation of the iPSC line IISHDOi007-A from peripheral blood mononuclear cells from a patient with McArdle disease harbouring the mutation c.2392 T > C; p.Trp798Arg Stem Cell Research |
| title | Generation of the iPSC line IISHDOi007-A from peripheral blood mononuclear cells from a patient with McArdle disease harbouring the mutation c.2392 T > C; p.Trp798Arg |
| title_full | Generation of the iPSC line IISHDOi007-A from peripheral blood mononuclear cells from a patient with McArdle disease harbouring the mutation c.2392 T > C; p.Trp798Arg |
| title_fullStr | Generation of the iPSC line IISHDOi007-A from peripheral blood mononuclear cells from a patient with McArdle disease harbouring the mutation c.2392 T > C; p.Trp798Arg |
| title_full_unstemmed | Generation of the iPSC line IISHDOi007-A from peripheral blood mononuclear cells from a patient with McArdle disease harbouring the mutation c.2392 T > C; p.Trp798Arg |
| title_short | Generation of the iPSC line IISHDOi007-A from peripheral blood mononuclear cells from a patient with McArdle disease harbouring the mutation c.2392 T > C; p.Trp798Arg |
| title_sort | generation of the ipsc line iishdoi007 a from peripheral blood mononuclear cells from a patient with mcardle disease harbouring the mutation c 2392 t c p trp798arg |
| url | http://www.sciencedirect.com/science/article/pii/S1873506120304098 |
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